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A method for preparing high-protein feed by fermenting citric acid cornstarch residue and mycelia residue as base material

A technology of corn starch slag and high-protein feed, applied in animal feed, animal feed, animal husbandry, etc., can solve the problems of limited protein content, difficult absorption and utilization, and high crude fiber content of citric acid slag, and the method is safe and efficient Green, limited nutritional value, simple preparation process

Active Publication Date: 2020-10-02
RIZHAO JINHE BOYUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the various nutritional components have not been extracted and separated, the citric acid slag has limited protein content and high crude fiber content, which is difficult for animals to absorb and utilize after eating. Generally, it needs to be used in conjunction with other animal feeds and the amount of addition in the feed is limited. Underutilized in the industry, the selling price has been low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Activation of strains: Inoculate the cryopreserved Candida, Bacillus subtilis and Aspergillus oryzae on the potato agar slant medium respectively, and culture at 33° C. for 28 hours.

[0032] (2) Strain expansion culture: the activated strains were respectively inoculated into Erlenmeyer flasks equipped with potato liquid medium to carry out shaker expansion culture. The culture temperature was 33° C., cultivated for 42 hours, and the shaker speed was 200 rpm, and each strain was obtained. Bacterial fluids of strains, wherein the number of viable bacteria in the bacterial fluids of Candida ruthenicum, Bacillus subtilis and Aspergillus oryzae was 3.5×10 9 cfu / mL, 5.9×10 9 cfu / mL, 2.6×10 9 cfu / mL.

[0033] (3) Preparation of solid-state fermentation medium: take citric acid cornstarch residue and mycelia residue and mix and stir evenly according to the mass ratio of 1:2.5, add a small amount of water to make the residue humidity 60%; Add 1.2% ammonium sulfate, 0.5%...

Embodiment 2

[0039] (1) Activation of strains: Inoculate the cryopreserved Candida, Bacillus subtilis and Aspergillus oryzae on the potato agar slant medium respectively, and culture at 33° C. for 28 hours.

[0040] (2) Strain expansion culture: the activated strains were respectively inoculated into Erlenmeyer flasks equipped with potato liquid medium to carry out shaker expansion culture. The culture temperature was 33° C., cultivated for 42 hours, and the shaker speed was 200 rpm, and each strain was obtained. Bacterial fluids of strains, wherein the number of viable bacteria in the bacterial fluids of Candida rutatus, Bacillus subtilis and Aspergillus oryzae was 3.6×10 9 cfu / mL, 5.5×10 9 cfu / mL, 2.8×10 9 cfu / mL.

[0041] (3) Preparation of solid-state fermentation medium: take citric acid cornstarch residue and mycelium residue and mix and stir evenly according to the mass ratio of 1:2.5, add a small amount of water to make the residue humidity 60%; use 10wt% sodium hydroxide solutio...

Embodiment 3

[0047] (1) Activation of strains: Inoculate the cryopreserved Candida, Bacillus subtilis and Aspergillus oryzae on the potato agar slant medium respectively, and culture at 30° C. for 32 hours.

[0048] (2) Strain expansion culture: the activated strains were respectively inoculated into Erlenmeyer flasks equipped with potato liquid medium to carry out shaker expansion culture. The culture temperature was 30°C, cultivated for 48 hours, and the shaker speed was 250rpm, and each strain was obtained. Bacterial fluids of strains, wherein the number of viable bacteria in the bacterial fluids of Candida ruthenicum, Bacillus subtilis and Aspergillus oryzae was 3.0×10 9 cfu / mL, 5.8×10 9 cfu / mL, 2.3×10 9 cfu / mL.

[0049] (3) Preparation of solid-state fermentation medium: take citric acid cornstarch residue and mycelia residue according to the mass ratio of 1:4, mix and stir evenly, add a small amount of water to make the residue humidity 65%. Use 10wt% sodium hydroxide solution to ...

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Abstract

The invention relates to a method for preparing high-protein feedstuff by fermenting citric acid cornstarch residue and mycelium residue as base material through mixed bacteria. The cornstarch residue and the mycelium residue produced by citric acid fermentation are used as solid medium, the compound enzyme and the mixed bacteria are added to carry out solid state fermentation, and the products after fermentation are dried and crushed to produce high protein feed products. The method utilizes the biological fermentation technology to efficiently utilize the cornstarch residue and the mycelium residue in the citric acid production, thereby improving the utilization value of the citric acid fermentation residue and making it become a new protein feedstuff with high added value. The fermented high-protein feedstaff is rich in protein, organic acid, amino acids, vitamins, minerals, trace elements, biological enzymes and peptides, small peptides and so on enhancing immunity of animals, which have high nutritive value. With nutrition, attractant and prebiotics and other functions, animal digestion and absorption of animals are facilitated, animal digestive function is improved, and animal immunity is enhanced.

Description

technical field [0001] The invention relates to the field of fermentation of citric acid dregs, in particular to a method for preparing high-protein feed by fermenting citric acid cornstarch dregs and mycelia dregs as base materials. Background technique [0002] my country is a big country in the production of citric acid, and the output of citric acid ranks first in the world. At present, the raw material commonly used in the industrial production of citric acid is mainly corn. The production of citric acid with corn as a raw material has the advantages of low energy consumption, low pollution, and high benefit. More and more The more it is favored by citric acid production enterprises, and the output of corn fermentation waste residue is also rising sharply. The main by-products produced by corn fermentation to prepare citric acid are corn starch residue and mycelium residue. Corn starch residue is the residue obtained by filtering corn after liquefaction, with a moisture ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/10
Inventor 寇光智李昌涛马菲菲孔玉魏彩霞蒋水星
Owner RIZHAO JINHE BOYUAN BIOCHEM
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