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A Toona sinensis regeneration system based on callus differentiation

A technology for callus and callus induction, applied in plant regeneration, horticulture, horticultural methods, etc., can solve the problems of slow speed and low reproduction coefficient of toon

Inactive Publication Date: 2018-09-28
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of low reproduction coefficient and slow speed of Chinese toon in the prior art, and to provide a Chinese toon regeneration system based on callus differentiation with high reproduction coefficient and fast speed, which is beneficial to the popularization and cultivation of fine varieties

Method used

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  • A Toona sinensis regeneration system based on callus differentiation
  • A Toona sinensis regeneration system based on callus differentiation
  • A Toona sinensis regeneration system based on callus differentiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Prepare 50 L of basic medium, 10 L of induction medium, differentiation medium, and rooting medium.

[0040] 2. Acquisition of Toona sinensis aseptic explants

[0041] Toona sinensis seeds were soaked in sterile water for 6 minutes after the seed coat was removed, treated with 70% alcohol by volume under sterile conditions for 3 minutes, washed with sterile water for 4 times, and inoculated into the basic medium. The culture conditions were: humidity 70%, culture temperature light 25℃ / dark 23℃, light intensity 30 μmol photons m -2 the s -1 , under the condition of photoperiod 13 h / 11 h, after 7 days, the aseptic seedlings of Toona sinensis began to form, and after 20 days, the leaves of the aseptic plants of Toona sinensis were cut into 1.5 mm squares as explants for callus induction.

[0042] 3. Callus induction

[0043] Toona sinensis explants were placed in the induction medium at a humidity of 70%, a culture temperature of 25 / 23°C, and a light intensity of 30...

Embodiment 2

[0051] 1. Configure 50 L of basic medium, 10 L of induction medium, differentiation medium, and rooting medium.

[0052] 2. Acquisition of Toona sinensis aseptic explants

[0053] Toona sinensis seeds were soaked in sterile water for 12 h after the seed coat was removed, treated with 70% alcohol by volume under sterile conditions for 3 min, washed with sterile water for 5 times, and inoculated into the basic medium. The culture conditions were as follows: Humidity 70%, cultivation temperature light 25℃ / dark 23℃, light intensity 50 μmol photons m -2 the s -1 , under the condition of photoperiod 13 h / 11 h, after 7 days, the aseptic seedlings of Toona sinensis began to form, and after 20 days, the leaves of the aseptic plants of Toona sinensis were cut into 1.5 mm squares as explants for callus induction.

[0054] 3. Callus induction

[0055] Toona sinensis explants were placed in the induction medium at a humidity of 70%, a culture temperature of 25 / 23°C, and a light intensi...

Embodiment 3

[0063] 1. Configure 50 L of basic medium, 10 L of induction medium, differentiation medium, and rooting medium.

[0064] 2. Acquisition of Toona sinensis aseptic explants

[0065] Toona sinensis seeds were soaked in sterile water for 8 h after the seed coat was removed, treated with 70% alcohol by volume under sterile conditions for 3 min, washed with sterile water for 5 times, and inoculated into the basic medium. The culture conditions were as follows: Humidity 70%, cultivation temperature light 25°C / darkness 23°C, light intensity 40 μmol photons m -2 the s -1 , under the condition of photoperiod 13 h / 11 h, after 7 days, the aseptic seedlings of Toona sinensis began to form, and after 20 days, the leaves of the aseptic plants of Toona sinensis were cut into 1.5 mm squares as explants for callus induction.

[0066] 3. Callus induction

[0067] Toona sinensis explants were placed in the induction medium at a humidity of 70%, a culture temperature of 25 / 23°C, and a light in...

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Abstract

The invention provides a Toona sinensis Roem regenerating system based on callus differentiation. The Toona sinensis Roem regenerating system based on the callus differentiation comprises the following steps: obtaining sterile explants of Toona sinensis Roem, putting the explants into an inducing medium, culturing to form calluses, culturing the calluses in a basal medium, then transplanting into a differentiating medium, culturing and subculturing to form buds, culturing the buds in the differentiating medium, subculturing in the basal medium after the buds grow more than 4-6 leaves, then transplanting into a rooting medium, rooting to form seedlings, culturing the seedlings in the rooting medium, hardening, transplanting and the like. The Toona sinensis Roem regenerating system based on the callus differentiation is high in propagating coefficient and high in propagating speed, and helps to promote cultivation of a fine variety; the inducing rate of the calluses is 100% and the survival rate of regenerated plantlets growing from the rooted seedlings is above 96%. The Toona sinensis Roem regenerating system based on the callus differentiation can be used for rapidly propagating a precious and fine Toona sinensis Roem variety, so that large-scale production of the fine variety is achieved, needs of shed cultivation or greenhouse cultivation are met, application of the fine Toona sinensis Roem variety is accelerated; therefore, the Toona sinensis Roem regenerating system based on the callus differentiation has a relatively high market value.

Description

technical field [0001] The invention relates to a plant regeneration system, in particular to a Chinese toon regeneration system based on callus differentiation. Background technique [0002] Toon ( Toona sinensis Roem ) is a perennial tall deciduous tree belonging to the family Meliaceae. It is native to my country and has a long history of cultivation in my country. It has high economic value and has been loved by the people of our country since ancient times. The tender shoots and young leaves of Chinese toon are regarded as a good product for its unique flavor and rich nutrition. Chinese toon buds can be eaten fresh or used as raw materials for processing, and the processing and utilization of Chinese toon seed oil are also being studied. Due to the important economic value of Chinese toon, large-scale cultivation has begun in many places in my country. Toona sinensis is a highly heterozygous complex. The number of high-quality toona sinensis seeds is limited, and ser...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王友如
Owner HUBEI NORMAL UNIV
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