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Application of petasites tricholobus franch extract in preparing medicines or health care foods for preventing and treating hypoxic diseases

A technology of health food and trichomoniasis, which is applied in the field of medicine

Active Publication Date: 2017-04-26
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, there have been no literature reports about the pharmacological activity of Butterbur pilaris extract and its active substances Butterbur lactones in resisting plateau hypoxia and closed hypoxia.

Method used

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  • Application of petasites tricholobus franch extract in preparing medicines or health care foods for preventing and treating hypoxic diseases
  • Application of petasites tricholobus franch extract in preparing medicines or health care foods for preventing and treating hypoxic diseases
  • Application of petasites tricholobus franch extract in preparing medicines or health care foods for preventing and treating hypoxic diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Preparation of Butterbur pilaris Extract

[0044] The preparation method steps of the butterbur extract of the present invention are as follows:

[0045] (1) extraction

[0046] After 10 kg of the rhizome of Butterbur pilaris is pulverized, use 50 L of 90% ethanol to percolate to obtain the extract according to the conventional method, and the extract is concentrated under reduced pressure and then centrifuged, and the supernatant is taken for subsequent use;

[0047] (2) Purification

[0048] The above supernatant was subjected to polystyrene type porous adsorption resin adsorption, rinsed with water and 50% aqueous ethanol to remove impurities, then eluted with 80L of 80% aqueous alcohol, and the eluate was collected.

[0049] (3) Concentration and drying

[0050] Concentrate the eluent under reduced pressure and dry it to obtain the extract of Butterbur pilaris.

Embodiment 2

[0051] Example 2. Preparation of Partial Butterbur Lactone Compounds Contained in the Butterbur pilaris Extract

[0052] Take 20 g of the butterbur extract prepared in Example 1, dissolve it in petroleum ether, perform silica gel column chromatography as usual, and use petroleum ether-acetone 50-5:1 chromatography to obtain Homofukinolide (7.9 mg), honeysuckle Butterburide-Ia (29mg), Butterburide-IIa (65mg), Butterburide-B (1.6g), Butterburide-Ⅲa (2.8g), Butterburide -IVa (2.9g), butterbur lactone-Va (50mg), butterbur lactone-D (2.6g), wherein butterbur lactone-Va is a new compound. The chemical structures of these compounds are shown in Table 1. They are butterbur lactone compounds, which were determined and analyzed by HPLC. Among them, butterbur lactone-D, butterbur lactone-B, butterbur lactone-Ⅳa and butterbur lactone The sum of the contents of the four compounds of columba lactone-Ⅲa accounts for more than 50% of the total extract, so the main components of the butterbur...

Embodiment 3

[0053] Embodiment 3. prepare butterbur lactone compounds with butterbur trichomes

[0054] 35Kg of dry leaves of Butterbur pilaris were refluxed and extracted three times with ethanol, the amount of ethanol was 175L, 70L, 35L respectively, and the extraction time was 2 hours, 1 hour, and half an hour respectively. The extract was concentrated under reduced pressure and dissolved in water to make a suspension, which was sequentially extracted with petroleum ether and ethyl acetate to obtain petroleum ether extract and ethyl acetate extract respectively. The ethyl acetate extract was eluted with a gradient of n-hexane-ethyl acetate (8:1-1:1) by silica gel column chromatography (chromatographic silica gel 200-300 mesh, Shandong Yantai Zhifu Huangwu Silica Gel Development and Experimental Factory) According to the results of TLC monitoring, the eluents were combined into thirteen parts. The fourth part was subjected to silica gel column chromatography, eluting with n-hexane:ethyl...

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Abstract

The invention relates to the technical field of medicines, in particular to a novel application of a petasites tricholobus franch extract and bakkenolide compounds contained in the petasites tricholobus franch extract in preparing medicines for preventing and treating hypoxic diseases. Results of pharmacodynamic tests show that the extract not only can significantly prolong the survival time of sealed hypoxia mice but also can obviously reduce a death rate of hypobaric hypoxia mice; and the extract can take a significant protecting effect on sealed hypoxia and plateau hypoxia; therefore, the extract can be used for preparing the medicines for preventing and treating altitude sicknesses or hypoxia symptoms such as the plateau hypoxia, the sealed hypoxia and the like.

Description

[0001] Technical field: [0002] The invention relates to the technical field of medicine, and relates to a new application of butterbur extract and butterbur lactone compounds contained therein in the preparation of drugs or health food for the prevention and treatment of hypoxia diseases, especially plateau hypoxia and airtight hypoxia diseases . [0003] Background technique: [0004] Petasites tricholobus Franch. belongs to the genus Petasites tricholobus Franch. Its rhizomes are used in the folk for detoxification and blood stasis, and for external application to treat bruises, fractures and snakebites. In order to develop and utilize the plant, the present inventor has carried out systematic chemical composition and pharmacological research on the plant, and has used four new butterbur lactone compounds and butterbur lactone compounds in Butterbur pilaris. It has applied for and obtained the Chinese invention patent ZL200610026133.1 (the authorized announcement number is...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K31/365A23L33/00A61P39/00
CPCA61K31/365A61K36/28A61P39/00
Inventor 郭美丽王业晴谢曜宇
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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