Liquid culture device for hairy roots

Abstract

A liquid culture device for hairy roots comprises a pot body. The upper portion of the pot body is provided with a shrunk pot opening, an opening sealing cover is arranged at the pot opening, and an opening sealer is arranged at the center of the opening sealing cover; the two shoulder walls of the pot body are provided with a gas inlet and a liquid supplementing port respectively, and the liquid supplementing port is communicated with the interior of the pot body through a feeding pipe; a groove is formed in the center of the bottom of the pot body, and a gas generation device is fixed in the groove and connected with the gas inlet through a ventilation pipe. The culture device is made of a transparent high-temperature-resistant material, and the growth state of the hairy roots in the pot body can be observed in real time; the gas generation device is arranged at the bottom of the pot body, the fixing groove is formed, and therefore top-blowing oxygen supply is achieved; gas is exhausted through a filter membrane arranged on the opening sealing cover, and compared with the traditional design that a vent hole is formed in the bottom of a culture device, liquid leakage caused by the fact that an opening is formed in the bottom can be prevented, and extraneous contamination can be prevented; all joints in the pot body are in an arc shape, no dead corner exists in the pot body, therefore, impurity residues in the culture device are prevented, and liquid eddy can be reduced, so that growth of the hairy roots is stabilized.

Description

technical field [0001] The invention relates to a plant tissue culture device, in particular to a culture device for cultivating hairy root growth, which belongs to the field of reactor engineering of plant culture technology. Background technique [0002] The use of hairy root culture to produce medicinal plant secondary metabolites has great production potential, and the development of a bioreactor suitable for hairy root culture is the basis and key to its industrialization. At the level of shake flask culture, domestic and foreign scholars have established more than 100 hairy root culture systems and obtained different active compounds, but these hairy roots are finally used for expanded cultivation or industrial production. This is mainly due to: (1) Hairy roots are very easy to coalesce into tight balls or clusters, resulting in a decrease in dissolved oxygen inside the culture, leading to accelerated aging or even death of internal hairy roots, which ultimately affect...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the oxygen supply method of the hairy root culture device in the prior art can easily cause the hairy root to be mechanically damaged, which is not conducive to the stretching and growth of the hairy root, the diffusion of dissolved oxygen is uneven, and it is easy to cause the culture solution to leak. The problem of exogenous pollution caused by adding nutrient solution, the present invention intends to provide an incubator with uniform diffusion of dissolved oxygen, which is more conducive to the growth of hairy roots

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