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Microencapsulation compound micro-ecological preparation for aquatic feed and preparing method

A composite micro-ecology, micro-encapsulation technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that need to be developed, improve the tolerance and the concentration of live bacteria, and strengthen the probiotic effect. , the effect of enhancing the probiotic effect

Pending Publication Date: 2016-11-09
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application research on the microencapsulation technology of microecological preparations for aquatic feeding, especially the research on microencapsulation of compound microecological preparations, needs to be carried out urgently.

Method used

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  • Microencapsulation compound micro-ecological preparation for aquatic feed and preparing method
  • Microencapsulation compound micro-ecological preparation for aquatic feed and preparing method
  • Microencapsulation compound micro-ecological preparation for aquatic feed and preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Preparation of microencapsulated compound probiotics

[0058] 1. Culture the bacteria in liquid and prepare the activation solution.

[0059] Rhodopseudomonas palustris: Take glycerol tubes of Rhodopseudomonas paluscans stored at -80°C. After thawing, add 2% by volume to the test tube containing Van Niel's liquid medium. Illuminate with a 60W incandescent lamp, perform anaerobic cultivation at a temperature of 28° C., and after static cultivation for 30 hours, obtain an activated solution of Rhodopseudomonas palustris.

[0060] Bacillus subtilis and Bacillus licheniformis: Glycerol tubes of Bacillus subtilis and Bacillus licheniformis stored at -80°C were taken respectively. After thawing, add 2% by volume to test tubes containing BPY liquid medium. The test tube was placed on a shaker with a rotation speed of 180 rpm and a temperature of 28° C., and cultured for 18 hours to obtain Bacillus subtilis activation solution and Bacillus licheniformis activation soluti...

Embodiment 2

[0081] (1) Preparation of microencapsulated compound probiotics for aquatic feeding

[0082] 1. Culture the bacteria in liquid and prepare the activation solution.

[0083] Rhodopseudomonas palustris: Take glycerol tubes of Rhodopseudomonas paluscans stored at -80°C. After thawing, add 1% volume to the test tube containing Van Niel's liquid medium. Illuminated with a 40W incandescent lamp, anaerobically cultured at a temperature of 25° C., and left to culture for 36 hours to obtain an activated solution of Rhodopseudomonas palustris.

[0084] Bacillus subtilis and Bacillus licheniformis: Glycerol tubes of Bacillus subtilis and Bacillus licheniformis stored at -80°C were taken respectively. After thawing, add 1% by volume to test tubes containing BPY liquid medium. The test tube was placed on a shaker with a rotation speed of 160 rpm and a temperature of 25° C., and cultured for 20 hours to obtain Bacillus subtilis activation solution and Bacillus licheniformis activation so...

Embodiment 3

[0108] (1) Preparation of microencapsulated compound probiotics for aquatic feeding

[0109] 1. Culture the bacteria in liquid and prepare the activation solution.

[0110] Rhodopseudomonas palustris: Take glycerol tubes of Rhodopseudomonas paluscans stored at -80°C. After thawing, add 3% volume to the test tube containing Van Niel liquid medium. Illuminate with a 100W incandescent lamp, perform anaerobic cultivation at a temperature of 30° C., and after static cultivation for 24 hours, obtain an activated solution of Rhodopseudomonas palustris.

[0111]Bacillus subtilis and Bacillus licheniformis: Glycerol tubes of Bacillus subtilis and Bacillus licheniformis stored at -80°C were taken respectively. After thawing, add 3% by volume to test tubes containing BPY liquid medium. The test tube was placed on a shaker with a rotation speed of 200 rpm and a temperature of 30° C., and cultured for 12 hours to obtain Bacillus subtilis activation solution and Bacillus licheniformis ac...

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Abstract

The invention discloses a microencapsulation compound micro-ecological preparation for aquatic feed and a preparing method. The microencapsulation compound micro-ecological preparation is mixed, fermented and cultivated through bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris and lactobacillus bulgaricus seed liquid, then mixed bacterium liquid serves as a core material, sodium alginate is selected as a wall material, vegetable oil with dispersed tween-80 serves as an oil phase, vegetable oil with dispersed calcium chloride and dispersed tween-80 serves as a cross-linking agent, a calcium chloride solution serves as a curing agent, and the microencapsulation compound micro-ecological preparation is prepared with the emulsifying method. The microencapsulation compound micro-ecological preparation prepared with the method is suitable for being added into the aquatic feed and stirred to be even to be fed, the tolerance of probiotics for the poor environment and the concentration of living bacteria are improved, the shelf life of a product is prolonged, and the prebiotics effect can be reinforced accordingly.

Description

technical field [0001] The invention relates to a microecological preparation for aquatic feeding, in particular to a microencapsulated composite microecological preparation for aquatic feeding and a preparation method thereof. Background technique [0002] As a kind of green feed additive, the application of microecological preparations for aquaculture in aquaculture has been paid more and more attention. Its product type has gradually developed from a single strain to a composite strain composed of multiple strains. However, there has been no major breakthrough in the product formulation and production process of aquatic compound probiotics in recent years. Most complex bacteria products are just a random mixture of several bacteria, resulting in products that do not conform to the use environment, and the growth and reproduction conditions of the strains are affected. [0003] In addition, different strains will be affected by various environmental factors inside and ou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N11/10C12N11/04A23K50/80A23K40/00A23K10/18C12R1/125C12R1/10C12R1/01C12R1/225
CPCY02A40/818C12N1/04C12N11/04C12N11/10
Inventor 孙敬锋孙金辉陈丰吕爱军
Owner TIANJIN AGRICULTURE COLLEGE
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