Construction of portable glucometer-based paper analysis device for thrombin detection

A blood glucose meter, a portable technology, is applied in the field of construction of a paper analysis device for detecting thrombin based on a portable blood glucose meter, which can solve the problems of expensive instruments and complicated operations, and achieve the effects of low detection cost, high sensitivity and rapid measurement.

Inactive Publication Date: 2017-03-22
UNIV OF JINAN
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Problems solved by technology

At present, electrochemical, electrochemiluminescent, colorimetric, fluorescent and other methods have been used to detect the content of thrombin, but these traditional methods are faced with the disadvantages of complicated operation and expensive instruments.

Method used

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  • Construction of portable glucometer-based paper analysis device for thrombin detection

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Embodiment 1

[0024] Embodiment 1: the application of the paper analysis device based on the portable blood glucose meter in thrombin detection is characterized in that it comprises the following steps:

[0025] (1) Use Adobe illustrator CS4 software on the computer to design the hydrophobic wax printing pattern of the microfluidic paper chip;

[0026] (2) Print the printing pattern designed in step (1) on chromatographic paper with a wax printer, then place the printed A4 chromatographic paper in a flat heater or an oven, and heat at 100 ºC for 100 s to melt the wax And soak through the entire thickness of the paper to form a hydrophobic wall;

[0027] (3) Functionalize the hydrophilic region of the paper chip obtained in step (2): synthesize gold nanoseeds, measure 100 mL of secondary water into a single-necked flask, heat to 96 °C, and then add 1 mL of 1 wt% chlorine Auric acid solution, after reacting for 1 minute, add 3.5mL 1wt% sodium citrate solution, continue to stir for 12-18 minu...

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Abstract

The invention discloses construction of a portable glucometer-based paper analysis device for thrombin detection. A hydrophobic wax printing pattern of a microfluidic paper chip is designed by an Adobe illustrator CS4 software on a computer; gold nanoparticles grow in a hydrophilic working area of the paper chip, a hairpin-structured probe containing a thrombin aptamer chain is loaded, and a target object is captured; and a sucrose-labeled recognition chain and the hairpin structure opened probe are hybridized, glucose is generated by the catalytic hydrolysis effect of sucrose on cane sugar so as to further realize detection of thrombin through a portable glucometer.

Description

technical field [0001] The present invention relates to paper chip technology, nano material technology, DNA aptamer, portable blood glucose meter, and more specifically to the construction of a paper analysis device suitable for on-site rapid detection of non-glucose based on a portable blood glucose meter. Background technique [0002] At present, portable devices with the advantages of in-situ rapid detection, low consumption, easy operation and environmental protection have been widely used in daily life. Wherein the commercialized portable blood glucose meter has been widely used by people, but it only detects the glucose concentration in the blood of diabetic patients. The use of portable blood glucose meters combined with DNA probes to detect multiple targets has attracted more and more attention. Through the bridging effect of sucrose invertase, the portable blood glucose meter can be used for the detection of various targets. [0003] Since thrombin plays an impor...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 兰飞飞梁琳琳葛慎光于京华颜梅
Owner UNIV OF JINAN
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