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Application of SCAD gene or SCAD protein

1. SCAD, protein technology, applied in the field of biomedicine

Inactive Publication Date: 2017-03-29
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Abnormal energy metabolism of cardiomyocytes in cardiac hypertrophy has been widely reported, however, whether the energy metabolism of cardiac fibroblasts changes during myocardial fibrosis has rarely been reported

Method used

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  • Application of SCAD gene or SCAD protein
  • Application of SCAD gene or SCAD protein
  • Application of SCAD gene or SCAD protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 120

[0051] Example 1 Changes of SCAD protein, mRNA expression and enzyme activity in myocardial fibrosis tissue of 20-week-old spontaneously hypertensive rats

[0052] 1. This example tests the changes of SCAD protein, mRNA expression and enzyme activity in myocardial fibrosis tissue of 20-week-old spontaneously hypertensive rats.

[0053] 2. The result is as follows figure 1 As shown, panel A represents the results of Masson staining, cardiomyocytes are rose red, collagen fibers are blue, and the volume fraction of myocardial interstitial collagen in 20-week-old spontaneously hypertensive rats is significantly higher than that in WKY control group. Panel B represents that the protein expression of SCAD was significantly down-regulated in myocardial fibrosis tissue of 20-week-old spontaneously hypertensive rats. Panel C represents that the enzymatic activity of SCAD decreased significantly in the myocardial fibrosis tissue of 20-week-old spontaneously hypertensive rats. Panel D ...

Embodiment 2

[0055] Example 2 AngII stimulates the time-effect and dose-effect relationship of myocardial fibroblast proliferation

[0056] 1. This example tests the time-effect and dose-effect relationship of AngII stimulating the proliferation of cardiac fibroblasts.

[0057] 2. The result is as follows figure 2 As shown, graph A represents that after treating with 100nM AngII for different time (0, 6, 12, 24, 36, 48h), it was found that the increase of myocardial fibroblast activity was time-dependent, and when AngII was treated for 36h and 48h, there was no difference between the two. Significant difference. Figure B represents the time-dependent increase in the viable cell count of cardiac fibroblasts after treatment with 100 nM AngII for different times (0, 6, 12, 24, 36, 48 h). Figure C represents that after treatment with different concentrations of AngII (0.1, 1, 10, 100, 1000nM) for 36h, it was found that the activity of cardiac fibroblasts increased in a concentration-depende...

Embodiment 3

[0059] The change of the expression of embodiment 3SCAD in the myocardial fibroblast proliferation model stimulated by AngII

[0060] 1. This example tests the change of SCAD expression in AngII-stimulated myocardial fibroblast proliferation model.

[0061] 2. The result is as follows image 3 As shown, panel A represents that the expression of SCAD mRNA in cardiac fibroblasts was significantly down-regulated after being treated with 100nM AngII for different periods of time (24, 36, 48h). Panel B represents the expression of SCAD protein in cardiac fibroblasts significantly down-regulated after treatment with 100 nM AngII for different periods of time (24, 36, 48 h). Panel C represents that after treatment with different concentrations of AngII (10, 100, 1000 nM) for 36 hours, the expression of SCAD mRNA in cardiac fibroblasts was significantly down-regulated. Panel D represents that the expression of SCAD protein in cardiac fibroblasts was significantly down-regulated afte...

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Abstract

The invention discloses application of an SCAD gene or SCAD protein and particularly discloses the application of the SCAD gene and the SCAD protein or an expression accelerator or activator of the SCAD protein in preparation of medicine for preventing and treating myocardial fibrosis or medicine for preventing cardiac fibroblasts from being activated or converted into myofibroblasts or medicine for preventing cardiac fibroblast proliferation and collagen synthesis. Through establishment of animal and cell models, the important role of the SCAD in myocardial fibrosis and / or cardiac fibroblast proliferation and collagen synthesis is disclosed for the first time, a theoretical foundation and a feasible basis are provided for addition of new medicine research and development of related diseases of myocardial fibrosis and cardiac fibroblast proliferation and collagen synthesis, and the SCAD gene or the SCAD protein can be used as a new medicine effect target spot for addition of the related diseases of myocardial fibrosis and cardiac fibroblast proliferation and collagen synthesis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically, the invention relates to the application of SCAD gene or protein in the preparation of drugs for treating myocardial fibrosis, as well as in the preparation of drugs that inhibit the activation of cardiac fibroblasts and transform them into myofibroblasts, myocardial fibrosis Drug applications for fibroblast proliferation and collagen synthesis. Background technique [0002] Myocardial fibrosis is a process of imbalance between myocardial collagen synthesis and degradation metabolism. The activation and transformation of cardiac fibroblasts into myofibroblasts is the initial step of myocardial fibrosis, the proliferation of myocardial interstitial fibroblasts, and the abnormal increase in the deposition of extracellular matrix (mainly type I and type III collagen fibers), making the myocardial structure Reconstruction and function occur, which in turn affects cardiac f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K45/00A61K38/44A61P9/00
CPCA61K48/005A61K38/443A61K45/00
Inventor 周四桂徐立朋舒朝辉
Owner GUANGDONG PHARMA UNIV
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