Method for extracting calamari health-care functional melanin

A technology for melanin and calamari, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of calamus melanin-glycoprotein structure destruction and biological activity decline, and achieve functional retention, less damage, The effect of mild reaction conditions

Inactive Publication Date: 2017-10-31
安徽阳光药业有限公司
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AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting squid health-care functional melanin, which overcomes the shortcomings of squid melanin-glycoprotein structure destruction and biological activity decline caused by existing extraction techniques

Method used

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Embodiment Construction

[0023] The method for extracting calamari health-care functional melanin comprises the following steps:

[0024] (1) Take 10kg of frozen ink sacs, thaw overnight, put them into a food chopper to crush the ink sacs, add 20L of purified water to the broken ink sacs, stir well, and use a colander to remove the unbroken tissues .

[0025] (2) Use a 200-mesh nylon net to stand and filter to remove tissue fragments and residues to obtain 22 L of crude filtrate.

[0026] (3) The crude filtrate was filtered through a mixed cellulose filter membrane with a pore size of 0.45um for the first time to obtain 22L of filtrate.

[0027] (4) The first filtrate is filtered through a mixed cellulose filter membrane with a pore size of 0.22um to obtain 22L of filtrate.

[0028] (5) Perform the first spray drying to obtain 83g of dry powder.

[0029] (6) Dissolve the dry powder with 400ml of purified water to obtain 410ml of the first-grade liquid to be separated.

[0030] (7) Use a glass colu...

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Abstract

The invention discloses a method for extracting calamary health-care melanin. The method includes the steps that firstly, calamary ink sacs are smashed and subjected to one time of rough filtering, two times of filtering through cellulose filtering membranes with different apertures and primary spraying drying to obtain dry powder; secondly, after being dissolved, the dry powder is subjected to two times of separation chromatography and spraying drying, and finally a health-care melanin product is obtained. Compared with the prior art, the adopted method is simple in process and mild in conditions, enlarged production is easy, organic solvents are not used in the production process, and the extracted melanin is complete in natural structure and fills in domestic gaps. The high-additional-value product, namely melanin, is produced by means of calamaries, so product additional value is improved, and social and economic benefits are remarkable.

Description

technical field [0001] The invention belongs to the field of natural product extraction, in particular to a new method for extracting health-care functional melanin from calamari. Background technique [0002] Calamari is a general term for the Loliginidae of the Mollusca (Cephalopoda) Teuthoidea. Also known as squid and pen tube, it was called lock tube and soft fish in ancient times. The squid family includes about 50 species, of which about 16 species have become fishing targets, such as Chinese squid (Loligo chinensis), Japanese squid (L. Squid (L. forbesi), squid (L. pealei), squid (Sepioteuthis lessoniana), etc. In China, the annual catch of squid is about 2.3 million tons, which is very huge. [0003] The ink sac is a unique structure of cephalopods, accounting for about 1.3% of its body weight. It is used to store defensive ink, which is very viscous and rich in melanin, protein and polysaccharides. In the process of production and processing, ink sacs are discar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00
CPCC09B61/00
Inventor 都剑都标张化伟
Owner 安徽阳光药业有限公司
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