Descemet's membrane endothelial corneal graft and its mechanical preparation method

A technique of Descemet's elastic layer and graft sheet, which is applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of fragility, easy to break, easy to curl, and difficult, and achieve the effect of low price, large-scale industrialization, and wide-ranging sources.

Active Publication Date: 2020-01-07
GRAND LIFE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The graft required by DMEK only includes Descemet's membrane and corneal endothelial layer, which is thin (about 15 μm), fragile, easy to break, and easy to curl, so it is difficult to completely separate it from the corneal stroma

Method used

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  • Descemet's membrane endothelial corneal graft and its mechanical preparation method
  • Descemet's membrane endothelial corneal graft and its mechanical preparation method
  • Descemet's membrane endothelial corneal graft and its mechanical preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] 1. Take the isolated porcine eyeball, and cut out the cornea with 1-2 mm scleral ring on the outer edge. see photos figure 1 (endothelial cell layer placed upwards).

[0074] 2. After completing step 1, perform trypan blue staining on the scleral sulcus (in practice, trypan blue staining can also be performed on the entire cornea obtained in step 1), then rinse with PBS buffer, and then scrape off the trabecular meshwork (pigments attached to the trabecular meshwork). See photos after trypan blue staining figure 2 (endothelial cell layer placed upwards).

[0075] 3. After completing step 2, perform trypan blue staining, tear off part or all of the trabeculae, and expose the edge of the descemet (the exposed edge of the descemet accounts for one-half of the entire edge of the descemet; in actual operation, the exposed The edge of the elastic layer accounts for more than one-fifth of the edge of the entire elastic layer, most preferably one-half). Since the trabecul...

Embodiment 2

[0087] Get the porcine DMEK graft piece that embodiment 1 prepares, detect cell density and live cell ratio.

[0088] The specific method is as follows:

[0089] 1. Take pig DMEK grafts and stain with 0.2% trypan blue staining solution for 2 minutes.

[0090] 2. After completing step 1, wash 2 times with PBS buffer.

[0091] 3. After completing step 2, stain with 1% Alizarin Red S staining solution for 3 minutes.

[0092] 4. After completing step 3, wash 2 times with PBS buffer.

[0093] 5. After step 4 is completed, observe under a microscope and count the cell density (3 high-power fields are randomly selected, and the sum is averaged) and the proportion of living cells.

[0094] Get the porcine DMEK graft that embodiment 1 prepares, all reach following standard: cell density > 2500 / mm 2 , The proportion of viable cells >90%.

[0095] The graft prepared in Example 1 was used to perform Descemet's endothelial keratoplasty on rhesus monkeys. After continuous observation f...

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Abstract

The invention discloses a graft for descemet's stripping endothelial keratoplasty and a mechanical preparation method of the graft. The preparation method of the graft comprises the following steps that a porcine cornea serves as a raw material, mechanical separation is conducted, and the graft which is composed of a descemet's membrane and an endothelium layer is obtained. According to the graft for the descemet's stripping endothelial keratoplasty and the mechanical preparation method of the graft, porcine eyeballs serve as the raw material, the graft which is composed of a descemet's membrane and an endothelium layer is prepared, the current situation that at present, human corneal endothelial donors are extremely deficient is expected to be effectively relieved, hope of sight rehabilitating is brought to a large number of patients suffering from corneal blindness, the significant social value is achieved, the very large industrial and commercial value is achieved, and the considerable economic value is brought after the graft is put on the clinical market.

Description

technical field [0001] The invention relates to a graft sheet for descemet layer corneal endothelial transplantation and its mechanical preparation method, in particular to a graft sheet for descemet layer corneal endothelial transplantation prepared from porcine cornea and its mechanical preparation method. Background technique [0002] According to statistics, there are currently about 700,000 blind patients in my country due to severe corneal diseases, and corneal blindness has become the second treatable blindness. Corneal transplantation is the only means for patients to regain sight. About 50% of patients can recover their eyesight through endothelial keratoplasty (EK). As the latest endothelial keratoplasty, Descemet Membrane Endothelial Keratoplasty (DMEK) has many advantages that other EKs do not have, so it has broad prospects for clinical application. However, the lack of human corneal donor sources seriously restricts the development of DMEK, so it is inevitabl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
CPCA61L27/3604A61L2430/16
Inventor 潘志强刘洋冯书堂
Owner GRAND LIFE SCI & TECH
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