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A method for establishing a model of erucic acid-induced fish enteritis

A technique for establishing a method and inducing fish, which is applied in fish farming, animal husbandry, and material inspection products, can solve the problems of fish death, high mortality, and application limitations of fish enteritis models, and achieve good reproducibility and model Stable and reliable effect

Active Publication Date: 2019-03-19
SICHUAN AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In many cases, if a fish enteritis model established by an inappropriate method is used, it will easily lead to too severe lesions of fish enteritis, leading to fish death and high mortality, and the instability of the fish enteritis model, which limits the application of the fish enteritis model

Method used

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Embodiment l-6

[0028] Embodiment 1-6: the establishment method of a kind of erucic acid-induced fish enteritis model, comprises the following steps:

[0029] 1. According to the conventional method, 540 mid-growth grass carp of 230-730 g were used as the objects for establishing the enteritis model, and were randomly divided into 6 treatment groups, with 3 repetitions in each group, and 30 fish in each repetition.

[0030] 2. Preparation of experimental feed containing erucic acid

[0031] Mix erucic acid evenly in the feed whose main components are fish meal, soybean protein concentrate, rice protein powder, corn starch and α-starch, and prepare erucic acid content of 0, 4.0, 8.0, 12.0, 16.0 and 66.0 g / kg respectively The experimental feed was pelletized and stored in a 4°C refrigerator for later use;

[0032] 3. Model establishment

[0033] The grass carp selected in step 1 was raised in a cage of 1 cubic meter, and the experimental feed prepared in the above step 2 was fed with 3 wt% of...

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Abstract

The invention discloses a method for establishing an erucic acid-induced fish enteritis model, which comprises the following steps: selecting normal cultured fish as objects for establishing the enteritis model; taking experimental feed with an erucic acid content of 12 g / kg, and feeding the above-mentioned cultured fish, A fish enteritis model induced by erucic acid was obtained. The establishment method of the fish enteritis model induced by erucic acid in the present invention has the least impact on the production performance, intestinal growth and development, immune ability and muscle quality of fish, but the pathological manifestation of enteritis caused by it is the most suitable, the model is stable, reliable and reproducible it is good.

Description

technical field [0001] The invention relates to a method for establishing an animal model, in particular to a method for establishing a fish enteritis model induced by erucic acid. Background technique [0002] With the development of aquaculture and feed industry, the shortage of feed protein resources has become a common problem in the world, and it has become one of the serious restrictive factors for the development of aquaculture industry. Rapeseed is one of the main oil crops in my country, and its output accounts for more than 1 / 3 of the vegetable oil output, ranking first in the world. With the continuous improvement of rapeseed planting technology, the production has greatly developed, the yield per mu has continued to increase, and its planting range has continued to expand. Except for the main production areas in the Yangtze River Basin, Southwest and Northwest, because it can be planted in winter fallow land and does not compete with field crops, other regions a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/13A23K50/80A23K20/10
CPCA01K61/00G01N33/50A01K2207/20A01K2227/40A01K2267/0368A01K67/027
Inventor 周小秋王开卓冯琳姜维丹刘扬吴培姜俊唐旭
Owner SICHUAN AGRI UNIV
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