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A method of preparing composite amino acid chelated iron-zinc from a sea cucumber intestine nanofiltration liquid

A compound amino acid, sea cucumber sausage technology, applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry and other directions, can solve the problems of low absorption and utilization rate, discount of nutritional value, incomplete desalination, etc., and achieve low impurities and heavy metal content. , The effect of high absorption and utilization, easy absorption and utilization

Inactive Publication Date: 2016-12-21
林春梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of capsule product does not do biological treatment to sea cucumber intestines, the absorption and utilization rate is low, and the nutritional value is greatly reduced
In addition, there are still problems in the processing of raw materials: the efficiency of manual desilting of sea cucumber sausages is low, the sediment is not thorough, and the desalination is not thorough after washing with water, resulting in the problem that the content of heavy metals and salt in the product exceeds the standard.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Sea cucumber intestinal nanofiltrate prepares the method for compound amino acid chelated iron zinc, and described preparation method comprises the following steps:

[0027] (1) Pretreatment of sea cucumber intestines: collect fresh sea cucumber intestines, pretreat with pretreatment agents A and B, pretreatment agent A is a water-insoluble dextran suspension with a mass fraction of 8%, and the pretreatment agent B It is an aqueous solution of reduced glutathione with a mass fraction of 0.5%. The specific pretreatment operation is: first use pretreatment agent A to pretreat at room temperature for 2 hours, and the ratio of solid to liquid is 1:2, and then use pretreatment agent B at room temperature. Stir for pretreatment for 2 hours, then let stand for 45 minutes, the ratio of solid to liquid is 1:1;

[0028] (2) Homogenization of sea cucumber intestines: place the sea cucumber intestines pretreated in step (1) in a high-pressure homogenizer, add an appropriate amount ...

Embodiment 2

[0037] Sea cucumber intestinal nanofiltrate prepares the method for compound amino acid chelated iron zinc, and described preparation method comprises the following steps:

[0038] (1) Pretreatment of sea cucumber intestines: collect fresh sea cucumber intestines, pretreat with pretreatment agents A and B, pretreatment agent A is a water-insoluble dextran suspension with a mass fraction of 12%, and the pretreatment agent B It is an aqueous solution of reduced glutathione with a mass fraction of 1.5%. The specific pretreatment operation is: first use pretreatment agent A to pretreat at room temperature for 1 hour, and the ratio of solid to liquid is 1:3, and then use pretreatment agent B at room temperature. Stir for pretreatment for 3 hours, then let stand for 45 minutes, the ratio of solid to liquid is 1:1;

[0039] (2) Homogenization of sea cucumber intestines: place the sea cucumber intestines pretreated in step (1) in a high-pressure homogenizer, add an appropriate amount ...

Embodiment 3

[0048] Sea cucumber intestinal nanofiltrate prepares the method for compound amino acid chelated iron zinc, and described preparation method comprises the following steps:

[0049] (1) Sea cucumber intestine pretreatment: collect fresh sea cucumber intestines, and use pretreatment agents A and B to carry out pretreatment, pretreatment agent A is a water-insoluble dextran suspension with a mass fraction of 10%, and said pretreatment agent B It is an aqueous solution of reduced glutathione with a mass fraction of 1.0%. The specific pretreatment operation is: mix the pretreatment agent A and pretreatment agent B uniformly at a volume ratio of 1:1, then add fresh sea cucumber intestines, and stir at room temperature Treat for 2.5 hours, then let it stand for 1 hour, the ratio of solid to liquid is 1:2, and separate the solid and liquid for later use after the pretreatment is completed;

[0050] (2) Homogenization of sea cucumber intestines: place the sea cucumber intestines pretre...

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Abstract

The invention relates to a method of preparing composite amino acid chelated iron-zinc from a sea cucumber intestine nanofiltration liquid. The method includes (1) a step of pretreating sea cucumber intestines, (2) a step of homogenizing the sea cucumber intestines, (3) a step of first enzymatic hydrolysis, (4) a step of secondary enzymatic hydrolysis, (5) a step of ultrafiltration, (6) a step of nanofiltration, (7) a step of iron chelating, (8) a step of zinc chelating, namely a step of measuring a proper amount of a concentrate liquid, adding a proper amount of a soluble zinc salt into the concentrate liquid under stirring at 35-45 DEG C, slowly adding a proper amount of an alkali to adjust the system pH to be 7.0-7.5 with reaction time being 2-3 h, maintaining the pH value to be consistent during the reaction, cooling after the reaction is finished, centrifuging, washing solids, drying and crushing to obtain composite amino acid chelated zinc, and (9) a step of mixing, namely a step of fully mixing sea cucumber intestine polypeptide chelated iron and sea cucumber intestine polypeptide chelated zinc according to a mass ratio of 1-2:1 to obtain the composite amino acid chelated iron-zinc. The prepared composite amino acid chelated iron-zinc is rich in nutrients, easy to absorb, easy to utilize, high in absorption and utilization rate and high in nutrition value.

Description

technical field [0001] The invention relates to a method for preparing compound amino acid chelated iron and zinc. Background technique [0002] Sea cucumber is one of the precious seafood. It contains more than 50 kinds of nutrients that are beneficial to human physiological activities. Among them, the protein content is high, and the trace elements are rich in types. It also has the functions of anticoagulation, antitumor, antibacterial, antiviral and improving immunity. In recent years, there are more and more enterprises processing sea cucumbers. The nutrients contained in sea cucumber intestines, which are leftovers in the process of sea cucumber processing, are no less than those in the body wall. The vanadium content of its dry sausage is 12 parts per million, about 3 times higher than that of its body. It has the effect of warming the middle, tonifying deficiency and relieving pain, and can treat gastric and duodenal ulcers. [0003] The utilization of sea cucumbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/107
CPCC12P21/06C07K1/107
Inventor 林春梅
Owner 林春梅
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