Automatic treatment device for pre-treatment of nucleic acid detection

An automatic device and nucleic acid technology, which is applied in biochemical cleaning devices, biomass post-processing, and biological material pretreatment, etc., can solve the problems of sample influence and inability to realize unattended operation, so as to avoid tediousness, eliminate sample confusion, and simplify The effect of the test procedure

Pending Publication Date: 2017-05-31
HANGZHOU GENE META MEDICAL DEVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the liquid pipetting workstation is an open platform, during the whole multi-step test operation, the operator needs to seal and open the 96-well plate film, or open and close a large number of tube caps from time to time, although part of the work is done by the workstation. Completed, but the overall still requires a lot of manual intervention, and it is impossible to realize the unattended operation of the whole process, and multiple steps need manual marking, sorting, and setting
At the same time, once the test starts, it is impossible to temporarily increase or change the test process. Once there are samples that need to be processed temporarily, or the test process is changed, it will have an impact on the samples participating in the test as a whole.

Method used

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  • Automatic treatment device for pre-treatment of nucleic acid detection
  • Automatic treatment device for pre-treatment of nucleic acid detection
  • Automatic treatment device for pre-treatment of nucleic acid detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1The nucleic acid detection pretreatment automation device shown is composed of a rotating device 1 , a base 2 , a movable magnet 3 , a pipette pump 4 and a pipette tip 5 . The pipette tip 5 is arranged on the pipette pump 4 . The rotating device 1 is arranged on the base 2, and the base 2 is provided with a temperature control module corresponding to the position of the reagent tray. When the rotating device 1 rotates, it drives the temperature control module to rotate together. The base 2 is attached to the reagent tray, and the temperature control module provides temperature control. The reagent disc can be rotated and moved by the rotating device 1 on the base 2 .

[0040] Such as Figure 2-3 The reagent tray shown includes a cover plate 6 and a reagent tray 7. The reagent tray 7 is provided with a number of reagent holes 8, the reagent holes 8 are filled with pretreatment reagents for nucleic acid sequencing, and an aluminum foil sealing film 9 is...

Embodiment 2

[0043] Plasma free DNA is small fragments, fragmented DNA, the fragment length is between 100-300bp, and the peak value is around 160bp. In 1997, Professor Lu Yuming discovered that the peripheral plasma of pregnant women contained fetal cell-free DNA, and thus pioneered the use of cell-free DNA as the source of detection to analyze whether the fetus of a pregnant woman has chromosomal abnormalities. Circulating tumor DNA (ctDNA) derived from tumor cells also exists in plasma. ctDNA is widely used for tumor liquid biopsy, which can be used for non-invasive early diagnosis, companion diagnosis, and prognosis follow-up.

[0044] The conventional detection method is to first extract DNA from clinical samples, including plasma, to perform nucleic acid detection, processing, and detection in three steps. It takes 6 to 13 hours to complete the entire sample test. Among them, the extraction of sample nucleic acid and the pre-treatment process of detection are cumbersome.

[0045] A...

Embodiment 3

[0062] The research object of transcriptome sequencing is the sum of all RNAs that can be transcribed by a specific cell in a certain functional state, mainly including mRNA and non-coding RNA. Transcriptome research is the basis and starting point for the study of gene function and structure. Through next-generation high-throughput sequencing, it is possible to comprehensively and quickly obtain almost all transcript sequence information of a specific tissue or organ in a certain state, which has been widely used In basic research, clinical diagnosis and drug development and other fields.

[0063] The conventional detection method is to first extract RNA from clinical samples including whole blood and plasma, and then perform nucleic acid detection processing and detection in three steps. It takes 6 to 30 hours to complete the entire sample test. Among them, the extraction of sample nucleic acid and the pre-treatment process of detection are cumbersome.

[0064] Adopting th...

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Abstract

The invention discloses an automatic treatment device for pre-treatment of nucleic acid detection. The device is characterized by comprising a reagent disc for placing a sample and a pre-treatment reagent, a base for placing the reagent disc, a magnetic sorting module for separating, cleaning and eluting magnetic particles, a pipetting device for transferring the reagent in the reagent disc, and a host machine for controlling the temperature control module, the rotary module, the magnetic sorting module and the pipetting module to operate, wherein a temperature control module and a rotary module are arranged on the base; and the pipetting device is provided with a pipetting module and a pipetting gun head. The automatic treatment device for pre-treatment of nucleic acid detection disclosed by the invention can realize full automation of pre-treatment of nucleic acid detection. The pre-treatment comprises extraction, library construction and product purification. The final product can be directly used for high-throughput sequencing, and the device is of an unattended operation in the all course.

Description

technical field [0001] The invention belongs to the field of nucleic acid molecular detection, and in particular relates to an automatic processing device for nucleic acid detection pretreatment. The present invention realizes fully automatic nucleic acid detection pre-processing, that is, automatically realizes pre-processing such as nucleic acid extraction, amplification, and purification, and the processed samples can be detected by an external matching sequencer. The present invention simplifies the test process, reduces the chance of error, and improves The stability of the overall detection is improved. Background technique [0002] High-throughput sequencing technology is a new molecular detection technology developed in the last decade. The mainstream high-throughput sequencers on the market are mainly provided by Illumina, ThermoFisher, etc. Any high-throughput sequencing platform will first require DNA or RNA to be extracted from biological samples before testing,...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/00
CPCC12Q1/6806C12Q1/6869C12Q2523/308C12Q2531/113C12Q2535/122
Inventor 叶宝春黄飞祝云英
Owner HANGZHOU GENE META MEDICAL DEVICE CO LTD
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