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Antibody composition and application thereof for detecting immunohistochemical marker protein combination of pancreatic ductal adenocarcinoma

A technology of pancreatic ductal adenocarcinoma and antibody composition, applied in the direction of immunoglobulin, protease inhibitor immunoglobulin with anti-peptide structure, measuring device, etc., can solve the problem of lack of specific pathological diagnostic markers for pancreatic ductal adenocarcinoma There are problems such as the report of sensitive markers of lesions, so as to reduce the difficulty of interpretation, overcome the low timeliness and low specificity, and simplify the operation process

Active Publication Date: 2018-02-13
韩晓
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although the above markers have been widely used in routine pathological detection of pancreatic diseases, so far, there is still a lack of specific pathological diagnostic markers for pancreatic ductal adenocarcinoma, and the differential diagnosis from other types of pancreatic cancer mainly depends on the experience of technicians
Moreover, the judgment of the prognosis of patients is mainly based on the appearance of atypical cells in the surrounding lymph nodes, and there is no report on the sensitive markers of early micrometastasis in the lesion.

Method used

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  • Antibody composition and application thereof for detecting immunohistochemical marker protein combination of pancreatic ductal adenocarcinoma
  • Antibody composition and application thereof for detecting immunohistochemical marker protein combination of pancreatic ductal adenocarcinoma
  • Antibody composition and application thereof for detecting immunohistochemical marker protein combination of pancreatic ductal adenocarcinoma

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Experimental program
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Effect test

Embodiment 1

[0033] Cell culture and immunofluorescence staining: PANC-1 cell line (purchased from ATCC, USA) was grown in DMEM medium (purchased from GIBCO) containing 10% FBS. Cells in the logarithmic phase were inoculated in a 6-well plate, and sterilized coverslips were pre-laid in the well plate, 2×10 5 / hole. After the cells grew and adhered to the wall, the fixative solution was prepared with methanol / acetone at a ratio of 1:1 for cell fixation. Wash 2-3 times with PBS and block for 1 hour at room temperature in PBS containing 5% goat serum. The primary antibody was incubated overnight at 4°C. Wash with PBS 3 times, 5 minutes each time. Fluorescently labeled goat anti-mouse or goat anti-rabbit secondary antibody was incubated at room temperature for 2 hours. Wash with PBS 3 times, 5 minutes each time. Add anti-fluorescence quenching reagent, observe and record with laser confocal microscope; see results in figure 1 .

[0034] Paraffin section specimens: 2 cases of pancreatic ...

Embodiment 2

[0038]Paraffin section specimens: from 32 patients with pancreatic diseases hospitalized in the First Affiliated Hospital of Nanjing Medical University between January 2014 and January 2015, all of which were confirmed by pathological diagnosis. The median age was 65 years, including 18 male patients and 14 female patients. The clinical data are shown in Table 1. Among them, there were 28 cases of pancreatic ductal adenocarcinoma, 1 case of mucinous cystic tumor, 1 case of neuroendocrine tumor, and 1 case of serous tumor Cystadenoma, 1 case of mucinous adenocarcinoma. Of the 28 patients with pancreatic ductal adenocarcinoma, 14 had no lymph node metastasis and 14 had lymph node metastasis.

[0039] Table 1 Basic information of 32 patients with confirmed pancreatic diseases.

[0040]

[0041] Paraffin section immunohistochemistry: select 4 μm paraffin-embedded pancreatic tissue sections, select 4 μm paraffin-embedded pancreatic tissue sections, and incubate at 60° C. for 1 ...

Embodiment 3

[0044] Paraffin section specimens: from 10 patients with pancreatic diseases hospitalized in the First Affiliated Hospital of Nanjing Medical University between January 2014 and January 2015, all of which were confirmed by pathological diagnosis. The median age was 52 years old, including 5 male patients and 5 female patients. Among them, 5 cases of pancreatic ductal adenocarcinoma, 2 cases of solid-pseudopapillary tumor, 1 case of chronic pancreatitis, 1 case of pancreatic serous cystadenoma, and 1 case of neuroendocrine tumor. No lymph node metastasis occurred in 10 cases.

[0045] Paraffin section immunohistochemistry: select 4 μm paraffin-embedded pancreatic tissue sections, select 4 μm paraffin-embedded pancreatic tissue sections, and incubate at 60° C. for 1 hour. Soak in xylene for 30 minutes to dewax, and then soak in gradient ethanol solution in turn. Block with 3% hydrogen peroxide solution in methanol for 10 minutes at room temperature. Wash with PBS 3 times, 5 m...

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Abstract

The invention discloses an antibody composition for detecting pancreatic duct adenocarcinoma immunohistochemical marker protein compositions and application of the antibody composition. The pancreatic duct adenocarcinoma immunohistochemical marker protein compositions comprise TIMP1 and CD82. The antibody composition for detecting the protein compositions comprises monoclonal antibodies of the TIMP1 and monoclonal antibodies of the CD82. The antibody composition and the application have the advantages that the TIMP1 / CD82 compositions are used as novel pancreatic duct adenocarcinoma markers, the antibody composition can be widely used in disease identification and diagnosis and prognosis monitoring of pancreatic duct adenocarcinoma, and gaps on immunohistochemical detection in the two aspects can be filled.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to an antibody composition for detecting immunohistochemical marker protein combination of pancreatic ductal adenocarcinoma and application thereof. Background technique [0002] Pancreatic cancer is the most common and serious malignant tumor in the world, known as the "king of cancer", the five-year survival rate of patients is less than 1%, and the prognosis is extremely poor. Pancreatic blood vessels and lymphatic vessels are abundant, and acini are unencapsulated, so pancreatic cancer has an insidious onset, rapid development, and is very prone to metastasis. At the same time, due to its painless growth and the absence of highly specific tumor markers, imaging techniques are still unable to detect small early-stage lesions. As a result, pancreatic cancer patients are usually diagnosed at an advanced stage. 70% of pancreatic cancers are located in the pancreatic head, pancreat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/574
CPCC07K16/2896C07K16/38G01N33/57438G01N33/57484G01N33/577
Inventor 韩晓张洁心吴倜珺詹珊珊
Owner 韩晓
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