Detection method and application of chicken Salmonella enteritidis infection resistance molecular marker

Abstract

The invention relates to the technical field of gene engineering, and provides a detection method and application of a chicken Salmonella enteritidis infection resistance molecular marker-related gene LRP5. The method provides a chicken LRP5 gene rs80757564 site as the molecular marker, and correspondingly provides typing primers and corresponding PCR (polymerase chain reaction) conditions. The primers and method can be utilized to accurately detect the genotype of the LRP5 gene after the chickens are infected by Salmonella enteritidis. The individual with high Salmonella enteritidis infection resistance is selected to provide theoretical basis for chicken Salmonella-enteritidis-resistant breeding.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, and provides a detection method of a related gene LRP5, a molecular marker of Salmonella enteritidis gallinarum infection resistance, and an application thereof. Background technique [0002] Salmonella enteritidis is a Gram-negative bacillus with more than 2,500 serotypes. It can not only cause disease in livestock and poultry, but also cause serious economic losses in the breeding industry. It is also one of the main pathogens of food-borne diseases. It is one of the most frequently reported pathogenic microorganisms. Relevant reports pointed out that during 2004, 19,000 people per 1 million people in the United States were hospitalized due to Salmonella infection, and as many as 300 people died due to Salmonella infection; Disease outbreaks. [0003] Poultry and its related processed products are the main source of infection of Salmonella Enteritidis, which can enter egg products...

AI Technical Summary

Problems solved by technology

The current method of controlling Salmonella infection is mainly to use vaccines and antibiotics, but this cannot fundamentally solve the problem, and the extensive use of antibiotics will cause food safety problems and bacterial drug resistance, and lead to enhanced drug resistance of bacterial strains

With the development of molecular genetics, it is possible to improve the genetic resistance of livestock and poultry to diseases and pathogens, and to control Salmonella infection, but there is a lack of corresponding technical inspiration in the prior art

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