Method for breeding and cultivating Paphiopedilum macranthum

A technology of Paphiopedilum and flower type, which is applied in the field of breeding and cultivation of Paphiopedilum, especially the large-flowered Paphiopedilum, can solve problems such as difficulty in large-scale production and utilization, low seed germination rate, complex ploidy, etc., to shorten the production cycle, The effect of eliminating the degree of browning and expanding the scale of growth

Active Publication Date: 2017-02-22
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the native species of small-flowered or multi-flowered Paphiopedilum, although the commercially-used large-flowered Paphiopedilum has much higher ornamental value, this type of Paphiopedilum is a hybrid with complex ploidy, and often only a few can be obtained. Fewer seeds are difficult to produce and utilize on a large scale, and the seed germination rate is lower than that of native species of small-flowered or multi-flowered Paphiopedilum

Method used

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  • Method for breeding and cultivating Paphiopedilum macranthum
  • Method for breeding and cultivating Paphiopedilum macranthum
  • Method for breeding and cultivating Paphiopedilum macranthum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Select healthy and mature Modi series Paphiopedilum varieties (see figure 1 Right picture), artificial cross breeding. When pollinating, the lip petals of the parents are first removed, the pollen grains of the male parent are picked out with a needle, and placed on the stigma of the female parent. Because the pollen clusters have sticky substances, the pollen grains are easy to stick to the stigma. Select pods with different maturity levels from 30 days to 300 days after pollination, and sow them on the seed germination medium respectively. The medium formula is 1 / 4MS+1mg / L6-BA+0.1mg / L NAA+1g / L peptone+25g / L banana+5.5g / L agar+1g / L activated carbon, and count the germination rate after 60 days.

[0043] Depend on figure 2 The statistical results show that there is a direct relationship between the germination rate and the degree of seed maturity of the P. The seeds on the inoculated medium did not germinate 90 days after pollination, the highest germination rate w...

Embodiment 2

[0045] Use the tissue culture seedlings of the Modi series Paphiopedilum variety described in embodiment 1, after going out of the bottle, cultivate in a 1.7-inch transparent nutrient bowl, and the substrate is bark. Set different curing humidity management conditions: the humidity is 60% and 80%. After 200 days of culture, the survival rate was counted. Test results such as image 3 As shown, when the relative humidity is 60%, the survival rate of Paphiopedilum is more than 90%, and when the relative humidity is 80%, the survival rate can reach more than 95%.

Embodiment 3

[0047] Using the Modi series Paphiopedilum variety described in Example 1, the seed germination rate test was carried out in the same manner as in Example 1, except that the following seed germination medium was used. The result is shown below.

[0048]

[0049] As can be seen from the table above, use 1 / 4MS+1mg / L 6-BA+0.1mg / L NAA+1g / L peptone+25g / L banana+5.5g / L agar+1g / L activated carbon as the seed germination medium It can significantly improve the seed germination rate of Paphiopedilum grandiflorum.

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Abstract

The invention relates to a method for breeding and cultivating Paphiopedilum macranthum. More specifically, the invention provides a method for improving the germination rate of Paphiopedilum macranthum seeds. The method comprises the following steps: step 1, artificial pollination is carried out after the Paphiopedilum macranthum blooms; and (2) the seeds are obtained from 180-240 days after pollination and are sown on a seed germination culture medium, so that buds are obtained from the seeds. The invention further provides the method for cultivating Paphiopedilum macranthum, the seed germination rate is improved by adopting the method for improving the germination rate. The method can be used for producing the Paphiopedilum macranthum with the flower diameter of 12 cm, the inflorescence is complete, the petals are intact, the ornamental effect is good, the sowing germination rate is high, the growth speed of tissue culture seedlings is high, the survival rate reaches 95% or above, and the cultivation period can be shortened to three years.

Description

technical field [0001] The invention belongs to the fields of flower cultivation and horticulture, and relates to a breeding and cultivation method of Paphiopedilum, more specifically, the invention relates to a breeding and cultivating method of Paphiopedilum, especially a large-flowered Paphiopedilum. Background technique [0002] Paphiopedilum sells well in the international flower market and is also an endangered group of Orchidaceae. Our country's apricot yellow and Paphiopediphyllum have bravely won the overall championship of the world orchid exhibition, and won nearly 100 awards in the American orchid exhibition. There are a total of 79 wild species of Paphiopedilum, about 1 / 3 of which are produced in my country. It is the only endangered wild animal and plant species in the Orchidaceae protected by the Convention on International Trade (CITES). There is a large demand gap in my country's paphiopedilum flower market, and the price is extremely expensive. The price p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02
CPCA01H1/02A01H4/001A01H4/005
Inventor 孙纪霞张英杰张京伟刘学庆郭文姣刘述河丁朋松
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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