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New histone methyltransferase 1 expression level reducing function of karounitriol

A new functional technology of Trichosanthin diol, which is applied in the direction of drug combination, medical preparations containing active ingredients, steroids, etc.

Active Publication Date: 2016-11-09
南京思恩倍医药科技有限公司
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Problems solved by technology

However, the functional effects of the active ingredients of Trichosanthes chinensis, especially Trichosanthene diol, on the central nervous system have not been reported yet.

Method used

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  • New histone methyltransferase 1 expression level reducing function of karounitriol

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Experimental program
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Effect test

Embodiment 1

[0015] The detection process of the physiological activity of trichosanthene diol is:

[0016] The SD rats born within 24 hours were sacrificed and the hippocampus tissue was taken for primary culture. Put the hippocampus tissue on a 200-mesh sieve, use ophthalmic scissors to cut small pieces and sieve, pour off the supernatant after centrifuging at 1000rpm for 8 minutes, add high-sugar DMEM medium and 10% fetal bovine serum, and plant after pipetting 6-well plate. Culture condition is 37 o C 5% level of carbon dioxide. The medium was changed every three days. When cultured to the 7th day, the primary cultured cells were added to three wells of primary cultured cells with a final concentration of 50 μM Trigalendiol, and the other three wells were added with the same volume of sterile water as a control group. When the culture was continued for 14 days, the cells were recovered. The total mRNA in the cells was extracted by conventional technical means in the field, and rev...

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Abstract

Karounitriol is an important active ingredient from Semen Trichosanthis which is a traditional Chinese medicinal material. The invention introduces a new function of the karounitriol. Through action experiment on primary cultured neuron of a rat, the expression level of an EZH1 gene can be reduced by adding the karounitriol. The EZH 1 is a key enzyme causing methylation of histone H3, methylation of histone is an important constituent part of epigenetics, and change of the histone shows that a nervous system has global stress reaction on the karounitriol, and the karounitriol has remarkable regulating effect on activity of the nervous system.

Description

technical field [0001] This application belongs to the technical field of new functions of active ingredients of medicinal materials, and in particular relates to an active ingredient isolated from the Chinese herbal medicine Trichosanthes ziponaris, which can reduce hippocampal neuron EZH1 New features of gene expression levels. Background technique [0002] Histone methyltransferase 1, EZH1, is a key enzyme that catalyzes the methylation of histone H3. Histone methylation is an important level of epigenetic regulation. The methylation of lysine 27 of histone H3 catalyzed by EZH1 is an important means for the body to regulate gene expression, and it is also an important part of the histone code. component. Under different physiological and pathological backgrounds, the change of EZH1 expression indicates the overall change of the target gene, reflecting the overall adaptive or damaging changes of the body. [0003] Melon wilt is the dry mature seed of Trichosanrhes kiril...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P25/00C07J63/00A61K36/428
CPCA61K31/56C07J63/008A61K36/428
Inventor 不公告发明人
Owner 南京思恩倍医药科技有限公司
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