Preparation method and application of ginseng stem and leaf total saponin contrast extractive
A technology of ginseng stems and leaves and ginsenosides, which is applied in the field of preparation of reference extracts of total saponins from ginseng stems and leaves, can solve the problems of high price and shortage of component chemical reference substances, reduce pesticide residues, promote the intensification of the traditional Chinese medicine industry, and improve the content high effect
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[0027] A method for preparing a control extract of total saponins from ginseng stems and leaves, comprising the following steps:
[0028] 1) Extraction: Cut the ginseng stems and leaves into small pieces of about 2cm, soak in water for 0.5h, add water to decoct 3 times, add 16 times the amount of water each time, decoct for 1 hour each time, combine three decoction liquids, and let stand. Filter to obtain filtrate I;
[0029] 2) Refining and purification: Pass the filtrate through a styrene skeleton D101 macroporous adsorption resin column, wash with water until it is colorless, and then elute with 60% ethanol. The ethanol elution flow rate is 1.5-2.0 column volume multiples / h. Start to collect when the color appears or when the ethanol flows out. Add 20% magnesium oxide by weight of the raw material of ginseng stems and leaves, stir, heat to boiling, cool, let stand for 12-18h, centrifuge and filter, and then filter through 0.2μm membrane frame. Filtrate II;
[0030] 4) Concentrat...
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