Subculture medium for blueberries and preparation method of medium

Abstract

The invention discloses a subculture medium for blueberries. The medium contains macroelement mother liquor, microelement mother liquor, iron salt mother liquor, calcium salt mother liquor, organic matter mother liquor and hormone mother liquor, wherein the organic matter mother liquor in every one liter of the subculture medium for the blueberries is prepared from 100 mg of inositol, 1 mg of niacin, 1 mg of vitamin B1, 2mg of vitamin B6 and 2 mg of vitamin C, and the hormone mother liquor in every one liter of the subculture medium for the blueberries is prepared from 4 mg of zeatin. A plant growth hormone, namely, the zeatin, is added to the subculture medium, so that blueberry tissue cultured plants can grow well, have many ramets and root easily, transplantation of the blueberries into fields after culture is facilitated, and the survival rate of the blueberries in the fields is increased; meanwhile, under the assistance of a matched amount of the vitamin C, the vitrification effect of the zeatin on the blueberries can be eliminated, better growth of the blueberry plants can be promoted, and cultured seedlings grow stronger, are not browned to death easily and have greener and flat leaves.

Description

technical field [0001] The invention relates to a culture medium, in particular to a subculture medium. Background technique [0002] Blueberry is a fruit with very high nutritional value. The pulp is rich in nutrients such as vitamins, protein, dietary fiber, fat and minerals, which is much higher than other fruits. Among them, the unique anthocyanin in blueberry The proportion of pigment in all fruits is very high. In the cultivation of blueberries, the requirements for soil are relatively high. Soil organic matter content and pH value are important factors for the survival of blueberry cultivation. Generally, soil with an organic matter content of 5% and a pH value of 4.0-5.5 is required. At present, a large number of blueberry seedlings are propagated using tissue culture methods. Similarly, in tissue culture, blueberries also have relatively high requirements for the medium, requiring high organic matter content and weakly acidic pH. [0003] The medium commonly used ...

AI Technical Summary

Problems solved by technology

However, in the tissue culture of blueberries, whether using MS or WPM medium, the effect is not very good

Although many blueberry cultures currently reported use WPM medium, they are all for a certain blueberry variety. Therefore, the reported medium formulations are not universal for most blueberry varieties

[0004] Zeatin is generally used in the tissue culture process to promote cell division and promote plant division, but the concentration of zeatin used is too high, or the frequency of zeatin used in the subculture process is too frequent, which may easily cause vitrification of blueberry plants. Vitrified blueberry seedlings have no wax on the surface of stems and leaves, high levels of polar compounds in the body, poor cell water holding capacity, strong transpiration, and cannot be transplanted normally, resulting in a great waste of manpower, material and financial resources , is currently the biggest obstacle to the production of tissue cultured seedlings in blueberry tissue culture

However, if zeatin is not used or the concentration of zeatin is too low, blueberry plants cannot grow normally, and in severe cases, blueberry plants will die.

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