Method for extracting yak skin collagen by adopting enzyme process

A skin collagen and enzymatic extraction technology, which is applied in the field of enzymatic extraction of yak skin collagen, can solve the problems of low yak skin utilization value and product added value, so as to improve product value and economic benefits, expand yak utilization range, The effect of high purity

Inactive Publication Date: 2017-05-24
QINGHAI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yak hide is a rich natural resource in the Qinghai-Tibet Plateau, and it is mainly used for leather making, which makes the use value and added value of yak hide generally low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for enzymatically extracting yak skin collagen: 1) Spread a piece of yak skin on the ground with the skin facing down and the quilt up, and smear 1kg of depilatory cream on the quilt (composed of 100 parts of water, 10 parts of vulcanized Sodium and 35 parts of slaked lime), piled up for 4 hours, pushed the hair, washed to get the bare hide;

[0021] 2) Crushing the obtained bare hide into leather particles with a size of 2-5mm;

[0022] 3) Add 100 parts of skin granules into the three-necked bottle, and at the same time add 200% water, 2% soda ash and 1.5% sodium dodecylbenzenesulfonate by weight of the skin granules, and stir at 38°C at a speed of 100r / min Reaction 2.5h;

[0023] 4) After rinsing the skin particles obtained in 3), add 400% water of the weight of the skin particles, 4% baking soda and 0.5% alkaline protease, stir at 38°C and react at a speed of 120r / min for 8 hours to obtain collagen solution;

[0024] 5) Centrifuge the obtained collagen so...

Embodiment 2

[0026] A method for enzymatically extracting yak skin collagen: 1) Lay a piece of yak skin flat on the ground with the skin facing down and the quilt up, and smear 1.6kg of depilatory cream on the quilt (composed of 100 parts of water, 12 parts Sodium sulfide and 30 parts of slaked lime), stacked for 5 hours, pushed the hair, washed to get the bare hide;

[0027] 2) Crushing the obtained bare hide into leather particles with a size of 2-5mm;

[0028] 3) Add 120 parts of skin granules to the three-necked bottle, and at the same time add 300% water, 2.5% soda ash and 2% fatty acid methyl ester sodium sulfonate (MES) at the same time, at 40°C at a speed of 120r / min Stir the reaction for 3h;

[0029] 4) After rinsing the skin particles obtained in 3), add 450% water of the weight of the skin particles, 6% baking soda and 0.6% alkaline protease, stir at 40°C at a speed of 100r / min for 9 hours to obtain collagen solution;

[0030] 5) Centrifuge the obtained collagen solution at 9...

Embodiment 3

[0032] A method for enzymatically extracting yak skin collagen: 1) Spread a piece of yak skin on the ground, with the skin facing down and the quilt up, and smearing 1.2kg of depilatory cream on the quilt (composed of 100 parts of water, 11 parts Sodium sulfide and 32 parts of slaked lime), piled up for 3 hours, pushed the hair, washed to get the bare hide;

[0033] 2) Crushing the obtained bare hide into leather particles with a size of 2-5mm;

[0034] 3) Add 110 parts of skin granules to the three-necked bottle, add water with 200% weight of the skin granules, 1.3% soda ash and 3% sodium lauryl sulfate at the same time, stir and react at 35°C at a speed of 150r / min for 2h ;

[0035] 4) Rinse the skin particles obtained in 3), add 500% water of the weight of the skin particles, 5% baking soda and 0.8% alkaline protease, stir at 30°C and react at a speed of 150r / min for 10h to obtain collagen solution;

[0036] 5) Centrifuge the obtained collagen solution at 8000r / min and 4...

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PUM

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Abstract

The invention provides a method for extracting yak skin collagen by adopting an enzyme process and relates to the field of collagen extraction. The method comprises the following steps of unhairing, smashing, degreasing, enzymolysis and purification. The method for extracting the yak skin collagen by adopting the enzyme process has the advantages that yak skin is used as a raw material, and the method aims at obtaining collagen excellent in performance and improving the product value and economic benefit of the yak skin. The collagen prepared by utilizing hydrolysis of an enzymic preparation after unhairing and degreasing is high in purity, uniform in color and luster and free of extraneous odor, the extraction process is green and environment-friendly, the process is simple, a yak skin utilization range can be widened, and a yak skin utilization value can be increased.

Description

[0001] 1. Technical field [0002] The invention relates to the field of extracting collagen, in particular to a method for enzymatically extracting collagen from yak skin. [0003] 2. Background technology [0004] Collagen is a white, opaque, unbranched fibrous protein that mainly exists in animal skin, bones, tendons, etc. It is a structural protein that constitutes animal support tissues and can effectively increase the water storage function of skin tissue cells. Increase skin elasticity, keep skin soft and delicate, and also keep blood vessels and nerves in the human body tough and elastic, and have special effects on human body anti-aging and beauty. [0005] At present, the extraction methods of collagen mainly include acid method, alkali method and enzymatic method. The acid hydrolysis method is rapid and thorough, but the tryptophan will be completely destroyed, and the yield is low; the alkaline hydrolysis method will destroy amino acids containing hydroxyl and sulf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC07K14/78C12P21/06
Inventor 陈渭
Owner QINGHAI NORMAL UNIV
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