Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of quininone reductase RrQR, and application of quininone reductase RrQR in preparation of (R)-3-quinuclidinol

A technology of R-3- and quinine, which is applied in the direction of oxidoreductase, botany equipment and method, biochemical equipment and method, etc., and can solve the problem of insufficient thermal stability, etc.

Inactive Publication Date: 2017-01-04
BIOLOGY INST OF HEBEI ACAD OF SCI +1
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After 1.5 hours, 1M quinine was completely converted into R)-3-quinine alcohol, and the enantiomeric excess value (ee) was greater than 99% (patent application number: 201310422722.1); Xu Jianhe and others screened out The specific activity of quinine ketone reductase is 198U / mg, but its thermal stability is not good enough (ORGANIC LETTERS 2013Vol.15, No.194917–4919). Quinone reductase is an urgently needed technology for the production of R-3-quinine alcohol by reductase method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of quininone reductase RrQR, and application of quininone reductase RrQR in preparation of (R)-3-quinuclidinol
  • Preparation method of quininone reductase RrQR, and application of quininone reductase RrQR in preparation of (R)-3-quinuclidinol
  • Preparation method of quininone reductase RrQR, and application of quininone reductase RrQR in preparation of (R)-3-quinuclidinol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1, Recombinant quinine reductase RrQR catalyzes the asymmetric reduction of 3-quinine to produce R-3-quinine alcohol

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of quininone reductase RrQR, and applications of the quininone reductase RrQR. The applications of the quininone reductase RrQR comprise: an application of the RrQR as quininone reductase; an application of biomaterials related with the RrQR in the preparation of quininone reductase; an application of the RrQR in synthesis of R-3-quinuclidinol; and an application of the biomaterials related with the RrQR in the synthesis of the R-3-quinuclidinol. Experiments prove that the quininone reductase RrQR has high specific vitality and thermal stability and can asymmetrically reduce 3-quininone into optically active R-3-quinuclidinol.

Description

technical field [0001] The invention relates to a preparation method and application of a quinine reductase RrQR in the field of biotechnology. Background technique [0002] R-3-quinine alcohol is an important intermediate of many anticholinergic drugs, such as solifenacin, ravatorate, etc. are the latest anticholinergic drugs containing R-3-quinine alcohol structure, for the treatment of urinary incontinence and chronic obstruction Pulmonary disease (COPD) has a very good effect. There are two kinds of synthesis of R-3-quinine alcohol, chemical method and biological method. The chemical synthesis of R-3-quinine alcohol will leave metal catalysts in the product. Compared with the chemical method, the biological method has many advantages such as mild reaction conditions, high conversion rate, and strong stereoselectivity. Therefore, the biological method is developed to The synthesis of R-3-quinine alcohol is the direction of industrial production. [0003] The use of qui...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/53C12P17/18
Inventor 胡美荣马宏贾振华黄媛媛宋水山陶勇张立文
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com