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Histone methylated transferase and its encoding gene and application

A technology of histone methylation and transferase, applied in the field of histone methyltransferase and its encoding gene and application, can solve the problem of Arabidopsis dwarf plant, Arabidopsis meristem development retardation, leaves and roots growth retardation

Inactive Publication Date: 2010-01-06
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overexpression of SUVH2 can lead to dwarf Arabidopsis plants; in tobacco, overexpression of NtSET1 can also cause leaf and root growth retardation; OsSET1 in rice is another SET domain protein, and overexpression of OsSET1 gene can cause Arabidopsis delayed apical meristem development

Method used

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  • Histone methylated transferase and its encoding gene and application
  • Histone methylated transferase and its encoding gene and application
  • Histone methylated transferase and its encoding gene and application

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1, the acquisition of histone methyltransferase SDG714

[0037] 1. Discovery of histone methyltransferase SDG714

[0038]Data mined through the http: / / www.chrombd.org database showed that there are 38 SET domain proteins in rice, named SDG701 to SDG741. Phylogenetic analysis showed that SDG714 was most similar to KYP / SUVH4 in Arabidopsis, and KYP / SUVH4 belonged to Su(var)3-9 family. SDG714 encodes 663 amino acids (sequence 1 in the sequence listing), contains a conserved SET domain of about 130 amino acids (from the 480th-640th amino acid residue at the amino terminal of sequence 1) and two conserved cysteine ​​moieties body, namely pre-SET (from the 375th to 478th amino acid residue in SEQ ID NO: 1) and post_SET (from the 641st to 663rd amino acid residue in SEQ ID NO: 1). The YDG domain (from the 182nd to the 346th amino acid residue at the amino terminal of SEQ ID NO: 1) is very conserved in the SUVH family of higher plants, but it does not appear in animal...

Embodiment 2

[0050] Example 2, activation of transposition of Tos17 in rice

[0051] 1. Construction of SDG714 RNAi expression vector

[0052] In order to understand the function of SDG714 in vivo, SDG714 was knocked out by RNAi technology, and the N-terminal of SDG714 and a part of YDG non-conserved part were used as RNAi regions.

[0053] The method of constructing the RNAi expression vector of SDG714 is as follows: extract rice total RNA, use oligo d(T) as a primer, reverse transcribe into cDNA, use primers CX52 (5'-ATTctcgagGCAACTTGTATTGTCATGTCGGG-3') and CX53 (5'-AGCagatctGGGAGCTTCAGCACGAGTAA- 3') PCR amplified deoxyribonucleotides from the 712th to the 1085th position of the 5' end of the sequence 2. After the PCR product was digested with BglII and XbalI, it was inserted into the pUCRNAi vector digested by XhoI / BglII and BamHI / SalI in both forward and reverse directions to obtain the recombinant vector pUC-SDG714RNAi, and then pUC-SDG714RNAi was digested with PstI. The forward and...

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Abstract

The invention discloses a histone methylating transferase and coded gene and application, which contains protein (a) or protein (b), wherein the (a) is composed of residue sequence of amino acid in the sequence list 1; (b) protein is composed of (a) derivatized plant in the sequence list 1; the transferase controls the swivelling activity of Tos17, which makes Tos17 insert new location to produce mutant.

Description

technical field [0001] The invention relates to a histone methyltransferase, its coding gene and application. Background technique [0002] In 1951, Barbara Mclintock first discovered the control element in maize, which was later named transposable element or transposon (transposon). A transposon is a mobile DNA sequence in the genome that can "jump" from one location of the genome to another through a series of processes such as cutting and reintegration. This element can not only be used to analyze some phenomena caused by molecular effects in biological genetic evolution, but also provides a powerful tool for genetic engineering and molecular biology research. Isolation and cloning of plant genes is called transposon tagging, also known as transposon tagging. The principle is to use the insertion of a transposon to cause gene mutation. Based on the transposon sequence, a clone with this transposon is screened from the gene library of the mutant strain. It must contain a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/82
Inventor 曹晓风丁勇刘春艳储成才
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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