Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine

A technology of ethyl guaiacol and red wine, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of quality assurance

Inactive Publication Date: 2017-01-11
CHINA AGRI UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant patents for the detection of 4-EP and 4-EG in red wine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine
  • Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine
  • Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] The present invention will be further described below by way of examples.

[0049] The present invention detects simultaneously the method for 4-ethylphenol and 4-ethylguaiacol content in red wine, and it comprises the following steps:

[0050] a. Preparation of internal standard: Accurately weigh 1mg of chromatographically pure p-cresol standard substance, dilute it with ethanol with chromatographically pure ethanol to a 1mL brown volumetric flask, store in a -20°C refrigerator, and use it as an internal standard use.

[0051] b. Headspace solid-phase microextraction: Add 10 mL of the red wine sample to be tested into a 15 mL headspace bottle equipped with a magnetic stirrer, add 100 μL of the internal standard prepared in step a and 2.5 g of NaCl. Place the headspace bottle on a magnetic heating and stirring device, pre-heat and balance at 50°C for 10 minutes, adjust the magnetic stirring speed to 850rpm, insert the extraction head, and perform headspace solid-phase ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of food detection, relates to a method for simultaneously detecting the content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine, and especially relates to a method for detecting through combination of headspace solid-phase micro-extraction-gas chromatography-mass spectrum. The invention aims to overcome the defect of the field of the present detection method for 4-EP and 4-EG in red wine and provides a method for simultaneous determination of the content of 4-EP and 4-EG in red wine by researching the optimal headspace solid-phase micro-extraction condition and the optimal gas chromatography-mass spectrum separation condition. The method mainly comprises the following steps: preparing an internal standard substance; performing headspace solid-phase micro-extraction; determining a standard curve; and performing gas chromatography-mass spectrum detection. The content of 4-EP and 4-EG in a sample can be calculated according to the obtained standard curve. Through verification, the method has the advantages of high speed, accuracy, convenience, higher precision and higher recovery rate.

Description

technical field [0001] The invention relates to the fields of food testing and food quality control, in particular to a method for determining the content of 4-ethylphenol and 4-ethylguaiacol in red wine, and in particular to a method for measuring the content of 4-ethylphenol and 4-ethylguaiacol by headspace solid-phase microextraction- Gas chromatography-mass spectrometry detection method. Background technique [0002] The smell of wine is an important indicator of wine quality and quality. A good smell will increase the complexity and coordination of wine and increase the purchase rate of consumers. Conversely, bad smells can cause wine defects. [0003] The smell of wine is composed of many substances. Among them, volatile phenols are a very important category, especially 4-ethylphenol (4-ethylphenol, 4-EP) and 4-ethylguaiacol (4-EG). [0004] 4-EP is often described as "hay shed" and "stable", while 4-EG is described as smoky and clove. At low concentration, 4-EP an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 战吉宬孙翔宇罗煊游义琳黄卫东
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com