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Calorimetric bionic competitive detection method for detecting pesticide Atrazine residue

An atrazine and detection method technology, applied in the field of biosensors, can solve problems such as lack of specificity, and achieve the effects of simple sample pretreatment, rapid specific detection method, and good application prospects

Active Publication Date: 2017-01-11
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

Due to the total exothermic heat of the thermal signal reaction, the lack of specificity of detection is the deficiency of this type of detection method

Method used

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  • Calorimetric bionic competitive detection method for detecting pesticide Atrazine residue
  • Calorimetric bionic competitive detection method for detecting pesticide Atrazine residue
  • Calorimetric bionic competitive detection method for detecting pesticide Atrazine residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Follow step 1 to prepare S-MIP.

[0053] (1) Put the surface imprinted product into the reactor and place it in the thermal regulator, and pass through the carrier liquid (0.02mol L -1 , pH 5.8 acetic acid-sodium acetate buffer, containing 2% DMF) equilibrium flow system, system flow rate 1000μl min -1 ;

[0054] (2) Prepare atrazine solution with a gradient concentration of 0-20ng mL-1 using the carrier liquid as a solvent, and mix it with a certain amount of β-lactamase-labeled atrazine to prepare a sample.

[0055] (3) After filtering through a 0.22 μm needle filter, add the sample solution. The analyte and the enzyme-labeled analyte compete to bind to the imprinted holes on the surface of the S-MIP under the mobile phase. Excess sample is washed out of the reaction column with the mobile phase.

[0056] (4) The thermal signal change produced by adding the specific substrate-ampicillin is collected by the Wheatstone bridge, converted and displayed as a peak signa...

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Abstract

The invention relates to a calorimetric bionic competitive detection method for detecting pesticide Atrazine residue, and belongs to the field of biosensors. The method is used for rapid detection of pesticide Atrazine residue in foodstuff or water source samples, and S-MIP is used as an identification element and filled in a reaction column. A competitive binding is carried out between a sample to be measured which is treated by simple pretreatment and a certain amount of beta-lactamase labeled objects to be detected in a mobile phase on blotting sites on S-MIP. Thermal signals released by adding a substrate with enzyme specificity and content of the objects to be detected are in inverse ratio. A standard curve is established based on an optimal experiment condition. The calorimetric bionic sensing method for quantitative detection of the typical pesticide Atrazine with a specific 'amplification method' has the advantages of simple pre-treatment, high sensitivity, good specificity, and quantitative detection.

Description

technical field [0001] The invention relates to a calorimetric bionic competitive detection method for detecting pesticide atrazine residues, which belongs to the field of biosensors and is used for rapidly detecting pesticide atrazine residues in food or water source samples, and has the advantages of simple sample pretreatment and high sensitivity , strong specificity, quantitative detection advantages. Background technique [0002] Herbicide Atrazine (Atrazine, ATR) is also known as atrazine, its full name is 2-chloro-4-ethylamine-6-isopropylamine-1,3,5-triazine, and its molecular weight is 215.69 Triazine herbicides As a selective systemic conduction pre-emergence and post-emergence herbicide, it achieves the purpose of removing weeds by inhibiting photosynthesis. It has been used in a large area since its development and production in the 1950s, mainly for corn, sorghum, sugarcane, and fruit trees. Dry fields such as woodlands, woodlands, etc., among which atrazine (AT...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/48C08F220/06C08F212/14C08F222/38C08F2/44C08K7/20C08J9/26
Inventor 高志贤郄志伟孙思明宁保安彭媛白家磊
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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