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Method for treating pollution in microalgae culture process

A technology of microalgae and pollutants, which is applied in the field of pollution control in the process of microalgae cultivation, and can solve the problems of microalgae death, secondary pollution of microalgae, difficulty in recovering growth of microalgae, etc.

Inactive Publication Date: 2017-05-24
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The pollution of protozoa during the cultivation of microalgae is inevitable. In the prior art, the pollution control of microalgae is mainly focused on the prevention process, which is only suitable for a small amount of microalgae cultivation. When large-scale microalgae is required When culturing, due to the high requirements for the pure species of microalgae and the large number of algae species, it is necessary to maintain pure species culture in all links of the entire operation process. The requirements for technology and equipment are high, which increases the operating cost and becomes a microalgae. Bottlenecks in large-scale algae farming
[0003] In the prior art, in order to carry out pure culture of microalgae, chemical drugs are usually added to the culture solution during the cultivation process. Although the direct addition of chemical drugs can kill pollutants such as protozoa or other miscellaneous algae, the The above chemical drugs also have a certain killing effect on microalgae, making it difficult for microalgae to recover in a short period of time, and even cause a large number of microalgae to die. are severely restricted in practical applications

Method used

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  • Method for treating pollution in microalgae culture process

Examples

Experimental program
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Effect test

Embodiment 1

[0076] In Example 1, Chlorella was used as the cultured algae species. During the culture process of Chlorella, under the same culture conditions, the output of Chlorella was reduced by 30%, and the culture medium was observed through a microscope to find that there was A large number of bacteria, take a certain amount of culture fluid samples, carry out optical density (OD) detection to the supernatant after filtering and removing the chlorella cells, the OD value obtained by detection is 0.2, and the density of 200,000 / ml is added to the Add flagellates to the culture solution, continue to breed, and detect the output of the chlorella and the number of the bacteria. After 2 days, the number of bacteria is significantly reduced. The chlorella cells are removed by filtration, and the supernatant is tested again for optical density. The resulting OD value was 0.1. Among them, OD represents the optical density absorbed by the detected substance.

[0077] The production of micro...

Embodiment 2

[0079] Said embodiment 2 uses Nannochloropsis pseudochloropsis as cultured algae species, in the process of cultivating Nannochloropsis pseudochloropsis, under the same cultivation conditions, the output of Nannochloropsis pseudochloropsis is reduced by 20%, and a large amount of pollution in the culture solution is found Bacteria, after filtering to remove the algae cells, the OD value of the remaining clear liquid is 0.2; add ciliates that have little influence on the growth of the Nannochloropsis algae to the culture solution at a density of 200,000 / ml, and continue to breed , and detected the output of the Nannochloropsis pseudochloropsis and the number of each species, 2 days later, the number of bacteria was significantly reduced, the clear night OD value after filtering the algae cells was 0.1, and the number of ciliates increased to 300,000 / ml.

[0080] Continue to cultivate for 2 days, the number of ciliates increases to 500,000 / ml, and the output of Nannochloropsis ps...

Embodiment 3

[0082]Described embodiment 3 uses Xanthophyllum as cultured algae, in the process of cultivating Xanthophyllum, under the same cultivation conditions, the output of Xanthophyllum is reduced by 20%, and it is found that the cyanobacteria in the culture solution are polluted by microscopic examination. Reach 1,000,000 / ml, add ciliate (because the cell wall of described Xanthophyllum is thicker, ciliate cannot prey on described Xanthophyllum) with the density of 300,000 / ml to described culture medium, culture After 3 days, the number of cyanobacteria was reduced to 300,000 / ml, and a large number of broken filaments occurred. The 300-mesh silk sieve was used to filter and continue to cultivate.

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Abstract

The invention relates to the technical field of microalgae, and in particular, relates to a method for treating pollution in a microalgae culture process. The method can effectively prevent and treat microalgae pollution, has no harm to microalgae, has no drug residues, and can improve the product quality of the microalgae. The method overcomes the problems in the prior art that chemicals have a killing effect on the microalgae, have drug residues and affect the quality of microalgae products when the chemicals are used for directly killing pollutants appearing in microalgae. The method for treating pollution in the microalgae culture process comprises the steps: step 1) inoculating a culture liquid with first microalgae, and culturing the first microalgae; step 2) detecting the culture liquid; and if pollutants are detected in the culture liquid, performing a step 3); and step 3) determining the type of pollutant species, and removing the pollutants by using a biological preventing and treating method.

Description

technical field [0001] The invention relates to the technical field of microalgae, in particular to a pollution control method in the microalgae cultivation process. Background technique [0002] The pollution of protozoa during the cultivation of microalgae is inevitable. In the prior art, the pollution control of microalgae is mainly focused on the prevention process, which is only suitable for a small amount of microalgae cultivation. When large-scale microalgae is required When culturing, due to the high requirements for the pure species of microalgae and the large number of algae species, it is necessary to maintain pure species culture in all links of the entire operation process. The requirements for technology and equipment are high, which increases the operating cost and becomes a microalgae. Bottleneck of large-scale algae cultivation. [0003] In the prior art, in order to carry out pure culture of microalgae, chemical drugs are usually added to the culture solut...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王慧岭
Owner ENN SCI & TECH DEV
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