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Primers special for identification of mating type determining genes of morchella and fruiting capability prediction method

A morel, mating type technology, applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., can solve problems such as fruiting failure and inability to complete the sexual reproduction process, so as to avoid losses , the effect of good application prospects

Active Publication Date: 2017-01-04
四川省食用菌研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two changes will cause the morel strains that originally possessed the hyphae of MAT1-1-1 and MAT1-2-1 two mating types to have only one mating type left, unable to complete the sexual reproduction process, thus leading to fruiting failure

Method used

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  • Primers special for identification of mating type determining genes of morchella and fruiting capability prediction method
  • Primers special for identification of mating type determining genes of morchella and fruiting capability prediction method
  • Primers special for identification of mating type determining genes of morchella and fruiting capability prediction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Using the method of the present invention to predict whether Morchella strains can produce mushrooms

[0045] Objective: To identify the mating type of 5 morel strains and predict whether the morel strains produced by these 5 strains can produce mushrooms.

[0046] Time: According to the PCR results, the prediction time is September 2014, and the actual fruiting time is March 2015.

[0047] Location: Xindu District, Chengdu City, Sichuan Province

[0048] Among them, A, B, and C are Morel strains owned by the inventor, and D and E are strains from two commercially available Morel strains. A and B are Morchella importuna, C and D are Morchellasextelata, and E is Morchella septimelata.

[0049] In the result table, fill in “√” if there is PCR amplification product, and fill in “×” if there is no PCR amplification product. The results are shown in Table 1.

[0050] Sampling process: aseptically pick out an appropriate amount of hyphae of the Morchella strains to be teste...

Embodiment 2

[0072] Example 2: Using the method of the present invention to predict whether Morchella species can produce mushrooms

[0073] Objective: To identify the mating type of 12 morel strains and predict whether the morel strains produced by these 12 strains can produce mushrooms.

[0074] Time: According to the PCR results, the prediction time is October 2015, and the actual fruiting time is April 2016.

[0075] Location: Aba Prefecture, Sichuan Province

[0076] Among them, M1-M8 are the strains owned by the unit, and S1-S4 are strains from four commercially available Morchella strains. M1~M4 are six sisters morel, M5~M8 are trapezium morel, S1 is seven sisters morel, S2 is trapezium morel, S3 is Morchella crassipes, S4 is Six sisters morel

[0077] See Example 1 for specific test methods.

[0078] Fill in “√” if the PCR product is amplified, and fill in “×” if it is not. In the result table, fill in "√" if there is PCR amplification product, and fill in "×" if there is no PCR amplificat...

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Abstract

The invention belongs to the technical field of molecular identification, and particularly relates to primers special for identification of mating type determining genes of morchella and a fruiting capability prediction method. The primers and the method aim at solving the technical problem that whether a morchella strain has fruiting capability or not is difficult to judge before planting. According to the technical scheme for solving the technical problem, the primers special for identification of two mating type genes of morchella are invented for the first time. By means of the primers and the method, the fruiting capability of morchella can be confirmed before cultivation, and unnecessary losses caused when morchella has no fruiting capability can be avoided.

Description

Technical field [0001] The invention belongs to the technical field of molecular identification, and specifically relates to a Morchella mating type determining gene of Morchella, a special primer for mating type identification and a method for predicting fruiting ability. Background technique [0002] Morel is a kind of delicious edible fungus in ascomycetes. Its fruit body contains 7 kinds of essential amino acids. It is sweet and cold and non-toxic, beneficial to the intestines and stomach, reducing phlegm and regulating qi. Morchella is quite rich in nutrition. According to measurements, Morchella contains 20% crude protein, 26% crude fat, 38.1% carbohydrates, and also contains a variety of amino acids, especially glutamic acid content as high as 1.76%. Therefore, some people think it is a "very good source of protein" and has the reputation of "the meat of the vegetarian". Since morels are very popular among consumers and demand exceeds supply, it is no longer possible to m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/156C12Q2531/113
Inventor 谭昊甘炳成彭卫红王波苗人云刘天海黄忠乾
Owner 四川省食用菌研究所
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