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A kind of hydrogel for regulating three-dimensional micropatterned growth of cells and its preparation method

A micropatterning and hydrogel technology, which is applied in the field of biomedical materials and tissue engineering, can solve the problems of few surface treatment methods and limit the application of micropatterning technology in tissue engineering, and achieve a simple and easy preparation method, which is well adjustable Degradability, the effect of easy control of the reaction

Active Publication Date: 2019-03-15
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few corresponding hydrogel surface modification technologies, that is, surface treatment methods, which greatly limits the research on the interaction between hydrogels and cells and the application of tissue engineering in later stages.

Method used

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  • A kind of hydrogel for regulating three-dimensional micropatterned growth of cells and its preparation method
  • A kind of hydrogel for regulating three-dimensional micropatterned growth of cells and its preparation method
  • A kind of hydrogel for regulating three-dimensional micropatterned growth of cells and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A three-dimensional micropatterned calcium alginate hydrogel that regulates the growth of cells in straight stripes is prepared by the following method:

[0038] (1) Use the designed mask plate to prepare a striped groove first-level photoresist micropattern template with a groove width of 50 μm, a spacing of 50 μm, and a depth of 50 μm by micropattern negative lithography, and pour PDMS glue on its surface Polymer, after cross-linking and curing in an oven at 60°C for 12 hours, peel off the film to obtain a secondary PDMS glue micro-pattern template, and place the micro-pattern upward in a petri dish with a diameter of 3 cm;

[0039] (2) Prepare 20 mL of sodium alginate aqueous solution with a concentration of 10 mg / ml, add 0.06 g of calcium phosphate powder, stir for 10 min until uniformly dispersed, and obtain a mixed system of sodium alginate and calcium phosphate powder;

[0040] (3) Take 5 mL of the mixed system obtained in step (2) and pour it on the PDMS glue mi...

Embodiment 2

[0046] A three-dimensional micropatterned polyacrylamide hydrogel that regulates the growth of cell square arrays is prepared by the following method:

[0047] (1) Use the designed mask plate to prepare a first-level photoresist micropattern template with a square array of grooves with a side length of 100 μm, a spacing of 50 μm, and a depth of 100 μm by micropattern negative lithography, and pour PDMS glue on its surface Prepolymer, cross-linked and cured in an oven at 60°C for 12 hours, peeled off to obtain a secondary PDMS glue micropattern template, and placed the micropattern upward in a petri dish with a diameter of 3 cm;

[0048] (2) Mix 4mL of 50wt% acrylamide solution, 4.8mL of 0.5wt% methylenebisacrylamide solution, 1μL of tetramethylethylenediamine (TEMED) catalyst and 10% 0.1mL of ammonium persulfate (AP) solution Finally, take 5 mL and pour it on the PDMS glue micro-pattern template obtained in step (1). After the polyacrylamide hydrogel is solidified and cross-li...

Embodiment 3

[0054] A three-dimensional micropatterned gelatin hydrogel that regulates the growth of cell network stripe grooves is prepared by the following method:

[0055] (1) Use the designed mask plate to prepare a first-level photoresist micropattern template with mesh stripe grooves with a side length of 50 μm, a spacing of 100 μm, and a depth of 100 μm by micropattern negative photolithography, and pour PDMS glue on its surface Prepolymer, cross-linked and cured in an oven at 60°C for 12 hours, peeled off to obtain a secondary PDMS glue micropattern template, and placed the micropattern upward in a petri dish with a diameter of 3 cm;

[0056] (2) Disperse 5wt% gelatin evenly, take 5mL and pour it on the PDMS glue micro-pattern template obtained in step (1), place the small petri dish loaded with gelatin and PDMS glue at 4°C for 2 hours and solidify , add 5wt% glutaraldehyde for cross-linking for 1 hour, take it out, remove the PDMS glue and soak it in water several times to remove ...

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Abstract

The invention belongs to the field of biomedical materials and tissue engineering, and particularly relates to hydrogel for regulating and controlling three-dimensional micropatterning growth of cells and a preparation method thereof. The preparation method comprises the steps of preparation of micropatterning hydrogel, amination treatment of the hydrogel, treatment of the non-three-dimensional U-shaped area of the hydrogel and treatment of a three-dimensional U-shaped area of the hydrogel. The prepared hydrogel with three-dimensional micropatterns can be used for regulating and controlling three-dimensional micropatterning growth of the cells, the cells are efficiency limited to grow in the three-dimensional micropatterns, the living environment of the cells in the body is simulated well, the hydrogel is suitable for constructing a biomimetic functional tissue structure in vitro and is implanted in the body of an animal, very good biocompatibility, low inflammatory responses and good adjustable degradation capacity are shown, and the hydrogel has a certain effect on regeneration and restoration on tissue in the body of the animal and has high medical value.

Description

technical field [0001] The invention belongs to the fields of biomedical materials and tissue engineering, and specifically relates to a hydrogel for regulating three-dimensional micropatterned growth of cells and a preparation method thereof. Background technique [0002] To construct a complex of cells and biomaterial scaffolds in vitro and to study the interaction between cells and materials, one of the key points is to simulate the extracellular environment such as the morphology, structure and function of the body. The three-dimensional micropatterning of biomaterials can better simulate the living environment of cells in the body, regulate cell behavior and form bionic structures. The micropatterning of biomaterials and cell micropatterning technology is of great importance to the study of the interaction between cells and substrate materials, the interaction between cells, the regulation of topological structure on cell behavior, and the response of cells to drug stim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/12C08J3/24C08J3/075C08L5/04C08L33/26C08L89/00C08K3/32C08K5/07C08F220/56C08F222/38
CPCC08F220/56C08J3/075C08J3/24C08J7/12C08J2305/04C08J2333/26C08J2389/00C08K3/32C08K5/07C08K2003/325C08L2203/02C08L2312/00C08L5/04C08F222/385C08L89/00
Inventor 张胜民胡茜茜周旋刘浩明杨高洁
Owner HUAZHONG UNIV OF SCI & TECH
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