Method for raising alligator pear seedlings

A cultivation method and avocado technology are applied in the directions of cultivation, seed immunization, seed and rhizome treatment, etc., and can solve the problems of inability to meet large-scale production of seedlings and low reproduction coefficient.

Active Publication Date: 2017-05-24
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the breeding process of avocado seedlings, the propagation methods such as seeding method, grafting method and high-pressure method are usually used for propagation, but these methods have low reproduction coefficients and cannot meet the needs of large-scale production for seedlings

Method used

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  • Method for raising alligator pear seedlings
  • Method for raising alligator pear seedlings
  • Method for raising alligator pear seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] After removing the avocado seeds from the ripe avocado pulp, wash the pulp attached to the outer layer of the seeds; air-dry the seeds naturally for about 3 days, rub to remove the seed coat attached to the outer surface of the seeds; before tissue culture, use a knife to remove the uneven surface of the cotyledons of the seeds Cut off the part of the seed, try to make the surface of the seed smooth and flat, so as to facilitate the sterilization of the seed surface.

[0059] To sterilize the surface of the seeds, treat them with 70% (v / v) ethanol for 1 minute, 1% (w / w) mercuric chloride for 2 minutes, and wash them with sterile water for 3 times, each time for 1 minute.

[0060] Cross-cut, cut off the top 1 / 3 of the seed, leave the remaining 2 / 3 for later use, make the first longitudinal cut, cut vertically downward along the gap between the seed cotyledons, and divide the seed into two, so that both parts retain the seed embryo part.

[0061] Use tweezers to pick up ...

Embodiment 2

[0063] After removing the avocado seeds from the ripe avocado pulp, wash the pulp attached to the outer layer of the seeds; air-dry the seeds naturally for about 3 days, rub to remove the seed coat attached to the outer surface of the seeds; before tissue culture, use a knife to remove the uneven surface of the cotyledons of the seeds Cut off the part of the seed, try to make the surface of the seed smooth and flat, so as to facilitate the sterilization of the seed surface.

[0064] To sterilize the surface of the seeds, treat them with 70% (v / v) ethanol for 2 minutes, 1% (w / w) mercuric chloride for 2 minutes, and wash them with sterile water for 3 times, each time for 1 minute.

[0065] Crosscutting, cut off the top 1 / 3 of the seed, leaving the remaining 2 / 3 for later use; the first longitudinal cut, cut vertically downward along the gap between the cotyledons of the seed, and divide the seed into two, so that both parts retain the seed The embryo part of the seed is then cut...

Embodiment 3

[0068] After removing the avocado seeds from the ripe avocado pulp, wash the pulp attached to the outer layer of the seeds; air-dry the seeds naturally for about 3 days, rub to remove the seed coat attached to the outer surface of the seeds; before tissue culture, use a knife to remove the uneven surface of the cotyledons of the seeds Cut off the part of the seed, try to make the surface of the seed smooth and flat, so as to facilitate the sterilization of the seed surface.

[0069] To sterilize the surface of the seeds, they were treated with 70% (v / v) ethanol for 1 minute, 1% (w / w) mercuric chloride for 5 minutes, and washed with sterile water for 3 times, each time for 1 minute.

[0070] Crosscutting, cut off the top 1 / 3 of the seed, leaving the remaining 2 / 3 for later use; the first longitudinal cut, cut vertically downward along the gap between the cotyledons of the seed, and divide the seed into two, so that both parts retain the seed The embryo part of the embryo, then ...

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Abstract

The invention provides a method for raising alligator pear seedlings. The method comprises the following steps: sterilizing surfaces of seeds: sequentially treating the seeds with 70% (v / v) ethanol for 0.5 to 2 minutes and 1% (w / w) mercuric chloride for 1 to 5 minutes, and carrying out sterile water washing; carrying out transection to remove tip parts of the seeds, then, stooping carrying out cutting, or continuing to carry out slitting so as to equally dividing each seed into 2 to 8 parts approximately in a manner that each part reserves the embryo part of the corresponding seed; inoculating culture media with the treated seeds, and carrying out culture. According to the method, the cut alligator pear seeds are cultured by a tissue culture method, so that a single alligator pear seed can form multiple alligator pear seedlings in one month, the aims of increasing propagation coefficient and rapidly obtaining a large number of high-quality alligator pear seedlings are achieved, and technical support and guarantee are provided for the healthy and rapid development of alligator pear industry in our country.

Description

technical field [0001] The invention relates to a method for cultivating avocado seedlings. Background technique [0002] Avocado (avocado, alligator pear) also known as avocado, scientific name Persea americana Mill., avocado of Lauraceae belongs to evergreen tree. It is native to Mexico, Ecuador, Colombia and other countries in the Americas. It is eaten as grain in Central America, and many countries in Europe and America regard it as a treasure among fruits. According to the statistics of the International Food and Agriculture Organization, avocado is widely planted in more than 50 countries around the world, and is an important agricultural and commercial fruit. [0003] The avocado fruit is pear-shaped, ellipsoidal or nearly spherical. When ripe, the peel is light green, green or dark green. After harvesting, the peel turns yellow-green, milky yellow, brown or dark brown. When ripe, the flesh is soft, milky white, Pale yellow or milky yellow, the meat is delicate, wit...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08A01G9/10
CPCA01C1/00A01C1/08A01G9/029
Inventor 程志号李淑霞孙佩光孙长君吴琼
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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