Preparation method for producing acidification glycolysis feed by asynchronously fermenting mixed strains
A technology of asynchronous fermentation and bacterial fermentation, which is applied in the fields of feed, enzyme preparation and microorganism
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Embodiment 1
[0123] A preparation method for producing acidified fermented feed by mixed bacteria asynchronous fermentation, comprising the following steps:
[0124] ①. Preparation of liquid culture medium:
[0125] 100kg of corn was crushed, 200kg of water was added to adjust the slurry, and amylase was added to enzymatically hydrolyze, liquefy and saccharify to obtain 300kg of liquid culture medium with slag.
[0126] ②. Preparation of liquid lactic acid bacteria fermentation medium wet base:
[0127] Mix 0.08kg compound enzyme (xylanase: dextranase: mannanase: pectinase: the weight ratio of protease is 100:60:60:60:30) in 300kg liquid medium with slag, Insert 30kg of lactic acid bacteria seed liquid, carry out anaerobic fermentation for 48 hours under the conditions of fermentation temperature 42-45°C and initial fermentation pH 6-7, and obtain 328kg liquid lactic acid bacteria fermentation medium wet base.
[0128] ③. Preparation of liquid citric acid bacteria fermentation medium wet...
Embodiment 2
[0133] A preparation method for producing acidified fermented feed by mixed bacteria asynchronous fermentation, comprising the following steps:
[0134] ①. Preparation of solid medium:
[0135] 100kg of corn is crushed, stirred with 50kg of water, enzymatically hydrolyzed with amylase, liquefied and saccharified to obtain 150kg of saccharified solid medium.
[0136] ②. Preparation of solid lactic acid bacteria fermentation medium wet base:
[0137] Mix 0.10kg compound enzyme in 150kg saccharified solid medium (the weight ratio of xylanase:glucanase:mannanase:pectinase is 100:80:60:60), insert 50kg lactic acid bacteria The seed liquid is subjected to anaerobic fermentation for 72 hours at a fermentation temperature of 42-45° C. and an initial fermentation pH of 6-7 to obtain 185 kg of wet base of a solid lactic acid bacteria fermentation medium.
[0138] ③. Preparation of solid citric acid bacteria fermentation medium wet base:
[0139] In 185kg solid lactic acid bacteria fe...
Embodiment 3
[0144] A preparation method for producing acidified fermented feed by mixed bacteria asynchronous fermentation, comprising the following steps:
[0145] ①. Preparation of liquid culture medium:
[0146] 100kg of corn was crushed, 200kg of water was added to adjust the slurry, and amylase was added to enzymatically hydrolyze, liquefy and saccharify to obtain 300kg of liquid culture medium with slag.
[0147] ②. Preparation of liquid lactic acid bacteria fermentation medium wet base:
[0148] Mix 0.08kg compound enzyme (xylanase: dextranase: mannanase: pectinase: the weight ratio of protease is 100:60:60:60:30) in 300kg liquid medium with slag, Insert 30kg of lactic acid bacteria seed liquid, carry out anaerobic fermentation for 48 hours under the conditions of fermentation temperature 42-45°C and initial fermentation pH 6-7, and obtain 328kg liquid lactic acid bacteria fermentation medium wet base.
[0149] ③. Preparation of liquid citric acid bacteria fermentation medium wet...
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