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Supercritical extraction method for extracting effective components from pine pollen

An active ingredient, supercritical technology, applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, preparations for skin care, etc., can solve the problems of few active ingredients involved, difficult to achieve separation and purification, and use of active ingredients of pine pollen, etc. , to achieve the effect of improving biological activity, promoting utilization, and regulating the physiological function of the skin

Active Publication Date: 2017-05-31
烟台新时代健康产业日化有限公司
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AI Technical Summary

Problems solved by technology

[0003] At present, most of the extraction and separation processes of pine pollen are concentrated in the research of water extraction and alcohol extraction process, and it is difficult to achieve the purpose of separation and purification of the ester components and alkaloid components in pine pollen; The ester components such as sterols, glyceric acid, etc., but the active ingredients involved in the traditional extraction process are rarely involved, so it is difficult to maximize the use of active ingredients in pine pollen

Method used

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Embodiment 1

[0018] A supercritical extraction method for extracting active ingredients from pine pollen, comprising the following steps:

[0019] (1) Extract the total esters: put the pine pollen into the extractor, at a temperature of 40-50°C, a pressure of 25MPa, CO 2 Continuous extraction for 2 hours at a flow rate of 30L / h; with CO extracted from fatty compounds 2 The fluid enters the separation tank I for primary separation at a temperature of 50°C and a pressure of 8 MPa; then enters the separation tank II for secondary separation at a temperature of 30°C and a pressure of 6 MPa;

[0020] (2) extracting the total flavonoids: the drug residue obtained in step (1) is 20-40MPa under pressure, the temperature is 70 ℃, CO 2 Extract for 1.5 hours at a flow rate of 30L / h, and add 200-400ml of ethanol with a concentration of 80-90% as an entrainer during the extraction process;

[0021] (3) Extract the total alkaloid components: mix the drug residue obtained in step (2) with 100 mL of amm...

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Abstract

The invention relates to a supercritical extraction method for extracting effective components from pine pollen. According to the supercritical extraction method, an ester component can play a lipid barrier protection role in skin care products and is closely relevant with efficacies of preserving moisture, resisting inflammation and the like; a flavonoid component has skin care activities of resisting ageing and inflammation and the like; and an alkaloid component has a functional structure group similar to ceramide and is capable of regulating physiological functions of the skin, preserving the moisture and delaying the skin ageing. The functional activities of the three components supplement each other so as to achieve a synergistic interaction effect, so that the utilization rate of the effective components of the pine pollen in terminal products is increased. The invention relates to three-level extraction, except the extraction of common ester components, two substances, namely flavonoid and alkaloid, which are difficultly extracted are extracted from residues by virtue of a supercritical extraction technique, so that the extraction rates of the components, such as flavonoids, are increased, the utilization efficiency of the pine pollen is improved, the waste of resources is avoided, meanwhile, the effective substances in the pine pollen are furthest maintained, and the biological activity is enhanced.

Description

technical field [0001] The invention belongs to the technical field of pine pollen extraction, in particular to a supercritical extraction method for extracting active ingredients from pine pollen. Background technique [0002] Pine pollen is a variety of pollen that can be used both for medicine and food. As a traditional Chinese medicinal material, its history of both medicine and food has exceeded a thousand years, and it has various health effects such as prolonging life. Pollen flavonoids have a variety of beauty and skin care effects, and studies have shown that they have good performance in anti-radiation, anti-aging and anti-oxidation. [0003] At present, most of the extraction and separation processes of pine pollen are concentrated in the research of water extraction and alcohol extraction process, and it is difficult to achieve the purpose of separation and purification of the ester components and alkaloid components in pine pollen; The ester components such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9767A61Q19/00A61Q19/08
CPCA61K8/97A61K2800/805A61Q19/00A61Q19/08
Inventor 陶宇于建伟隋海松卢伟超唐文金潘松浩邹鹏飞
Owner 烟台新时代健康产业日化有限公司
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