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A group of InDel loci closely linked to the main anti-aphid qtl qGPAR-3-1 of mountain peach and its application

A close and linked technology, applied in the field of molecular genetics, can solve the problems of many environmental factors, long period of insect resistance identification, and difficult aggregation, and achieve the effect of saving land.

Active Publication Date: 2020-10-09
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0004] Due to the long periodicity of insect resistance identification and many environmental factors, the efficiency of conventional methods is too low, and it is more difficult to aggregate insect resistance traits with other breeding target traits

Method used

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  • A group of InDel loci closely linked to the main anti-aphid qtl qGPAR-3-1 of mountain peach and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Screening of Aphid-resistant Plants of Prunus officinale Progeny Population

[0026] 1. Collect individual leaves from the offspring of Prunus chinensis, and extract DNA from the leaves with a plant DNA extraction kit; dilute the DNA to 20ng / ul;

[0027] 2. Select the molecular marker primer corresponding to any site in the invention, and amplify its molecular marker by PCR, and select the InDel25 marker primer here. The PCR reaction system is: total volume 20μl, 1×PCR buffer, 1.5mmol / L MgCl2, 0.2mmol / L dNTPS, 0.4mmol / L primer InDel25-F and primer InDel25-R, 25ng template DNA, 1U Tag enzyme; Reaction conditions: Denaturation at 95°C for 3min, followed by 35 cycles at 94°C for 30s, 55°C for 45s, 72°C for 20s, and finally extension at 72°C for 5min.

[0028] 3. After the amplified product is separated by electrophoresis on a 6% polyacrylamide gel, check whether it contains an InDel25 resistance molecular marker with a size of 168bp (attached figure 1 ). The ...

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Abstract

The invention discloses a group of InDel sites closely linked with mountain peach aphid resistance main-effect QTL qGPAR-3-1 and an application thereof, wherein InDel 24 corresponds to the 19020017th base place, at which the base sequence is T or TGCAAT, of Scaffold 3 assembled by genome Lovell; InDel 25 corresponds to the 19031883rd base place, at which the base sequence is G or GTTAATG, of Scaffold 3 assembled by genome Lovell; InDel 26 corresponds to the 19227372nd base place, at which the base sequence is C or CTTACGTC, of Scaffold 3 assembled by genome Lovell. According to the group of InDel sites closely linked with mountain peach aphid resistance main-effect QTL qGPAR-3-1 and the application thereof, the sites and a molecular marking method can provide detection sites and efficient tools for peach aphid resistance molecule breeding, multi-character pyramiding breeding relating to aphid resistance characteristics, and screening of aphid resistance resources, thus having a broad application prospect.

Description

technical field [0001] The present invention relates to anti-green peach aphid with mountain peach ( Myzus persicae ) The InDel site closely linked to the main QTL qGPAR-3-1 and its application method belong to the field of molecular genetics, and are specially used for the molecular breeding of peach aphid resistance and the utilization of germplasm resources. Background technique [0002] Peach is native to China and has rich genetic diversity. Peach fruit is one of the bulk fruits in the world. At present, China, the United States, Italy, Spain, Greece, France and Chile are the main peach producing countries in the world. China's cultivated area and output both account for more than 50% of the world, ranking first in the world. The green peach aphid belongs to the Hemiptera Aphididae. It sucks the phloem juice of plants through the stylet. It is one of the main pests of peach trees in spring. It mainly harms the leaves, new shoots and young fruits of peach trees, causin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王新卫白翠营王力荣朱更瑞方伟超曹珂陈昌文李勇郭健丁体玉关利平张倩
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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