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Plasmodium falciparum latex process detection kit

A technology of Plasmodium falciparum and detection kit, which is applied in the field of immunochromatography, can solve the problems of unsuitability for large-scale on-site investigation, misdiagnosis and missed inspection efficiency, low inspection efficiency, and achieves convenient storage and transportation, high sensitivity and simple operation. Effect

Inactive Publication Date: 2017-01-04
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional detection method is to judge by blood smear microscopy. This method is simple, economical, and has high accuracy. The disadvantage is that the judgment result is affected by factors such as the level of microscopic examination of the inspector and the degree of visual fatigue, which may easily lead to misdiagnosis and missed detection. And the inspection efficiency is not high, it is not suitable for large-scale on-site investigation and short-term mass processing of samples

Method used

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  • Plasmodium falciparum latex process detection kit
  • Plasmodium falciparum latex process detection kit
  • Plasmodium falciparum latex process detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The preparation of embodiment 1 Plasmodium falciparum latex method detection kit

[0056] 1. Reagent source:

[0057] Goat anti-rabbit IgG polyclonal antibody (purchased from MAXMED LABORATORIESINC.)

[0058] Nitrocellulose film, polyester film, polystyrene particles (purchased from SARTORIUS, a German Sartorius company)

[0059] Polystyrene particles (purchased from WHATMAN company)

[0060] 2. Preparation steps:

[0061] method 1:

[0062] 1. Preparation of sensitized latex particles:

[0063] a. Take 0.4ml of 10% carboxylated polystyrene latex into a centrifuge tube, add 1ml of pH 6.0 carbonic acid buffer solution containing 0.01% SDS 20mM to wash twice, centrifuge at 14000g / min for 10min, discard the supernatant to obtain carboxylation The polystyrene particles precipitated.

[0064] b. Suspend the carboxylated polystyrene pellet obtained in the previous step with 0.5ml of pH 6.020mM phosphate buffer solution, add 0.5ml of 10% water-soluble carbonized diimide,...

Embodiment 2

[0100] The preparation of embodiment 2 comparison kit

[0101] The preparation method of the comparison kit: the latex buffer does not contain fructose, potassium chloride and glycine, and other reagents and experimental methods are the same as methods 1-3 in Example 1.

Embodiment 3

[0102] Embodiment 3 Specificity experiment of Plasmodium falciparum latex method detection kit

[0103] Detection method:

[0104] 1. Get the Plasmodium falciparum latex method detection kit prepared in Example 1 and the comparative kit of Example 2, and place the kit on a horizontal platform.

[0105] 2. Preparation of samples to be tested: According to the four types of test objects in Table 1, whole blood was taken as samples to be tested.

[0106] 3. There are 100 subjects for each type of experiment, and the test results are shown in Table 1:

[0107] Table 1 Specificity test results of Plasmodium falciparum latex method detection kit

[0108]

[0109]

[0110] It can be seen from the above table that the Plasmodium falciparum latex method detection kit of the present invention has no cross-reaction to hepatitis B and syphilis, and has good specificity.

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Abstract

The invention relates to the field of latex process immunochromatography, and particularly discloses a plasmodium falciparum latex process detection kit. The kit comprises a reagent strip, wherein the reagent strip comprises a substrate, filtering sample paper, a sensitized latex polyester membrane, an immune nitrocellulose membrane and water absorption paper, wherein the filtering sample paper, the sensitized latex polyester membrane, the immune nitrocellulose membrane and the water absorption paper are sequentially in end-to-end connection and are fixed on the substrate; color latex particle-marked plasmodium falciparum-resistant histidine enriched protein II monoclonal antibodies I cover the sensitized latex polyester membrane; detection lines coated by plasmodium falciparum-resistant histidine enriched protein II monoclonal antibodies II and quality control lines coated with goat anti-rabbit IgG are arranged on the immune nitrocellulose membrane. The plasmodium falciparum latex process detection kit has the advantages that the response is sensitive; the detection is convenient; the detection result is stable; the cost is reduced.

Description

technical field [0001] The invention relates to the technical field of immunochromatography, in particular to a Plasmodium falciparum latex method detection kit and its preparation and application. Background technique [0002] Malaria is a vector-borne infectious disease caused by infection with Plasmodium parasites through the bite of Anopheles mosquitoes or the transfusion of the blood of a person with Plasmodium. There are four types of Plasmodium parasites in the human body, namely Plasmodium vivax, Plasmodium malariae, Plasmodium falciparum and Plasmodium ovale. In my country, mainly Plasmodium vivax and Plasmodium falciparum; the other two are rare, and some cases imported from abroad have been occasionally seen in recent years. Different Plasmodium species cause Plasmodium vivax, Plasmodium malariae, Plasmodium falciparum and Plasmodium ovale. [0003] Malaria is one of the five major parasitic diseases in the world. It has a high morbidity and mortality rate. It g...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/58G01N33/532
CPCG01N33/532G01N33/56905G01N33/585G01N2333/445Y02A50/30
Inventor 刘延龙毛远丽
Owner SHANGHAI CHEMTRON BIOTECH
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