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A kind of seawater chlorella preservation method

A seawater chlorella and preservation method technology, applied in the field of seawater chlorella preservation, can solve the problems of small number of chlorella, strict preservation conditions, and failure to meet the optimal growth of shellfish larvae, and achieve the odor of algae liquid normal effect

Active Publication Date: 2020-11-03
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of chlorella in aquaculture is becoming more and more important. Because the content of EPA and DHA in seawater chlorella is higher than that of freshwater chlorella, the application effect of chlorella in rotifers and shellfish bait is more prominent, and it is also used in fish, shrimp, It is widely used in shellfish nursery culture; however, the amount of chlorella in natural seawater is too small to meet the needs of high-density cultured fish, shrimp larvae, and shellfish larvae for optimal growth, so it is necessary to provide sufficient artificial culture and optimized , Chlorella with high nutritional value
[0003] However, the current preservation technology of seawater chlorella concentrate is relatively backward, which is mainly manifested in (1) the high-concentration algae liquid is affected by the living space, causing discomfort to the algae, and being easily infected by other pathogenic microorganisms, resulting in corruption and deterioration, which in turn affects the consumption of bait Effect; (2) The storage conditions are strict, and it needs to be stored under low-temperature refrigeration conditions, and the storage cost is high; (3) The current better fresh-keeping solution is not accepted by the market due to its high price

Method used

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  • A kind of seawater chlorella preservation method
  • A kind of seawater chlorella preservation method
  • A kind of seawater chlorella preservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Harvest and centrifuge the cultured seawater chlorella liquid, collect the algae mud, and dilute the concentration of the chlorella liquid with sterilized seawater to 10×10 8 cell;

[0013] 2. Prepare the preservative solution, the formula of the preservative solution is as follows:

[0014] Component Dosage R-polysaccharide 1g 10 2 cfu complex bacillus

1ml 10 2 cfu nitrosative bacteria

1ml 10 3 cfu nitrifying bacteria

1ml 10cfu lactic acid bacteria 1ml NH 2 CONH 2

0.1g K H 2 PO 4

0.1g

[0015] 3. After uniformly mixing 1L of chlorella concentration liquid with the above-mentioned fresh-keeping liquid, put it into a plastic bucket, seal it, and place it for observation at room temperature at 25°C, away from light and shade, and prepare 6 samples of the same specification in the same way.

[0016] 4. On the 25th day, the 26th day, the 27th day, the 28th day, the 29th day, and the 30th da...

Embodiment 2

[0018] 1. Harvest and centrifuge the cultured seawater chlorella liquid, collect the algae mud, and dilute the concentration of the chlorella liquid with sterilized seawater to 10×10 8 cell;

[0019] 2. Prepare the preservative solution, the formula of the preservative solution is as follows:

[0020]

[0021]

[0022] 3. After uniformly mixing 1L of chlorella concentration liquid with the above-mentioned fresh-keeping liquid, put it into a plastic bucket, seal it, and place it for observation at room temperature at 25°C, away from light and shade, and prepare 6 samples of the same specification in the same way.

[0023] 4. On the 25th day, the 26th day, the 27th day, the 28th day, the 29th day, and the 30th day, randomly select any one of the above-mentioned 6 samples to open, and count them under a microscope to observe the color change. The results of the changes in the odor of the liquid are shown in Table 1.

Embodiment 3

[0025] 1. Harvest and centrifuge the cultured seawater chlorella liquid, collect the algae mud, and dilute the concentration of the chlorella liquid with sterilized seawater to 10×10 8 cell;

[0026] 2. Prepare the preservative solution, the formula of the preservative solution is as follows:

[0027] Component Dosage R-polysaccharide 8g 10 2 cfu complex bacillus

7ml 10 2 cfu nitrosative bacteria

7ml 10 3 cfu nitrifying bacteria

7ml 10cfu lactic acid bacteria 7ml NH 2 CONH 2

0.5g K H 2 PO 4

0.4g

[0028] 3. After uniformly mixing 1L of chlorella concentration liquid with the above-mentioned fresh-keeping liquid, put it into a plastic bucket, seal it, and place it for observation at room temperature at 25°C, away from light and shade, and prepare 6 samples of the same specification in the same way.

[0029] 4. On the 25th day, the 26th day, the 27th day, the 28th day, the 29th day, and the 30th da...

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Abstract

The invention provides a seawater chlorella preservation method. The requirement that high-concentration seawater chlorella can be preserved for a long time at normal temperature (20 to 25 DEG C) and under the dark condition. The preservation method is economical, environmentally-friendly, and convenient and quick in operation. The method comprises the following steps: (1) preparing fresh-keeping liquid, wherein each part of fresh-keeping liquid comprises the following components: 1 to 8 g of R-polysaccharide, 1 to 7 ml of composite bacillus subtilis of 10<2> to 10<3> cfu / ml, 1 to 7 ml of nitrosobacteria of 10<2> to 10<3> cfu / ml, 1 to 7 ml of nitrobacteria of 10<2> to 10<3> cfu / ml, 1 to 7 ml of lactic acid bacteria of 10<1> to 10<2> cfu / ml, 0.1 to 0.5 g of NH2CONH2 and 0.1 to 0.4 g of KH2PO4; (2) uniformly mixing chlorella liquid which needs to be preserved and the fresh-keeping liquid, putting into a plastic barrel and performing sealed preservation, wherein l L of chlorella liquid adopts 1 part of fresh-keeping liquid.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a method for preserving seawater chlorella. Background technique [0002] Chlorella cells contain a variety of high-value nutrients and chemical raw materials, polysaccharides, proteins, pigments, etc. produced by cell metabolism. They are used in the pharmaceutical industry, food industry, animal feed, environmental testing and purification, biotechnology and renewable energy manufacturing. etc. are playing an increasingly important role. At present, the application of chlorella in aquaculture is becoming more and more important. Because the content of EPA and DHA in seawater chlorella is higher than that of freshwater chlorella, the application effect of chlorella in rotifers and shellfish bait is more prominent, and it is also used in fish, shrimp, It is widely used in shellfish nursery culture; however, the amount of chlorella in natural seawater is too small ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12N1/12C12R1/89
CPCC12N1/04C12N1/12
Inventor 云天颖袁卫
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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