Portable device for nucleic acid extraction and gene amplification

Abstract

The invention relates to a portable device for nucleic acid extraction and gene amplification. The portable device comprises a nucleic acid extraction and pretreatment module and a gene amplification module matched therewith, wherein the nucleic acid extraction and pretreatment module comprises a multifunctional hot lid; four independent cavities are arranged in the multifunctional hot lid; the lower end of each cavity is connected with a valve; the value is connected with the upper end of a joint; the lower end of the joint can be connected with a reagent chamber; the bottom end of the joint is covered with a nucleic acid extraction membrane; a piston at the upper part of the cavity is tightly attached to the inside of each cavity of the multifunctional hot lid; the upper part of the piston is connected with a button; the gene amplification module comprises two warm areas capable of making a straight reciprocating motion; a supporting frame is fixed between the two warm areas; the multifunctional hot lid can be tightly combined with the supporting frame; and by driving the warm areas to make the straight reciprocating motion, the reagent chamber can relatively move between the different warm areas, thereby providing different reaction temperatures for samples and reagents in the reagent chamber and realizing gene amplification. The portable device has the advantages of convenient carrying and rapid operation.

Description

technical field [0001] The invention relates to the technical field of polymerase chain reaction (PCR), in particular to a portable device for nucleic acid extraction and gene amplification. Background technique [0002] The purpose of PCR technology is to extract the nucleic acid of the sample and achieve amplification in vitro. The amplification process of PCR refers to the specific gene amplification of the DNA polymerase in the trace biological sample under the action of the catalyst according to the characteristics of the DNA template. The whole process will include the following three parts: high temperature denaturation, low temperature annealing and medium temperature primer extension. Traditional PCR is based on three temperature changes to achieve this process, and a sample generally requires 20-40 cycles to achieve nucleic acid amplification. [0003] In terms of nucleic acid extraction, due to the complexity of the extraction method, various instruments and equ...

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Problems solved by technology

[0003] In terms of nucleic acid extraction, due to the complexity of the extraction method, various instruments and equipment are often required to complete it, such as centrifuges, pipette guns and other supporting equipment. The process is cumbersome and the equipment is expensive.

[0004] In terms of nucleic acid in vitro amplification, the traditional three temperature ranges, due to the temperature hysteresis of the instrument, the temperature change takes a long time; the sample carrier needs to switch the temperature continuously, and it needs to be equipped with Paltzer components and heat dissipation components, etc. The specific heat capacity of the temperature changing body is large, the system is limited by its own principle, and the volume of the instrument is relatively bulky

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