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Application of composition in diagnosis of triple negative breast cancer

A composition and technology of breast cancer, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems of tracking, not being able to be used as the prognostic effect of breast cancer, and limited diagnostic value of breast cancer, so as to increase the sensitivity of chemotherapy Effect

Inactive Publication Date: 2016-12-07
CANCER CENT OF GUANGZHOU MEDICAL UNIV
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Problems solved by technology

Although breast cancer has a relatively good molecular classification, this molecular classification is based on breast cancer tissue detection and cannot be used as a follow-up for breast cancer prognosis
[0005] Moreover, due to the diversity of breast cancer histopathology, the heterogeneity of tumor cells with the same pathology, and the complexity and polymorphism of tumor biological behavior, the diagnostic value of single marker detection for breast cancer is limited, and it can only reflect the disease. Changes in some aspects, many scholars at home and abroad tend to screen a variety of valuable tumor markers for joint detection in order to increase the positive rate of breast cancer diagnosis
Although it has become a consensus that combined detection is better than single detection, not any combination can improve the positive rate of clinical diagnosis, and the conclusions on the best combination are quite inconsistent, and even the opposite results

Method used

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  • Application of composition in diagnosis of triple negative breast cancer
  • Application of composition in diagnosis of triple negative breast cancer
  • Application of composition in diagnosis of triple negative breast cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0028] miRNA-specific PCR detection is used, that is, cellular miRNAs are extracted and reverse-transcribed, and then detected by real-time quantitative PCR. For the detection results, see Figure 1-Figure 3 As shown, it was found that the expression levels of miR-141, miR-200a and miR-145 in triple-negative breast cancer cell lines MDA-MB-231, BT-549, SUM1315 and Hs578T were significantly lower than those in Luminal-type MCF7, T47D and BT474 cell lines, as well as HER2-overexpressing SKBR3 cell lines, and HMLE below normal mammary epithelial cells.

Embodiment 2

[0030] After the relevant tissues were taken out from the liquid nitrogen and homogenized, tissue miRNAs were extracted and reverse-transcribed, then detected by real-time quantitative PCR, and 72 pairs of breast cancer tissues paired with cancer and paracancerous normal were detected by miRNA-specific qRT-PCR The expressions of miR-141, miR-200a and miR-145 in (43 cases of Luminal type, 29 cases of triple negative type), the test results can be found in Figure 4 As shown, the results found that compared with the paired adjacent normal tissues, the expression of miR-141, miR-200a and miR-145 in triple-negative breast cancer (TNBC) tissues was significantly lower than that in Luminal breast cancer tissues.

Embodiment 3

[0032] The expression of miR-141, miR-200a and miR-145 in breast cancer tissues was detected by miRNA-specific in situ hybridization (ISH, insituhybridizations, target miRNAs stained blue, and nuclei stained red). For the detection results, see Figure 5 As shown, the results found that compared with normal breast tissue, Luminal A breast cancer tissue, Luminal B breast cancer tissue and HER2-positive breast cancer tissue, miR-141, miR-200a and miR-145 were significantly different in triple-negative breast cancer tissue. The expression was significantly down-regulated in cancer tissues.

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Abstract

The invention provides application of a composition in diagnosis of triple negative breast cancer. The composition comprises miR-141, miR-200a and miR-145. It is proved for the first time that expression of the combination of miR-141, miR-200a and miR-145 is regulated down mainly in triple negative breast cancer. Meanwhile, the content of the combination of miR-141, miR-200a and miR-145 in serum of a patient with triple negative breast cancer is obviously reduced compared with that in serum of breast cancer patients of other molecular subtyping. New data are provided for breast cancer molecular subtyping, a new target point and a serum fast detection method are provided for detection of triple negative breast cancer molecular subtyping.

Description

technical field [0001] The invention relates to a biomarker of breast cancer, in particular to the application of a composition in the diagnosis of triple negative breast cancer. Background technique [0002] Breast cancer is currently one of the most common and deadly malignant tumors in the world, and its morbidity and mortality are increasing rapidly. In order to reduce the mortality rate of breast cancer patients, in addition to reducing the incidence rate in terms of etiological prevention, improving the level of early diagnosis and the efficacy of drug treatment are two important aspects. However, the early diagnosis of lung cancer has always been a worldwide problem. At present, imaging diagnosis (B-ultrasound, mammography), chemical diagnosis (cancer response, serological and immunological indicators) and histocytological diagnosis are the three pillars of tumor diagnosis. The former has great limitations in subclinical diagnosis, while the latter two use tumor mark...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 郑国沛贺智敏张志杰李洪胜邱霓
Owner CANCER CENT OF GUANGZHOU MEDICAL UNIV
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