A high-efficiency Burkholderia strain for degrading picolinic acid and its application

A technology of Burkholderia and picolinic acid, applied in the field of bioengineering, can solve the problems of strain activity inhibition, inhibition, inability to effectively remove picolinic acid, etc., to achieve the effect of enhancing processing capacity and broad application potential

Active Publication Date: 2019-07-23
XI AN JIAOTONG UNIV
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  • Abstract
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Problems solved by technology

Under strong acidic conditions, the growth of picolinic acid-degrading bacteria reported in the literature is significantly inhibited, so picolinic acid cannot be effectively removed
In addition, the picolinic acid-degrading bacteria reported in the literature usually show high activity between 25 and 30°C, while the activity of the strains is significantly inhibited under low temperature conditions, that is, when the temperature is ≤20°C
Therefore, the picolinic acid degrading bacteria reported in the literature are difficult to be used in low temperature environment

Method used

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  • A high-efficiency Burkholderia strain for degrading picolinic acid and its application
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  • A high-efficiency Burkholderia strain for degrading picolinic acid and its application

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the technical scheme and the accompanying drawings.

[0027] 1, the Burkholderia provided by the present invention, its screening steps are as follows:

[0028] Aerobic activated sludge samples were collected from the aeration tank of a pharmaceutical factory in Xi'an, and used as a source of bacteria for domestication and cultivation. Add 0.2% sodium pyrophosphate to 50mL of activated sludge as a deflocculant, shake on the shaker for 5-10min to break up the activated sludge flocs, take 5mL of activated sludge, add 95mL of inorganic salt medium to shake In the bottle, add picolinic acid as the only carbon, nitrogen, and energy source, wherein the concentration of picolinic acid is 100 mg / L, and culture on a shaking table at a temperature of 30° C. and a rotation speed of 180 r / min. Inorganic salt medium composition: Na 2 HPO 4 12H 2 O, 7g / L; KH 2 PO 4 , 1g / L; CaCl 2 2H 2 O, 10mg / ...

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Abstract

The invention provides a strain of Burkholderia bacterium capable of efficiently degrading picolinic acid and an application thereof, belonging to the technical field of bioengineering. The present invention isolates and screens a strain of picolinic acid degrading bacteria, Burkholderia sp., which is classified and named as Burkholderia sp. and is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC NO.10698. The advantage of the present invention is that the Burkholderia can effectively degrade picolinic acid under higher acidity or lower ambient temperature, and lower ambient temperature and strong acid conditions; the Burkholderia can be used as a bioaugmentation preparation The aerobic biological treatment of picolinic acid wastewater in high acidity and low temperature environment has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Burkholderia strain that efficiently degrades picolinic acid and its application. Background technique [0002] Picolinic acid and its derivatives are widely used in the production of medicines, pesticides and daily chemicals, and the widespread use of picolinic acid leads to environmental pollution. Picolinic acid wastewater can be treated by aerobic biological method. At present, the microbial resources for aerobic degradation of picolinic acid mainly include Arthrobacter, Mycobacterium and Streptomyces. There are few reports on strains of other genera. [0003] The most important use of picolinic acid in industry is the production of medicines and pesticides. The resulting wastewater has a high acidity and a pH value between 4 and 6. Under strong acidic conditions, the growth of picolinic acid-degrading bacteria reported in the literature is significant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 郑春莉沈振兴何炽刘萍萍刘红霞冯珊珊王巧蕊
Owner XI AN JIAOTONG UNIV
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