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Method for effectively inhibiting astragalus callus from browning

A technology of callus and callus induction, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems affecting the growth and differentiation of culture materials, failure of scientific research and production application, browning and death of culture materials, etc. , to achieve the effects of convenient development and utilization research, short cultivation period and high output ratio

Inactive Publication Date: 2016-11-23
甘肃省农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the use of tissue culture technology to produce secondary metabolites of Astragalus membranaceus has important research and application value in the fields of biology and medicine, but the phenomenon of browning (browning It refers to the phenomenon that during the induction and differentiation process of explants, the self-tissue releases brown substances from the surface to the medium, so that the medium gradually turns brown, and the explants also further brown and die), browning makes the explants , tissue cells, and medium browning, and inhibits many enzymes, affecting the growth and differentiation of culture materials, often causing browning and death of a large number of culture materials, leading to the failure of scientific research and production applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A method for effectively inhibiting the browning of astragalus callus, comprising the steps of:

[0061] A. Astragalus seed screening

[0062] Select Astragalus seeds with full grains, no disease, insect spots and damage, cover the mouth of the beaker with gauze, rinse with tap water for 30 minutes, and rotate the rinsed seeds up and down in the beaker.

[0063] B. Astragalus seeds soaked

[0064] Add distilled water to the beaker and soak the seeds in a water bath at 60°C for 1 hour, pour out the water, and add NaHCO with a mass concentration of 2.5%. 3 solution, soaked at 35°C for 2 hours;

[0065] C. Disinfection of Astragalus seeds

[0066] First add 75% ethanol solution for disinfection for 30 seconds, then pour out the ethanol solution, then add 2% NaClO solution for disinfection for 10 minutes, then pour out the NaClO solution, and finally add 0.1% mercury chloride solution for disinfection for 2 minutes, then pour out the chlorine Mercury solution, during wh...

Embodiment 2

[0072] A method for effectively inhibiting the browning of astragalus callus, comprising the steps of:

[0073] A. Astragalus seed screening

[0074] Select Astragalus seeds with full grains, no disease, insect spots and damage, cover the mouth of the beaker with gauze, rinse with tap water for 30 minutes, and rotate the rinsed seeds up and down in the beaker.

[0075] B. Astragalus seeds soaked

[0076] Add distilled water to the beaker and soak the seeds in a water bath at 60°C for 1 hour, pour out the water, and add NaHCO with a mass concentration of 2.5%. 3 solution, soaked at 35°C for 2 hours;

[0077] C. Disinfection of Astragalus seeds

[0078] First, add an ethanol solution with a volume concentration of 75% for disinfection for 50 seconds, then pour out the ethanol solution, then add a 2% NaClO solution for disinfection for 30 minutes, then pour out the NaClO solution, and finally add a 0.1% mercury chloride solution for disinfection for 5 minutes, then pour out the ...

Embodiment 3

[0085] A method for effectively inhibiting the browning of astragalus callus, comprising the steps of:

[0086] A. Astragalus seed screening

[0087] Select Astragalus seeds with full grains, no pest spots and damage, cover the mouth of the beaker with gauze, rinse with tap water for 30 minutes, and rotate the rinsed seeds up and down in the beaker;

[0088] B. Astragalus seeds soaked

[0089] Add distilled water to the beaker and soak the seeds in a water bath at 60°C for 1 hour, pour out the water, and add NaHCO with a mass concentration of 2.5%. 3 solution, soaked at 35°C for 2 hours;

[0090] C. Disinfection of Astragalus seeds

[0091] First add 75% ethanol solution for disinfection for 40 seconds, then pour out the ethanol solution, then add 2% NaClO solution for disinfection for 20 minutes, then pour out the NaClO solution, and finally add 0.1% mercury chloride solution for disinfection for 3 minutes, then pour out the chlorine Mercury solution, during which the con...

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Abstract

The invention relates to the technical field of Chinese medicinal material processing, in particular to a method for effectively inhibiting astragalus callus from browning. The method includes the following steps of astragalus seed selection, astragalus seed soaking, astragalus seed disinfection, cleaning and inoculated culture, astragalus callus induced culture and multiplication culture. Achievements of the method are obtained on the basis of a great deal of experimental studies and continuous exploration and optimization, astragalus callus induction rate reaches 100% in the ideal induction and multiplication medium, callus grows vigorously, multiplication rate is high, and fresh weight of callus is increased to 3.27 times of that before culture via 15d culture. The method is fast in cell multiplication, short in culture cycle, capable of culturing 2 generations easily within one month, high in output ratio and good in repeatability, has the advantages of industrialized production, automatic control and the like, and is convenient for development and utilization research on secondary metabolites of astragalus in the fields of biology and medicine.

Description

technical field [0001] The invention relates to the technical field of processing traditional Chinese medicinal materials, in particular to a method for effectively inhibiting the browning of astragalus callus. Background technique [0002] Astragalus membranaceus, a traditional Chinese medicine, is a commonly used traditional medicinal plant in my country. It has the effects of invigorating qi and solidifying the surface, diuresis, draining pus, astringing sores and promoting granulation. Mongolian Astragalus membranaceus is the best of Astragalus membranaceus. In recent years, with the increasing sales of health care medicines, the dosage of Astragalus membranaceus is increasing. However, due to long-term large-scale and unrestrained excavation, the protection of natural resources has not been paid attention to. The resources of wild Astragalus membranaceus are almost exhausted. Astragalus resources are very rare. The active ingredients in Astragalus mongolica can be obta...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001
Inventor 王红梅石有太陈玉梁刘新星李淑洁王立光李静雯
Owner 甘肃省农业科学院生物技术研究所
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