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A kind of prp-loaded slow-release antibiotic-mesenchymal stem cell gel system and preparation method thereof

A technology of stem cells and antibiotics, which is applied in the field of PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system and its preparation field, can solve the problem of limited types of bacteriostasis, and achieve the effect of preventing and treating open fractures and promoting healing.

Inactive Publication Date: 2019-11-19
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its antibacterial effect is generally within 24 hours, and the types of antibacterial are limited

Method used

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Embodiment 1

[0024] A PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system and its preparation method are described in detail below:

[0025] A PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system, in which thrombin and calcium ions are added to PRP to form an irregular microscopic three-dimensional grid structure of 50-200 μm, and bacteria-sensitive antibiotics are loaded on this grid structure . The grid structure is also loaded with stem cells.

[0026] A method for preparing a PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system, characterized in that: the steps are as follows:

[0027] (1) Preparation of PRP~mesenchymal stem cells: first mix 2~10ml sodium citrate anticoagulant with 10~45ml blood, prepare PRP under strict sterile conditions, put the blood mixed with anticoagulant into In the centrifuge, centrifuge at 500-3500r / min for 8-12 minutes, take out the centrifuge tube, and divide the tube into three layers, the upper layer is plat...

Embodiment 2

[0032] A PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system and its preparation method are described in detail below:

[0033] A PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system, in which thrombin and calcium ions are added to PRP to form an irregular microscopic three-dimensional grid structure of 50-200 μm, and bacteria-sensitive antibiotics are loaded on this grid structure . The grid structure is also loaded with stem cells.

[0034] A method for preparing a PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system, characterized in that: the steps are as follows:

[0035] (1) Preparation of PRP-mesenchymal stem cells: First, mix 2-10ml of sodium citrate anticoagulant with 10-45ml of blood, prepare PRP under strict aseptic conditions, and put the blood mixed with anticoagulant into In the centrifuge, centrifuge at 500-3500r / min for 8-12 minutes, take out the centrifuge tube, and divide the tube into three layers, the upper la...

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PUM

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Abstract

The invention discloses a PRP loaded slow release antibiotic-mesenchymal stem cell gel system and a preparation method thereof. Thrombin and calcium ions are added into PRP, an irregular micro three-dimensional grid structure of 50-200 micrometers is formed, and bacterium-sensitive antibiotics and stem cells are loaded on the grid structure. The PRP loaded slow release antibiotic-mesenchymal stem cell gel system and the preparation method thereof have the advantages that open fractures, bone and joint infection, skin, subcutaneous tissue and muscular tissue infection, four limb and spine implant related infection and artificial joint prosthesis peripheral infection are obviously prevented and treated, and the related infection occurrence rate is reduced. The technology releases the antibiotics within a certain time from inside to outside, bacteria around an implant are directly killed, formation of a bacterial biofilm is restrained or damaged, and the technical purpose that coalescence of bone joints and soft tissue is promoted while the antibiotics are slowly released in the body is achieved.

Description

technical field [0001] The invention belongs to the technical field of surgery and medical treatment, and specifically relates to a PRP-loaded slow-release antibiotic-mesenchymal stem cell gel system and a preparation method thereof. Background technique [0002] For a long time, bone and muscle tissue infection has always been one of the most common and serious complications in orthopedics. In 2010, it was reported in the United Kingdom that bacterial biofilm formation and progression were involved in bone and muscle tissue infection. The bacteria in the biofilm were highly Drug resistance has led to the severe situation of no available drugs in clinical practice. It can be seen that soft tissue and bone infection not only consumes huge social wealth, but also leads to serious consequences including bacterial resistance and organ function damage due to repeated long-term use of antibiotics; prolonged hospital stay, amputation and even death. The reason is that the adhesion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/56A61L27/54A61L27/52A61L27/50
CPCA61L27/3834A61L27/3847A61L27/3873A61L27/50A61L27/52A61L27/54A61L27/56A61L2300/102A61L2300/216A61L2300/252A61L2300/254A61L2300/404A61L2430/02A61L2430/30A61L2430/34
Inventor 费军余洪俊韩玉卓
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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