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A kind of dissolvable hollow microneedle and preparation method thereof

A soluble and microneedle technology, applied in the field of microneedles, can solve the problems of non-closed hollow structures, etc., and achieve the effects of prolonging the shelf life, simple preparation method, and increasing the load capacity

Active Publication Date: 2019-06-21
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently published hollow microneedles are all made of insoluble materials, and are not closed hollow structures

Method used

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  • A kind of dissolvable hollow microneedle and preparation method thereof
  • A kind of dissolvable hollow microneedle and preparation method thereof
  • A kind of dissolvable hollow microneedle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Dissolvable hollow microneedles are prepared by the following method, the steps are:

[0042] 1. Prepare hyaluronic acid sodium salt solution: accurately weigh 80-200mg of hyaluronic acid sodium salt into a 1.5mL centrifuge tube, add 1mL dH 2 O, put it on a rotary mixer and mix it upside down for 3-7 days to mix the sodium hyaluronate evenly and use it as a high-concentration sodium hyaluronate solution for later use; at the same time, accurately weigh 50-150 mg of sodium hyaluronate into a 1.5 mL centrifuge In the tube, add 1mLdH 2 O, mix according to the above method, and use it as a low-concentration sodium hyaluronate solution for subsequent use;

[0043] 2. Take 50uL of the above-mentioned high-concentration sodium hyaluronate solution into a microneedle polydimethylsiloxane (PDMS) mold, the mold size is 1cm×1.3cm, the height of the microneedle is 50-1000μm, 4°C 1500rpm Centrifuge horizontally for 10 minutes; take out the mold and rotate it at 180 degrees and pla...

Embodiment 2

[0048] The dissolvable hollow microneedles filled with powdered medicaments are prepared by the following method, the steps are:

[0049] 1. Prepare a microneedle array with a hollow structure according to the method described in steps 1-3 in Example 1;

[0050] 2. Pour 1-2 mg of powdered medicine into the prepared hollow microneedles, centrifuge horizontally at 3000 rpm at 4°C for 10 minutes, take out the mold and rotate it 180 degrees, and continue to centrifuge for 10 minutes. Repeat this centrifugation for 3 times to make the powdered medicine enter the microneedle For the hollow part of the needle, wipe off the excess powder on the surface with a cotton swab;

[0051] 3. Add 30 μL of 5-15% (w / v) sodium hyaluronate solution on the surface of the mold, and centrifuge horizontally at 3000 rpm at 4°C for 10 minutes; take out the mold and rotate it 180 degrees, and continue to centrifuge horizontally at 3000 rpm at 4°C for 10 minutes; then take out the mold , placed in a desi...

Embodiment 3

[0054] Microneedle structure detection, detection methods and results are as follows:

[0055] 1. Slice to observe the hollow structure of the microneedle

[0056] Weigh 5 μg sulforhodamine B (SRB), add to 1mL dHO 2 In O, the sodium hyaluronate solution dyed by SRB was prepared according to the method described in step 1 of Example 1, and then the hollow microneedle patch of SRB dyed was prepared according to the method described in Example 1; the microneedle patch of SRB dyed was placed vertically Put it into liquid paraffin, and cool it quickly, then fix it on a microtome and slice it every 20 μm, and observe the microneedle structure under a microscope at any time.

[0057] Such as figure 2 As shown, after the microneedle is cut, the hollow structure can be clearly seen.

[0058] 2. Observation of hollow and powder-filled microneedles under a confocal microscope

[0059] Weigh 2 μg of fluorescein isothiocyanate (FITC), add to 1mL dHO 2 O, prepare the high-concentratio...

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Abstract

The invention discloses a soluble hollow microneedle and a preparation method thereof. The method comprises a step 1) of preparing 8-20% (w / v) of sodium hyaluronate salt solution (high concentration) to be used for preparing a needle head of the microneedle; preparing 5-15% (w / v) of sodium hyaluronate salt solution (low concentration) to be used for preparing a substrate paster of the microneedle; a step 2) of taking the high-concentration sodium hyaluronate salt solution into a microneedle die to perform horizontal centrifugation three times, so that hyaluronic acid fully enters the microneedle die to form the needle head; a step 3) of removing redundant sodium hyaluronate on the surface of the die, and forming a hollow structure microneedle array after negative pressure drying of the die; a step 4) of adding the low-concentration sodium hyaluronate salt solution on the surface of the die, performing horizontal centrifugation twice, and performing demolding after drying. The soluble hollow structure microneedle is prepared through the two-step method, the method is simple and reliable, and special devices and operation processes are not needed. The hollow microneedle can effectively deliver powdery materials into the skin.

Description

technical field [0001] The invention belongs to the field of microneedle technology, and in particular relates to a soluble hollow microneedle and a preparation method of the microneedle. The soluble hollow microneedle can effectively deliver powdered medicine into the skin. Background technique [0002] The development of painless drug delivery microneedle technology is a hot spot in recent years. The matrix materials of microneedle mainly include metal, silicon and high molecular polymer. Among them, silicon microneedles have poor biocompatibility with the human body, are expensive, brittle in texture, and complicated in process; metal materials have good mechanical strength, but general biocompatibility. In addition, silicon microneedles and metal microneedles may break when piercing and peeling off the skin. Polymer materials have good mechanical strength, simple preparation process, and can be degraded in vivo. Therefore, polymer materials are currently commonly used m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/00A61K47/36
CPCA61K9/0021A61K47/36
Inventor 陈凡严琴英
Owner HUBEI UNIV
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