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A method for evaluating the salt tolerance of grass land cover seeds

A salt-tolerance and seed technology, applied in the field of plant salt-tolerance screening, can solve problems such as time-consuming, and achieve the effects of reducing labor input, spraying uniformly, and saving labor.

Active Publication Date: 2020-01-17
HEBEI UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The first object of the present invention is to provide a method for evaluating the salt tolerance of grass land cover seeds. In the method, the tubular container is used as the culture container, and the sand culture method is used to carry out the germination test, without the need for seed disinfection and arranging. The seeds are placed, and the salt solution is sprayed with a sprayer to form a set of methods for evaluating the salt tolerance of grass land cover seeds, thereby improving the technical problem that the screening method in the prior art requires a lot of materials and takes a long time at the same time, and gets rid of the need for petri dishes, cultivation Box and other conditions and restrictions on cumbersome processes

Method used

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  • A method for evaluating the salt tolerance of grass land cover seeds
  • A method for evaluating the salt tolerance of grass land cover seeds
  • A method for evaluating the salt tolerance of grass land cover seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] A method for screening salt-tolerant grassland plants, using Medicago sativa as an example, comprises the following steps:

[0122] 1. Customized culture container and culture substrate

[0123] Choose a PVC control container with a diameter of 160mm. The upper opening of the container is 110mm wide, 130mm deep, and 1100mm long. Rinse, dry and set aside.

[0124] 2. Material pretreatment

[0125] Preparation of NaCl salt solution: The mass concentration method was used to prepare NaCl salt solution, with 5 concentration gradients: 0.3%, 0.6%, 0.9%, 1.2% and 1.5%, and distilled water was set as the control group (0%).

[0126] Seed selection: select 10 alfalfa varieties, specifically: the seeds of 10 alfalfa varieties Zhongmi No.1, Algonquin, Aohan, Forerunner, Queen, WL, Debao, Suntory, Surprise, and Saidi, respectively denoted as 1 to 10 groups, see Table 1 for details.

[0127] The selected seeds are required to be bright and full in color, and every 100 seeds of ...

Embodiment 2

[0187] A method for screening salt-tolerant grassland plants, using Medicago sativa as an example, comprises the following steps:

[0188] 1. Customized culture container and culture substrate

[0189] Choose a PVC control container with a diameter of 160mm. The upper opening of the container is 110mm wide, 130mm deep, and 1100mm long. Rinse, dry and set aside.

[0190] 2. Material pretreatment

[0191] Preparation of sodium bicarbonate (NaHCO 3 ) Salt solution: prepare NaHCO by mass concentration method 3 Five concentration gradients were set up for the saline solution: 0.3%, 0.6%, 0.9%, 1.2% and 1.5%, and distilled water was set as the control group (0%).

[0192] Seed selection: select 10 alfalfa varieties, specifically: the seeds of 10 alfalfa varieties Zhongmi No.1, Algonquin, Aohan, Forerunner, Queen, WL, Debao, Suntory, Surprise, and Saidi, respectively denoted as Groups 1 to 10, see Table 1 in the above examples for details.

[0193] The selected seeds are requir...

Embodiment 3

[0224] A method for screening salt-tolerant grassland plants, using Medicago sativa as an example, comprises the following steps:

[0225] 1. Customized culture container and culture substrate

[0226] Choose a PVC control container with a diameter of 160mm. The upper opening of the container is 110mm wide, 130mm deep, and 1100mm long. Rinse, dry and set aside.

[0227] 2. Material pretreatment

[0228] Preparation of sodium carbonate (Na 2 CO 3 ) salt solution: the application of mass concentration method to prepare Na 2 CO 3 Five concentration gradients were set up for the saline solution: 0.3%, 0.6%, 0.9%, 1.2% and 1.5%, and distilled water was set as the control group (0%).

[0229] Seed selection: select 10 alfalfa varieties, specifically: the seeds of 10 alfalfa varieties Zhongmi No.1, Algonquin, Aohan, Forerunner, Queen, WL, Debao, Suntory, Surprise, and Saidi, respectively denoted as Groups 1 to 10, see Table 1 in the above examples for details.

[0230] The se...

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Abstract

The invention belongs to the field of screening for plant salt tolerance and provides a method for evaluating a salt resistance of herbaceous ground cover plant seeds. The method comprises the following steps: performing salt stress treatment on seeds sowed in a culture vessel through preparation of single-salt solutions in different concentrations in a greenhouse by taking a tubular container with an open upper part as the culture vessel; counting the number of seed germination in the period of salt stress treatment; then performing a seed germination evaluation and a salt resistance evaluation. The defects in the prior art that a screening experiment procedure is complex, a lot of time and human power are consumed and the cost is relatively high are overcome; the method can be widely used for the salt resistance evaluation of the plant seeds in a germination stage and a seedling stage.

Description

technical field [0001] The invention relates to the field of plant salt-tolerance screening, in particular to a method for evaluating the salt-tolerance of grass land cover plant seeds. Background technique [0002] At present, due to the continuous deterioration of the ecological environment and people's irrational development and utilization, the salinization of soil in arid and semi-arid areas is becoming more and more serious, which seriously restricts the development of agriculture. According to statistics, there are 100 million mu of salinized soil and more than 500 million mu of saline-alkali wasteland in my country's cultivated land, which are mainly distributed in more than a dozen provinces (regions) in the north. The area of ​​tidal flats alone accounts for 17.35% of the total coastal area; With the continuous use of chemical fertilizers and the development of irrigated agriculture, the area of ​​secondary saline-alkali land is still expanding. [0003] Grass land...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02A01C1/00G06Q50/02
CPCA01C1/00A01C1/025G06Q50/02
Inventor 刘桂霞祁永王彬彬石一均刘忠宽卢云慧张凤勤刘成宝张星
Owner HEBEI UNIVERSITY
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