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Lithocarpus hancei tissue culture explant obtaining method

A technology for tissue culture and acquisition method, which is applied in the directions of botanical equipment and methods, culture medium, planting substrate, etc., can solve the problems such as the difficulty of large tree sprouts to meet the needs of actual production of seedlings, the difficulty of becoming a common material for tissue culture, and the inconvenience of picking. , to achieve the effects of good explant quality, convenient mother plant management and sprout collection, and improved survival rate.

Inactive Publication Date: 2017-02-22
LIUZHOU SANNONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the large trees are located in remote forest areas, which are inconvenient to pick, difficult to germinate, small in number and difficult to sterilize. Therefore, it is difficult for large tree sprouts to meet the actual production and seedling needs.
However, isolated embryos, sterile seedlings, cotyledons and needles are used as explant materials, and the speed and quantity of adventitious buds obtained are not as fast and as many as stem segments, so it is difficult to become common materials for tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select the seeds of the excellent family of hard-shelled Ke that are free from diseases and insect pests and have full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 100-140 °C, and after natural cooling to room temperature, the seeds are sown in a 0.3% volume concentration. The potassium permanganate solution disinfected the sand bed.

[0026] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, sprayed with water and sprayed according to the situation, and apply a compound with a mass concentration of 0.5% every 7-10 days. Fertilizer solution, wherein the active ingredient of NPK in the compound fertilizer solution is more than or equal to 48%, and the sprouts will take root after 40 days.

[0027] When the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 40-60 cm, a ground diameter...

Embodiment 2

[0030] Select the seeds of the excellent family of Ke Ke with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 110°C. Sand bed disinfected with potassium manganate solution.

[0031] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 8 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0032] After the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 45-50 cm, a ground diameter of 0.3-0.4 cm, a straight trunk, and few side branches as the mother plant, and transplant them into a container cup with a size of 30 cm × 30 cm and a substrate. I...

Embodiment 3

[0035] Select the seeds of the excellent family of Ke Ke with no pests and diseases and full grains, put the seeds in an oven at 120°C after routine disinfection and soaking in hot water, and dry them in an oven at 120°C. Sand bed disinfected with potassium manganate solution.

[0036] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 9 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0037] After the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 50-55 cm, a ground diameter of 0.4-0.5 cm, a straight trunk, and few side branches as the mother plant, and transplant them into a container cup with a size of 30 ...

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Abstract

The invention discloses a Lithocarpus hancei tissue culture explant obtaining method. The method includes the steps of selecting and sowing seeds, transplanting and managing seedlings, planting and managing mother plants and obtaining explants. After superior seed sources and superior strong seedlings are selected, the mother plants are planted, and proper germinating branches are cut from the mother plants to be disinfected and inoculated. Mother plant managing and germinating branch collecting are quite convenient, the obtained explants are good in quality, low in contamination rate and death rate, high in adventitious bud germination rate, neat in germination and high in operability, the requirement for establishing a Lithocarpus hancei tissue culture rapid propagation system is met, and the method has high economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant asexual reproduction, and relates to a method for obtaining tissue culture explants of Aegypti fragrans. Background technique [0002] hard shell Ke ( Lithocarpus hancei (Benth.) Rehd ) also known as Chipigang (Taiwan), Jiuyan (Jiangxi), Bangzhang (Hunan), Shishanzhao (Guangxi), belongs to Fagaceae (Fagaceae) Lithocarpus (Lithocarpus), tree, rarely more than 15 meters high, All parts glabrous except inflorescence rachis and cupule gray pubescent. Branchlets yellowish gray or gray, often with a thin transparent waxy layer. Leaves are papery to hard leathery, ovate, obovate, broadly elliptic, obovate-elliptic, narrowly oblong-elliptic or lanceolate. The flowering period is from April to June, and the fruit matures from September to December of the following year. Bark dark gray or brownish gray, 6-10 mm thick, irregularly lobed longitudinally, peeled in small flakes, inner bark light brown, wood r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G17/00A01G9/10
CPCA01H4/001A01G17/005A01G24/00
Inventor 陈杏林
Owner LIUZHOU SANNONG TECH
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