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Sphingobacterium sp. with aniline degradation capability and application thereof

A technology of Sphingobacter and Sphingobacter, which is applied in the field of Sphingobacter to achieve the effects of broad application prospects, high degradation rate and tolerance concentration, and safe use

Active Publication Date: 2017-05-10
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for degrading aniline wastewater by using Sphingobacterium sp.

Method used

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  • Sphingobacterium sp. with aniline degradation capability and application thereof
  • Sphingobacterium sp. with aniline degradation capability and application thereof
  • Sphingobacterium sp. with aniline degradation capability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A Sphingobacterium with the ability to degrade aniline. The 16S rDNA of Sphingobacterium is identified as belonging to the genus Sphingobacterium (Sphingobacterium sp.), and the applicant numbered it BA-4; it has been preserved in the China General Microorganisms Collection Management Center (China General Microbiological Culture Collection Center, CGMCC), Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101, Collection Number: CGMCC No.: 13428; it is short rod-shaped and belongs to Gram-negative bacteria; it forms round light yellow colonies on beef extract peptone solid medium, with moist surface and neat edges, easy to pick.

[0028] The sphingobacterium capable of degrading aniline in the present invention is derived from a sewage ditch sludge in Qufu City, Shandong Province, obtained through domestication, separation and purification of aniline, and ...

Embodiment 2

[0034] A Sphingobacterium with the ability to degrade aniline. The 16S rDNA of Sphingobacterium is identified as belonging to the genus Sphingobacterium (Sphingobacterium sp.), and the applicant numbered it BA-4; it has been preserved in the China General Microorganisms Collection Management Center (China General Microbiological Culture Collection Center, CGMCC), Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101, Collection Number: CGMCC No.: 13428; it is short rod-shaped and belongs to Gram-negative bacteria; it forms round light yellow colonies on beef extract peptone solid medium, with moist surface and neat edges, easy to pick.

[0035] The sphingobacterium capable of degrading aniline in the present invention is derived from a sewage ditch sludge in Qufu City, Shandong Province, obtained through domestication, separation and purification of aniline, and ...

Embodiment 3

[0041]A Sphingobacterium with the ability to degrade aniline. The 16S rDNA of Sphingobacterium is identified as belonging to the genus Sphingobacterium (Sphingobacterium sp.), and the applicant numbered it BA-4; it has been preserved in the China General Microorganisms Collection Management Center (China General Microbiological Culture Collection Center, CGMCC), Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101, Collection Number: CGMCC No.: 13428; it is short rod-shaped and belongs to Gram-negative bacteria; it forms round light yellow colonies on beef extract peptone solid medium, with moist surface and neat edges, easy to pick.

[0042] The sphingobacterium capable of degrading aniline in the present invention is derived from a sewage ditch sludge in Qufu City, Shandong Province, obtained through domestication, separation and purification of aniline, and c...

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Abstract

The invention discloses Sphingobacterium sp. with an aniline degradation capability. The 16 SrDNA of the Sphingobacterium sp. is identified as Sphingobacterium sp., and is numbered BA-4; the Sphingobacterium sp. is collected in the China General Microbiological Culture Collection Center with the collection number CGMCC No.:13428; the Sphingobacterium sp. is a short rod, and belongs to a Gram-negative bacterium; a round faint yellow bacterial colony is formed on a beef extract-peptone solid culture medium, and is wet in surface, neat in edge and easy to pick. The invention further discloses an application of the Sphingobacterium sp. to aniline degradation. The Sphingobacterium sp. BA-4 is applied to degradation of aniline in a sewage system, has very high degradation rate and tolerance concentration, is free from secondary pollution, is safe to use, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms and waste water treatment, and in particular relates to a sphingobacterium capable of degrading aniline and an application thereof. Background technique [0002] It is reported that the production and usage of phenol and aniline account for more than 90% of the total amount of aromatics. Among them, aniline, one of the most important intermediates in the chemical industry, is widely used: in the dye industry, it can be used to make acid bright yellow and direct orange. S, direct pink, indigo, disperse yellow brown, cationic pink FG and reactive brilliant red X-SB and other dyes can also be used to manufacture organic pigments such as golden red, scarlet pink, phenol cyanine red and oil-soluble black; in the rubber manufacturing industry, it is An important raw material for rubber additives, which can be used to manufacture anti-aging agents, accelerators, antioxidants, stabilizers, etc....

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 杨革徐庆车程川刘金锋
Owner QUFU NORMAL UNIV
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