Bio-organic fertilizer, as well as preparation method and application thereof
A bio-organic fertilizer and organic matter technology, which is applied in the field of bio-organic fertilizers, can solve the problems of increasing the yield or quality of bio-organic fertilizers, and achieve the effects of increasing crop yield, increasing soil organic matter content, and reasonable matching
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Embodiment 1
[0022] Preparation of Preliminary Example 1 Lactobacillus acidophilus bacterial agent
[0023] (1) Inoculate Lactobacillus acidophilus (strain number CGMCC1.1854) in beef juice peptone slant medium for culture (3 grams of beef extract, 5-10 grams of peptone, 5 grams of NaCl, distilled water Dissolve the above ingredients to 1000 ml, adjust the pH value to 7), add mineral oil to the slant seed strain of beef juice peptone medium, store in the refrigerator at 4°C, take it out and activate it twice before use.
[0024] (2) Preparation of solid strains
[0025] 1 part of peptone, 0.5 parts of yeast powder, 0.2 parts of ammonium citrate, 2 parts of glucose, 0.054 parts of magnesium sulfate, 1 part of beef extract, 0.2 parts of dipotassium hydrogen phosphate, 0.5 parts of sodium acetate, 0.025 parts of manganese sulfate, 1.5 parts of agar, 93.021 parts of water, natural pH value, divided into test tubes, sterilized at 15 pounds for 20 minutes. Streak inoculation of Lactobacillus p...
Embodiment 3
[0035] Preparation of Preliminary Example 3 jelly-like bacillus bacterial agent
[0036]According to the medium formula and culture temperature provided by the supplier, according to the conventional fermentation method, the jelly-like Bacillus species were cultured on a slant for 18 hours, shaker culture for 14 hours, and fermenter culture for 26 hours under sterile conditions. Obtain jelly-like Bacillus fermented liquid, spray-dry to obtain bacterial powder (powder bacterial agent), the number of effective viable bacteria of the bacterial agent is more than or equal to 1 billion cfu / gram.
[0037] The preparation of embodiment 1 biological organic fertilizer
[0038] Each component (kg) was weighed according to the following weights: wheat straw (dry) 30, Lactobacillus acidophilus inoculum 1, Streptomyces lavender griseus inoculum 1, Bacillus jellylike inoculum 1.
[0039] The dried wheat stalks are pulverized and uniformly mixed with the above three bacterial agents, dried...
Embodiment 2
[0040] The preparation of embodiment 2 biological organic fertilizer
[0041] Weigh each component (kg) according to the following weights: 80 cottonseed meal, 15 Lactobacillus acidophilus inoculum, 10 Streptomyces lavender griseus inoculum, and 10 Bacillus jellylike inoculum.
[0042] The cottonseed meal is pulverized, mixed evenly with the above three bacterial agents, dried and packaged to obtain the finished product.
[0043] The preparation of embodiment 3 biological organic fertilizer
[0044] Each component (kg) was weighed according to the following weights: 70 corn stalks (dry), 6 inoculum agents of Lactobacillus acidophilus, 3 inoculum agents of Streptomyces lavender, and 2 inoculants of Bacillus jellylike.
[0045] The dried corn stalks are pulverized and uniformly mixed with the above three bacterial agents, dried and packaged to obtain the finished product.
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