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Preparation method and application of Aedes albopictus carrying culex wolbachia

A technology of Aedes albopictus and Culex mosquito, applied in animal husbandry and other fields, can solve problems such as environmental and public health hazards, insect resistance establishment, etc., and achieve low-cost effects

Active Publication Date: 2019-07-26
GUANGZHOU WOLBAKI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a preparation method of Aedes albopictus stably carrying Culex Wolbachia and its application in the prevention and treatment of Aedes albopictus, which has the characteristics of low cost, sustainable and green environmental protection , is currently one of the most potential biological control methods for mosquito-borne diseases. It solves the problem that the use of chemical insecticides can easily make insects resistant to insecticides, and also solves the problem of harm to the environment and public health.

Method used

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  • Preparation method and application of Aedes albopictus carrying culex wolbachia
  • Preparation method and application of Aedes albopictus carrying culex wolbachia
  • Preparation method and application of Aedes albopictus carrying culex wolbachia

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Embodiment 1

[0030] 1, screening the Guangzhou local field Aedes albopictus (GUA) that tetracycline is processed obtains the Aedes albopictus that removes Wolbachia: claim that 1g tetracycline hydrochloride is dissolved in the water of 100mL and obtain concentration and be 10mg / ml tetracycline hydrochloride solution, put The obtained tetracycline hydrochloride solution with a concentration of 10 mg / ml was mixed with a sucrose solution with a mass fraction of 10% in a volume ratio of 1:10, and fed to local wild Aedes albopictus (GUA) adult mosquitoes in Guangzhou. Blood meal was provided after 7 days. Put the oviposition cup in Tianhou for it to lay eggs, and treat two generations to remove 100% of the Wolbachia in the body of the wild mosquito Aedes albopictus, and obtain Aedes albopictus without Wolbachia, referred to as Aedes albopictus GT;

[0031] 2. Preparation of Aedes albopictus that stably carries Culex pipiens (QUI) Wolbachia:

[0032] a. Using the embryo injection method to extra...

Embodiment 2

[0044] Embodiment 2: the application of the Aedes albopictus (abbreviated as GQ) stably carrying Culex mosquito Wolbachia that is obtained in Example 1 in the prevention and treatment of Aedes albopictus

[0045] Based on the background investigation of the seasonal fluctuation and population structure of Aedes albopictus populations in Shazai Island (release control area) and Xiaohu Island (control area) completed in 2014, energy release began in Shazai Island, Guangzhou in March 2015. Field field testing of GQ males (sterile males) causing cytoplasmic incompatibility with Aedes albopictus in the wild. Effective suppression depends on the mating dominance of released sterile males, which is determined by the ratio of GQ males (sterile males) to wild males. From March to the end of May, the sterility rate of ground mosquito eggs released quickly reached 70%, and the population suppression effect was obvious, but the rainy season began in June, prompting a large stock of Aedes ...

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Abstract

The invention discloses a preparation method of aedes albopictus stably carrying culex quinquefasciatus wolbachia and the application of the aedes albopictus stably carrying the culex quinquefasciatus wolbachia to prevention and control of the aedes albopictus. The preparation method comprises the following steps that Guangzhou wild aedes albopictus treated with tetracycline are screened, so that aedes albopictus without wolbachia is obtained, and the aedes albopictus stably carrying the culex quinquefasciatus wolbachia is prepared. The application of the aedes albopictus stably carrying the culex quinquefasciatus wolbachia to prevention and control of the aedes albopictus has the advantages that the cost is low, and sustainability and environment friendliness are achieved. The aedes albopictus stably carrying the culex quinquefasciatus wolbachia is one of the most potential biological measures for prevention and control of mosquito-borne diseases, the problem that insects can easily establish drug resistance under the condition of using chemical insecticides is solved, and the problem that harm to the environment and the public health is caused is also solved.

Description

[0001] Technical field: [0002] The invention relates to the field of biotechnology, in particular to a preparation method of Aedes albopictus stably carrying Culex pipiens Wolbachia and its application in preventing and treating Aedes albopictus. [0003] Background technique: [0004] Aedes albopictus, also known as the Asian tiger mosquito, is a highly aggressive mosquito and an important virus vector that can transmit a variety of pathogens, including dengue fever, Zika and other viruses. At present, dengue fever and Zika have become two important insect-borne infectious diseases worldwide, and their severity is also increasing year by year. Mosquito-borne diseases kill more than 50 million people each year, and there are currently no vaccines or specific treatments to control dengue and Zika. The traditional measure to control arboviruses is to use chemical insecticides to reduce the population of mosquitoes, which are the vectors of transmission, but they do not have a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/0339
Inventor 杨翠梁永康刘菊莲
Owner GUANGZHOU WOLBAKI BIOTECH CO LTD
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