Method for reducing macrolide resistance gene and intI1 abundance in chicken manure compost

A resistance gene and composting technology, which is applied in the field of farm solid waste treatment, can solve the problem that the impact of ARGs has not been reported, and achieve the effects of shortening the compost maturity period, easily obtaining raw materials, and facilitating degradation.

Inactive Publication Date: 2017-03-15
NORTHWEST A & F UNIV +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that surfactants can increase the number of microorganisms and enzyme activities when applied to compost, but the effect on ARGs has not been reported yet.

Method used

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  • Method for reducing macrolide resistance gene and intI1 abundance in chicken manure compost
  • Method for reducing macrolide resistance gene and intI1 abundance in chicken manure compost

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Effects of Surfactants on the Abundance of Macrolide Resistance Genes (ermB, ermF, ermX, ermQ)

[0027] Mix the fresh chicken manure with the crushed wheat straw evenly, and adjust the initial C / N ratio to about 30:1. One part uses 0.15% rhamnolipid aqueous solution (RL) to adjust the initial water content of 55% to 60%, one part uses 0.15% Tween 80 (Tw) to adjust the water content to 55% to 60%, and one part uses 0.15% The rhamnolipid and Tween 80 mixed solution (R+T) is used to adjust the water content to 55%-60%. Fully mix the stacked material and put it into a plastic foam box with a volume of 31cm×28cm×52cm and a wall thickness of 5cm. Stack it for 30 days, and turn it regularly to ensure sufficient oxygen. A total of 3 repetitions were set. Use distilled water to adjust the initial moisture content as a control.

[0028] Depend on figure 1 It can be seen that the addition of surfactants can reduce the abundance of macrolide (ermF, ermX, ermX) resist...

Embodiment 2

[0029] Example 2: Effect of surfactants on intI1 abundance

[0030] Mix fresh chicken manure with crushed wheat straw and adjust the initial C / N ratio to about 30:1. One part uses 0.15% rhamnolipid aqueous solution (RL) to adjust the initial water content of 55% to 60%, one part uses 0.15% Tween 80 (Tw) to adjust the water content to 55% to 60%, and one part uses 0.15% The rhamnolipid and Tween 80 mixed solution (R+T) is used to adjust the water content to 55%-60%. Fully mix the stacked material and put it into a plastic foam box with a volume of 31cm×28cm×52cm and a wall thickness of 5cm. Stack it for 30 days, and turn it regularly to ensure sufficient oxygen. A total of 3 repetitions were set. Use distilled water to adjust the initial moisture content to 55%-60% as a control.

[0031] Depend on figure 2 It can be seen that the addition of surfactant treatment can reduce the abundance of intI1 in chicken manure compost. Among them, the mixed addition of the two surfacta...

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Abstract

The invention provides a method for reducing macrolide resistance gene (ermB, ermF, ermX, ermQ) and intI1 abundance in chicken manure compost. The method comprises the steps of adding wheat straws in chicken manure as amendment, adding two different types of surface active agent (rhamnolipid and tween 80), mixing fresh chicken manure and crushed wheat straws in uniformity, regulating the initial C / N ratio to be roughly 30:1, adjusting the initial rate of water content of an aqueous solution using 0.15% rhamnolipid (RL) to 50-60%, adjusting the rate of water content of a solution using 0.15% tween 80 (Tw) to 55-60%, adjusting the rate of water content of a mixture solution using both 0.15% rhamnolipid and tween 80 (R+T) to 55-60%, stirring the piled materials fully and evenly, conducting the composting, using manual turning to ensure the exposure to oxygen, composting for 30 days, at the 26 day the compost being thoroughly decomposed, after the completion of the composting, the macrolide resistance gene being reduced by more than 85%, the intI1 abundance being reduced by more than 90%. The method reduces macrolide resistance gene and intI1 abundance in chicken manure compost, and has the advantages of being simple in equipment, and convenient in operation. The compost fertilizer prepared by the method has the advantages of being stable in quality, and being high in fertilizing effect.

Description

technical field [0001] The invention belongs to the technical field of farm solid waste treatment, in particular to a method for reducing the abundance of macrolide resistance genes (ermB, ermF, ermX, ermQ) and intI1 in chicken manure compost. Background technique [0002] With the development of large-scale and intensive livestock and poultry farming, antibiotics have been widely used in livestock and poultry farming as growth promoters and disease prevention drugs. However, these antibiotics are not easy to be absorbed by livestock and poultry, and only a small amount can participate in the metabolism of animals and be effectively used. Most of them will be excreted from the animal body in the form of drug prototype or metabolites with livestock and poultry feces and urine, resulting in antibiotic residues in livestock and poultry feces volume gradually increased. These untreated livestock and poultry manures with a large amount of antibiotic residues are directly applied...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F17/00
CPCC05F3/00C05F17/80C05G3/00C05F11/00Y02W30/40
Inventor 张雅君谷洁王小娟高华殷亚楠张然然张凯煜脱霞霞
Owner NORTHWEST A & F UNIV
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