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LncRNA for diagnosing cerebral arterial thrombosis

An ischemic stroke, RNALOC105376505 technology, applied in the field of biomedicine, can solve problems such as unclear biological function, and achieve the effect of improving accuracy and improving survival rate

Active Publication Date: 2017-01-18
THE FIRST AFFILIATED HOSPITAL OF SHANTOU UNIV MEDICAL COLLEGE
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the prior art that microRNA plays an important role in the occurrence and development of ischemic stroke and drug resistance, but lncRNA, which is also a non-coding product, its biological function and its role in the development of ischemic stroke Not sure, needs further research
[0005] At present, abnormally expressed lncRNAs found in diseased tissues can involve various systems of the body and are widely distributed. However, due to the large number of lncRNAs, the current research on lncRNAs in the field of stroke is still in its infancy. The molecular basis of acute stroke has important implications

Method used

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  • LncRNA for diagnosing cerebral arterial thrombosis
  • LncRNA for diagnosing cerebral arterial thrombosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Screening for Gene Markers Related to Ischemic Stroke

[0040] 1. Sample collection

[0041] The blood of 10 normal people and the blood of patients with ischemic stroke were collected respectively, and the patients gave informed consent, and all the above-mentioned samples were obtained with the consent of the organizational ethics committee.

[0042] 2. Preparation of RNA samples

[0043] 1) After clinical serum samples are collected, centrifuge at 12,000 rpm for 10 minutes at high speed, repeat twice, and store the obtained serum samples at -80°C;

[0044] 2) Thaw frozen serum samples at 4°C;

[0045] 3) Pipette 250 μl serum sample to 1.5 ml EP tube, add 750 μl Trizol LS Reagent, mix by pipetting, and let stand for 5 minutes;

[0046] 4) Add chloroform (CHCl 3 : trizol 250μ1:750μ1), mix upside down, let stand for 15min;

[0047] 5) 4°C, 12000rpm, centrifuge for 15min;

[0048] 6) Carefully draw the upper liquid into a new EP tube;

[0049] 7) Add app...

Embodiment 2

[0064] Example 2 QPCR sequencing to verify differential expression of LOC105376505 gene

[0065] 1. Large-sample QPCR verification of differential expression of LOC105376505 gene. According to the sample collection method in Example 1, 100 blood samples from normal subjects and 100 patients with ischemic stroke were selected.

[0066] 2. The RNA extraction steps are the same as in Example 1.

[0067] 3. Reverse transcription:

[0068] (1) Reverse transcription reaction:

[0069] Use 25μl reaction system, take 1μg total RNA for each sample as template RNA, and add the following components to PCR tubes: DEPC water, 5× reverse transcription buffer, 10mM dNTP, 0.1mM DTT, 30μM Oligo dT, 200U / μl M-MLV, template RNA. Incubate at 42°C for 1h, then centrifuge briefly at 72°C for 10min.

[0070] (2) Primer design:

[0071] The primer sequence of the LOC105376505 gene is:

[0072] Forward primer: 5'-AACTGAAGAACCACAAGAGAAG-3'(SEQ ID NO.2)

[0073] Reverse primer: 5'-TTGCTGTCCAGGAG...

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Abstract

The invention discloses lncRNA for diagnosing cerebral arterial thrombosis. The lncRNA is LOC105376505. According to the lncRNA for diagnosing cerebral arterial thrombosis, the LOC105376505 gene is related to occurrence and development of cerebral arterial thrombosis, expression in cerebral arterial thrombosis patients is reduced, and researching of pathogenesis of cerebral arterial thrombosis is further enriched.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to an lncRNA used for the diagnosis of ischemic stroke, specifically the lncRNA is LOC105376505. Background technique [0002] Cerebrovascular disease, together with cardiovascular disease and tumor, is one of the three major diseases that seriously endanger human health. It has the characteristics of high morbidity, high disability and high mortality. One of the top three causes of death. Cerebrovascular disease is mainly a neurological disease caused by loss of local nerve function due to blood circulation disorder in brain tissue. It can be divided into acute cerebrovascular disease and chronic cerebrovascular disease. Acute cerebrovascular disease is often called stroke. In the United States, about 800,000 people suffer from stroke-like diseases every year, and the incidence rate increases with age, which brings serious consequences to the aging society. question. In China, the incide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 何文贞陈少杏陈思洽蔡德
Owner THE FIRST AFFILIATED HOSPITAL OF SHANTOU UNIV MEDICAL COLLEGE
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