Supercritical extraction method for extracting effective components from pine pollen
An active ingredient, supercritical technology, applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, preparations for skin care, etc., can solve the problems of few active ingredients involved, difficult to achieve separation and purification, and use of active ingredients of pine pollen, etc. , to achieve the effect of improving biological activity, promoting utilization, and regulating the physiological function of the skin
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[0018] A supercritical extraction method for extracting active ingredients from pine pollen, comprising the following steps:
[0019] (1) Extract the total esters: put the pine pollen into the extractor, at a temperature of 40-50°C, a pressure of 25MPa, CO 2 Continuous extraction for 2 hours at a flow rate of 30L / h; with CO extracted from fatty compounds 2 The fluid enters the separation tank I for primary separation at a temperature of 50°C and a pressure of 8 MPa; then enters the separation tank II for secondary separation at a temperature of 30°C and a pressure of 6 MPa;
[0020] (2) extracting the total flavonoids: the drug residue obtained in step (1) is 20-40MPa under pressure, the temperature is 70 ℃, CO 2 Extract for 1.5 hours at a flow rate of 30L / h, and add 200-400ml of ethanol with a concentration of 80-90% as an entrainer during the extraction process;
[0021] (3) Extract the total alkaloid components: mix the drug residue obtained in step (2) with 100 mL of amm...
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