Application of combination of cnidium lactone and baicalin to preparation of drugs for treating pneumonia

A technology of osthole and baicalin, which is applied in the field of medicine and can solve problems such as treatment failure and high mortality

Inactive Publication Date: 2017-03-08
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the pneumonia caused by Staphylococcus aureus infection is more serious. Under normal circumstances, people use oxacillin and cloxacillin to treat this pneumonia, but the emergence of methicillin-resistant Staphylococcus aureus (MRSA) leads to treatment failure. and higher mortality, putting greater pressure on the treatment of pneumonia

Method used

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  • Application of combination of cnidium lactone and baicalin to preparation of drugs for treating pneumonia
  • Application of combination of cnidium lactone and baicalin to preparation of drugs for treating pneumonia
  • Application of combination of cnidium lactone and baicalin to preparation of drugs for treating pneumonia

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] Hemolysis test

[0011] Staphylococcus aureus USA300 was co-cultured with different concentrations of osthole and baicalin until late logarithmic growth (OD 600 = 2.5). Take 1ml of the co-culture, centrifuge to get the supernatant; add 875μl of PBS buffer, 100μl of bacterial supernatant and 25μl of defibrillated rabbit blood to a 1.5ml centrifuge tube, mix gently and incubate at 37°C for 15min, then centrifuge to get the supernatant Clear, and measured its absorbance at 543nm (Table 1).

[0012] Table 1. The inhibitory effect of osthole and baicalin on Staphylococcus aureus α-hemolysin (%)

[0013]

[0014] Protective Test of Injured Human Lung Epithelial Cells (A549)

[0015] Staphylococcus aureus USA300 co-cultured with osthole to late logarithmic growth (OD 600nm =2.5), take 10 ml of the bacterial culture, centrifuge to get the supernatant, and filter with a 0.22 μm filter to sterilize. A549 cells were cultured in DMEM containing 10% fetal bovine serum at 2×1...

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Abstract

The invention relates to application of combination of cnidium lactone and baicalin to preparation of drugs for treating pneumonia. Rabbit erythrocyte haemolysis test, human pulmonary epithelial cell (A549) injury protection test and a mouse staphylococcus aureus pneumonia model prove that the treatment effect of the combination of cnidium lactone and baicalin is obviously better than the treatment effect of singly using each of the cnidium lactone and baicalin. Conventional antibiotics are abused and the bacterial drug resistance is increasingly enhanced; the combination of cnidium lactone and baicalin has the characteristics of high curative rate and no drug resistance, so that the combination of cnidium lactone and baicalin is capable of enhancing selectivity of application of drugs and has great significance on development of new drugs.

Description

technical field [0001] The invention relates to the application of osthole combined with baicalin in the preparation of medicines for treating pneumonia, and belongs to the field of medicine. Background technique [0002] Osthole is a main active ingredient in the fruit of the traditional Chinese medicine Cnidium - Cnidium extract, and it is a single herb commonly used in traditional Chinese medicine. Osthole has various biological activities such as warming the kidney and supporting yang, expelling wind, drying dampness, and killing insects. As a new type of natural efficacy factor, osthole has anti-tumor, anti-inflammation, anti-arrhythmia, anti-hypertension, anti-osteoporosis and other effects. Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root, which has significant biological activities, such as antibacterial, diuretic, anti-inflammatory, anti-allergic and antispasmodic effects, and has strong physiological effects such as anti-cancer response...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K31/37A61P11/00A61P31/04
CPCA61K31/7048A61K31/37
Inventor 邓旭明刘水王建锋柳博文滕梓皓张冰
Owner JILIN UNIV
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