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Bacillus subtilis fermentation medium

A Bacillus subtilis and fermentation medium technology, applied in the field of bioengineering, can solve the problems of reducing the activity and quality stability of the inoculum, low spore formation rate, and low spore rate of the inoculum, so as to promote good growth and improve production. The spore rate and the effect of promoting feed intake

Inactive Publication Date: 2016-11-23
LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the current industrial production process, the Bacillus subtilis inoculum has the problems of low spore formation rate and high production cost; the spore rate in the inoculum is low, and bacteria without spores will die in a short time, greatly reducing The activity and quality stability of the bacterial agent

Method used

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  • Bacillus subtilis fermentation medium

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Experimental program
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Effect test

Embodiment 1

[0017] A Bacillus subtilis fermentation medium, which is composed of the following components in mass percentage: 0.5%-2% corn flour, 1%-3% peptone, 1%-5% biological nitrogen source, 0.5%-2% sucrose , magnesium sulfate 0.01%-0.1%, manganese sulfate 0.1%-1%, dipotassium hydrogen phosphate 0.1%-1%, potassium dihydrogen phosphate 0.01%-0.1%, and the balance is water.

[0018] Bacillus subtilis is cultivated by a conventional fermentation process, and the fermentation process conditions are as follows: temperature: 35-37°C, ventilation: 1:1-1.2, rotational speed: 120-150 rpm, pH value: 6.5-7.8.

Embodiment 2

[0020] A Bacillus subtilis fermentation medium, which is composed of the following components in mass percentage: 1% corn flour, 2% peptone, 2.5% biological nitrogen source, 1% sucrose, 0.05% magnesium sulfate, 0.4% manganese sulfate, phosphoric acid Dipotassium hydrogen 0.5%, potassium dihydrogen phosphate 0.05%, and the balance is water.

[0021] The fermentation process condition of the present embodiment is identical with embodiment 1.

Embodiment 3

[0023] A Bacillus subtilis fermentation medium, which is composed of the following components in mass percentage: 0.5% corn flour, 3% peptone, 1% biological nitrogen source, 2% sucrose, 0.01% magnesium sulfate, 1% manganese sulfate, phosphoric acid Dipotassium hydrogen 0.1%, potassium dihydrogen phosphate 0.1%, and the balance is water.

[0024] The fermentation process condition of the present embodiment is identical with embodiment 1.

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Abstract

The invention relates to a bacillus subtilis fermentation medium which is prepared from the following components in percentage by mass: 0.5-2% of corn flour, 1-3% of peptone, 1-5% of biological nitrogen source, 0.5-2% of sucrose, 0.01-0.1% of magnesium sulfate, 0.1-1% of manganese sulfate, 0.1-1% of dipotassium phosphate, 0.01-0.1% of monopotassium phosphate and the balance of water. The medium can promote good growth of bacillus subtilis and increase the spore production rate of bacillus subtilis while the cost is low.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Bacillus subtilis fermentation medium. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, which belongs to Gram-positive bacteria, without capsule, with perinatal flagella, and can move. As a probiotic, Bacillus subtilis has the following advantages: during the growth of the bacteria, active substances such as subtilisin, polymyxin, nystatin, and gramicidin are produced, and these active substances are effective against pathogenic bacteria or endogenous infections. The conditional pathogenic bacteria have obvious inhibitory effect; can rapidly consume free oxygen in the environment, cause intestinal hypoxia, promote the growth of beneficial anaerobic bacteria, and produce organic acids, reduce intestinal pH, and indirectly inhibit other pathogenic bacteria Growth; stimulate the growth and development of animal immune organs, activat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 刘扬科胡国春郭文江郭娟
Owner LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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