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Colorectal-cancer joint detection evaluation method through excrement hemoglobin, transferrin and PKM2 and corresponding kit

A kit and phycoerythrin technology, which can be used in biological testing, measuring devices, material inspection products, etc., can solve the problems of missed detection and unsatisfactory reagent sensitivity.

Inactive Publication Date: 2017-03-29
SHANGHAI TELLGEN LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity of the current joint detection methods and reagents is not satisfactory, and there are a large number of missed detections.

Method used

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  • Colorectal-cancer joint detection evaluation method through excrement hemoglobin, transferrin and PKM2 and corresponding kit
  • Colorectal-cancer joint detection evaluation method through excrement hemoglobin, transferrin and PKM2 and corresponding kit
  • Colorectal-cancer joint detection evaluation method through excrement hemoglobin, transferrin and PKM2 and corresponding kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0164] Example 1: Preparation of HB, TF and PKM2 Triple Detection Kit

[0165] 1. Key material information required for the kit

[0166] The capture antibodies and detection antibodies used for hemoglobin, transferrin and PKM2 are all commercially available antibodies. The HB standard product was purchased from Meridian Company, the TF standard product was purchased from Sigma Company, and the PKM2 standard product was synthesized by Jerry Company (Shanghai).

[0167] 2. The kit of the present invention comprises the following components:

[0168] 1) Solid phase conjugate solution:

[0169] 26# microspheres (4×10 4 pc / mL), 28# microspheres coated with TF capture antibody (4×10 4 pcs / mL) and 30# microspheres (4×10 4 individual / mL), other components, 0.01M PBS (pH7.2), 0.5g / L NaN 3 .

[0170] 2) Luminescent conjugate solution:

[0171] Use phycoerythrin-labeled HB detection antibody (5ug / mL), TF detection antibody (5ug / mL) and PKM2 detection antibody (5ug / mL), 0.01M PBS (...

Embodiment 2

[0203] Example 2 HB, TF and PKM2 triple detection results of positive samples

[0204] The HB, TF and PKM2 triple detection reagent described in Example 1 was used to perform immunoassay on a total of 124 stool samples, 55 of which were from outpatients and inpatients, and were confirmed by endoscopy as colorectal cancer and progression Adenoma (a pathological state before colorectal cancer lesion) samples, 69 cases were normal control negative samples.

[0205] Among them, 55 positive samples were tested jointly, and the results are shown in Table 2.

[0206] Table 2 Screening results of positive samples for each index of the kit

[0207]

[0208] Table 3 Distribution of 55 positive samples in PKM2, HB and TF test results

[0209] Sample N 总 =55

PKM2 HB TF 26 + + + 3 + + - 2 + - - 3 + - + 5 - + + 3 - + - 8 - - + 10 - - -

[0210] It can be seen from Table 2 that the detection rates of PKM2, ...

Embodiment 3

[0212] Example 3 Analyze the sensitivity of the detection sample (n=124) with the cut-off value of the kit

[0213] Sensitivity and specificity analysis were performed on 55 positive samples confirmed as colorectal cancer or advanced adenoma and 69 normal samples.

[0214] Through the analysis and optimization of the detection data, it is found that the detection sensitivity, especially the sensitivity and accuracy of the triple detection, can be effectively improved when the cut-off values ​​shown in Table 4 below are used.

[0215] Table 4 Sensitivity analysis results of the kit for cancer sample detection

[0216] PKM2 HB TF HB+TF+PKM2 cutoff value 5ng / ml 100ng / ml 40ng / ml — sensitivity 61.8％ 67.3％ 76.4％ 81.8％

[0217] Especially when the cut-off value of TF is set to 30-50ng / ml sample (such as 40ng / ml), it can play a good role in indicating colon cancer by TF markers, which not only has high sensitivity, but also does not or is no...

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Abstract

The invention provides a colorectal-cancer joint detection evaluation kit through excrement hemoglobin, transferrin and PKM2 and a method thereof. The colorectal-cancer joint detection evaluation kit is based on a streaming fluorescence platform, and gastrointestinal tumors such as rectal cancer or colon cancer are evaluated and early diagnosed by quantitatively detecting excrement hemoglobin, transferrin and PKM2 markers.

Description

technical field [0001] The invention relates to the field of biological detection. Specifically, the invention provides a method and a corresponding kit for evaluating colorectal cancer through combined detection of fecal hemoglobin, transferrin and PKM2. Background technique [0002] Fecal occult blood test refers to the bleeding in the gastrointestinal tract that is very small, the blood is invisible to the naked eye, and a small amount of red blood cells are digested and decomposed so that they cannot be found under a microscope. A small amount of gastrointestinal bleeding in the occult blood stool does not cause a change in the color of the stool, which can only be confirmed by a fecal occult blood test. Any gastrointestinal disease that causes a small amount of bleeding may have occult blood in the stool. Therefore, occult blood examination is of great value in diagnosing a variety of gastrointestinal bleeding diseases. It is an effective means for general screening of ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/57407G01N33/57419G01N33/6893
Inventor 赵新慧蔡勇华郭安亮姚见儿
Owner SHANGHAI TELLGEN LIFE SCI CO LTD
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