Tissue culture method for gesneriaceae plants

Abstract

The invention relates to the field of plant tissue culture and discloses a tissue culture method for gesneriaceae plants. The method comprises the following steps: taking an explant of a gesneriaceae plant and sterilizing; inoculating to a specific initial induction medium, performing dark culture and inducing callus, and then performing light culture and dividing into adventitious buds; inoculating the sprout tubers of the divided adventitious buds to the specific initial induction medium for performing enrichment culture, thereby obtaining available seedlings of multiple shoots; transplanting the available seedlings to a specific rooting culture medium for performing rooting culture, thereby acquiring complete aseptic seedlings. According to the invention, the specific initial induction medium, subculture medium and rooting culture medium are selected, a tissue culture technique is utilized and a new method is adopted for acquiring the aseptic seedlings of various gesneriaceae plants including primulina hance, Wuxuan primulina hance, Yongfu primulina hance, hemiboea cavaleriei var. paucinervis, officinal primulina hance, plantago asiatica, and the like, and a stable and effective tissue culture rapid propagation system is established.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for plant tissue culture of Gesneriaceae. Background technique [0002] The Gesneriaceae (Gesneriaceae) plants are mostly perennial herbaceous plants with a wide variety. There are about 140 genera and more than 2,000 species in the world. There are 58 genera and 463 species in my country. Some species are resistant to drought, shade and humidity, some have high medicinal value, some have rich plant type, leaf shape and flower shape, beautiful flowers and leaves, high ornamental value, and have a certain indoor ornamental value. Potted flower development prospects; but most species have not yet entered the commercial development stage, and due to their own genetic defects and over-exploitation of wild resources, many Gesneriaceae plants with high application value are on the verge of sterilization. The use of tissue culture rapid propagation technology is conducive t...

AI Technical Summary

Problems solved by technology

[0003] At present, there have been many reports on the tissue culture technology of Gesneria, but in many existing technologies, the differentiation rate of the first-generation cultured adventitious buds and the proliferation coefficient of the subculture culture are relatively low, and the rooting rate has not been able to reach 100%. %, as disclosed in the patent CN102144556A, in the method for the tissue culture and rapid propagation of the Yasuosaurus lettuce, its adventitious bud differentiation rate is only 31.7%, and the reproduction coefficient is only 4.6 / 60d, and the rooting rate is 90.7%; The propagation method of Brassica sativa, the multiplication factor of the subculture culture recorded in it is 3.6 times; CN105325301A discloses a method for the rapid propagation of the two-step seedling formation of the heart leaf floret, and the recorded adventitious bud differentiation The highest rate is 86.9%, the highest multiplication coefficient is 6.1 / 50d, and the highest rooting rate is 89.5% / 30d

In the tissue culture and rapid propagation method disclosed in CN104823855A, the proliferation coefficient is 15, although it has been improved, it is still not high

In addition, there are many tissue rapid propagation techniques for Gesneriaceae plants, but due to the unsatisfactory selection and matching of the medium, during the culture process, the callus induction rate, adventitious bud differentiation rate, and proliferation of the culture increased. The effect of each stage such as coefficient and rooting rate is poor

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