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eTLC-SERS method

A platform and wetting agent technology, applied in the field of analysis and detection, to achieve the effect of broad application prospects, wide application range, and improved detection efficiency

Active Publication Date: 2017-03-22
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports on how to improve the deficiencies of traditional TLC-SERS combined technology by utilizing the various characteristics of MOFs materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In Example 1, R6G was used as the probe molecule, and the SERS activity of the eTLC detection platform was specifically detected by means of in situ detection.

[0040] To prepare the eTLC detection platform, first prepare the AuNPs@MIL-101 complex, the results are as follows figure 1 shown. figure 1 The TEM characterization diagram of the NPs@MOF structure provided in Example 1 of the present invention. Then mix the complex with silica gel for traditional thin-layer chromatography at a mass ratio of 1:1, add an appropriate amount of binder CMC-Na, stir to form a uniform homogenate, and spread it on a glass plate, overnight at room temperature, and activate After that, the eTLC detection platform is obtained.

[0041] The present invention carries out the characterization of the surface morphology of the obtained eTLC inspection platform, and the results are as follows image 3 and Figure 4 as shown, image 3 The physical picture of the eTLC detection platform pro...

Embodiment 2

[0049] In this embodiment 2, rhodamine 6G (R6G), rhodamine B (RB), and crystal violet (CV) are mixed in equal volumes at the same concentration to prepare complex system A; with complex system A as the analysis object, the present invention is used The new eTLC-SERS method, specifically explain the application method and characteristics of the eTLC-SERS method.

[0050] To prepare the eTLC detection platform, first prepare the AuNPs@MIL-101 complex, mix the complex with traditional thin-layer chromatography silica gel at a mass ratio of 1:1, add an appropriate amount of binder CMC-Na, and stir to form a uniform homogenate Afterwards, it was coated on a glass plate, left overnight at room temperature, and after activation, an eTLC detection platform was obtained.

[0051] First, follow the figure 2 As shown in the separation and detection process, the complex system A is spotted on the eTLC platform, and the spots are completely dried. Put it into the developing cylinder, us...

Embodiment 3

[0055] Mix R6G and RB with the same concentration and equal volume to prepare a complex system B; further take the complex system B as the analysis object, and use the new eTLC-SERS method of the present invention to further explain the superiority of the eTLC-SERS method over the traditional TLC-SERS method place.

[0056] Spot the complex system B on the eTLC platform, and wait until the spots are completely dry. Put it into the developing cylinder, use the developing agent to develop, after the developing is completed, take it out, and evaporate the developing agent to dry.

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Abstract

The invention relates to the field of analysis detection, and concretely relates to an enhanced thin-layer chromatography-surface enhanced Raman scattering (eTLC-SERS) analysis method. The method comprises the following steps: an eTLC platform is established; a sample solution and a HCL solution are prepared; SERS activity of the eTLC platform enables in-situ detection; an expansion process of eTLC is carried out; and Raman spectrum is collected. The invention provides a novel analysis method by combining SERS enhancement and thin-layer chromatography separation, a SERS active substrate is not required for adding while detecting, influence of coffee-ring effect in a traditional method is eliminated, detection error is reduced, detection sensitivity is obviously increased, and the operation steps are simplified. For a compound which cannot position the spot after separating, the eTLC-SERS method can be employed for detecting by using a point-by-point scanning mode, compared with the traditional method, the application scope of the eTLC-SERS method is larger, and the application prospect is wider.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular, it is a novel eTLC-SERS analysis method integrating SERS enhancement and TLC separation. Background technique [0002] Thin-layer chromatography-surface-enhanced Raman spectroscopy (TLC-SERS), using TLC to achieve simple separation of complex systems, and then using SERS to achieve qualitative and quantitative identification of the components to be detected, the two methods complement each other, simple and specific Strong, has received great attention in recent years. The analysis steps of the traditional TLC-SERS combined technique are as follows: First, after the TLC separation of the analyte, the enhanced substrate (such as gold colloid, silver colloid, etc.) is added, and then placed under a Raman instrument for detection. The reinforcing substrates currently used are mostly metal substrates, which have poor stability, low sensitivity and are not easy to store....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/95
CPCG01N30/95
Inventor 陆峰张彬彬柴逸峰史毅柳艳陈辉李皓武媚然吴棉棉陈秀娟
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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